Interstitial Hyperthermia Using Magnetite Cationic Liposomes Inhibit to Tumor Growth of VX-7 Transplanted Tumor in Rabbit Tongue.

Abstract

We previously developed a new system of magnetic induction interstitial hyperthermia, implant heating system (IHS), and applied to oral cancer control. To overcome the limitations of this system, the present study dealt with new magnetic induction hyperthermia using Magnetic Cationic Liposomes (MCLs) which are injected into a tumor. We prepared a tongue tumor model using the transplantable rabbit VX-7 cells. MCL was injected into the tumor margins to heat the tumor selectively and induce blood vessel damage by heating. Two separated MCL injection sites were made by using Micro syringe pump and sandwiched the tumor on both sides. The rabbit in group I (n = 4) were performed hyperthermia, while those in group II (n= 4) were not subjected to magnetic field irradiation as the control. In the experimental rabbits, the temperature in the tumor was as high as 43.0°C. The tumor volumes of irradiated rabbits were significantly smaller than those of the non irradiated ones. The transplanted tumor of experimental rabbits almost completely disappeared following 18 day after treatment. The tongues, removed from irradiated rabbits on 28th day after irradiation, showed no tumor cells and fibrous tissue replacement completely occurred in the implanted tumor area. Tongue muscle adjacent to the MCL injection sites did not exhibit any evidence of inflammation or necrosis. The present study confirmed the effectiveness of interstitial hyperthermia using MCLs against oral carcinoma.

Targeted hyperthermia using magnetite cationic liposomes and an alternating magnetic field in a mouse osteosarcoma model

“…Our results showed that hyperthermia using MCLs exerts a suppressive effect in an osteosarcoma model, and has also been shown to be effective with B16 mouse melanoma, 21 T-9 rat glioma 22 and VX-7 squamous cell carcinoma in rabbit tongue. 23 The prognosis of patients with osteosarcoma has improved markedly following the introduction of effective chemotherapy. However, the prognosis remains poor in the presence 24,25 of an unresectable primary tumour arising in the axial skeleton or presenting with distant metastases.…”

Magnetic nanomaterials for hyperthermia-based therapy and controlled drug delivery

“…Recently, a number of in vivo studies using animal models were undertaken where MNPs were injected into a tumor followed by AMF exposure and resulting changes in the tissue and organs were investigated. These include C3H mouse mammary carcinoma [63] , mouse EL4 T-lymphoma [11] , MX11 mouse sarcoma [64] , human prostate cancerous and bone marrow cells in transgenic mice [46] , EL4 mouse T-lymphoma [65] , T-9 rat glioma cells [66] , B16 mouse melanoma [67] , MM46 mammary and skin carcinoma [68] , murine B16-F10 mouse melanoma [69] , cat mammary tumor gland [70] , Os515 hamster osteosarcoma [71] , VX-7 squamous cell carcinoma in rabbit tongue [72] , DMBA induced rat mammary carcinoma [73] , and human glioma cells. [74] Most of the studies though provided positive evidence for decrease in tumor size due to hyperthermia, there is a clear lack of complete information on host and material response.…”

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“…Based on this feature, MCLs can be highly superior heating mediators. We previously demonstrated the efficacy of MCL-mediated hyperthermia in animals with several types of tumors, including B16 mouse melanoma [19,20], T-9 rat glioma [11,21], renal cell carcinoma [22], and VX-7 squamous cell carcinoma in rabbit tongue [23]. We also reported complete regression of mouse mammary carcinoma, larger than 15 mm in size, by frequent repeated hyperthermia [24].…”