Intervención educativa para mejorar la calidad del diagnóstico coproparasitológico en la red de salud de Ciudad Habana, Cuba

Abstract

The aim of this study was to evaluate the effectiveness of an educational program carried out in 1993. The intervention took place after the first external quality assessment in coproparasitology, conducted in 77 laboratories of Havana City. Centers receiving training were compared with those that did not, and better results were obtained in the municipalities of 10 de Octubre, Plaza, and Cerro, as well as in all laboratories that sent people to training. Better diagnosis was found for the helminths Trichuris trichiura, Taenia sp., and Fasciola hepatica as well for the protozoans Blastocystis hominis and Endolimax nana in the laboratories that received training. The laboratories that received training had significantly higher scores than those which did not. The results point to the effectiveness of the educational intervention. We recommend external quality assessment in coproparasitology as well as ongoing, mandatory technical education, held at regular intervals. Such measures should assure steady improvement in diagnosis of intestinal parasites by the health services network.

Padronização do diagnóstico de Blastocystis hominis por diferentes técnicas de coloração

“…Como vantagens da utilização de qualquer uma das técnicas de coloração permanente (MGG e Gram), podemos citar a sua utilização rotineira nos laboratórios clínicos, seja no setor de Hematologia ou no setor de Bacteriologia, o que dispensa o gasto econômico do laboratório com a montagem de uma técnica de coloração específica 25 . Observamos também que utilizando o método de coloração de MGG há uma maior facilidade, já que, durante a coloração de Gram, temos a etapa da descoloração que é crítica, pois a exposição prolongada ao solvente pode provocar a remoção do cristal violeta do interior dos núcleos periféricos do Blastocystis hominis, ou ainda, se não houver a descoloração necessária não iremos observar o vacúolo central, prejudicando assim a visualização do parasita em questão 22,26 .…”

Evaluation of Clinical Laboratories in Diagnosing Intestinal Parasitic Infections

“…Most of the studies are performed in hematology, biochemistry and microbiology laboratories (Sardiñas et al, 2016;Mao et al, 2018;Souza et al, 2018). The few studies published on parasitology laboratories are mainly from Latin America (Ayala & Sánchez, 1974;Castro et al, 1995, Laird et al, 1997, Núñez et al, 1998Núñez & Finlay, 2001;Blanco et al, 2013;Jemere et al, 2018).…”