Interstitial diffusion of macromolecules in the rat mesentery

Fox, James R.; Wayland, Harold

doi:10.1016/0026-2862(79)90033-5

Cited by 138 publications

(55 citation statements)

References 33 publications

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“…Another possible source for protein gradients would be sieving through the restrictive gel matrix . This would occur if the size of the intersticies between the matrix fibers were of the order of the pore sizes in the capillary, as has been suggested for the rat mesentery (Fox and Wayland, 1979). Since albumin is smaller in size than IgG, the interstitial sieving of albumin would be less than for IgG.…”

mentioning

confidence: 93%

“…However, these techniques may be too coarse to detect small regional protein concentration gradients within the matrix. Fox and Wayland (1979) studied the diffusion rates of fluorescein-labeled albumin and large size dextrans in the rat mesentery. They found that the restricted diffusion coefficient for albumin was about 7% of the free diffusion coefficient and that the interstitial matrix behaved as if it had between 50 and 70 A pores.…”

mentioning

confidence: 99%

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Changes in interstitial volume and masses of albumin and IgG in rabbit skin and skeletal muscle after saline volume loading.

Mullins¹,

Bell²

1982

Circulation Research

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SUMMARY. This study was performed on the hindpaws of anesthetized rabbits to determine the effects of acute saline expansion (10% body weight) on the extravascular distribution of water and plasma proteins. Prenodal lymph was collected separately from skin and muscle of the hindlegs. In samples of excised heel skin and gastrocnemius muscle, the extracellular and plasma spaces were measured with 51 Cr EDTA and 125 I albumin, respectively. The albumin and IgG spaces were calculated from measurements of the endogenous albumin and IgG concentrations in samples of plasma, lymph, and tissue extracts, by immunochemical techniques. Four hours after expansion, lymph flow from both tissues was more than 3 times greater than control, while the interstitial volume was increased by 20% in skin and 2.3 times for muscle. The elevated lymph flow was accompanied by a decrease in the lymph:plasma concentration ratio. The extravascular mass of albumin was 9.36 ± 0.61 and 3.93 ± 0.31 mg/g dry weight for control skin and skeletal muscle, respectively. The IgG mass was 2.62 ± 0.47 and 0.717 ± 0.084 mg/g dry weight for skin and muscle. Saline expansion resulted in a 41% increase in the extravascular albumin mass in muscle and no change in albumin mass in skin. The extravascular IgG mass increased by 58% in skin and 49% in muscle. The calculated excluded volume fraction for albumin decreased in both tissues and for IgG decreased in muscle. In skin, the IgG-excluded volume fraction could not be calculated after expansion since the apparent tissue concentration was greater than lymph, indicating interstitial concentration gradients for this molecule. Acute saline expansion resulted in a shift of plasma proteins from plasma to the extravascular space of skin and skeletal muscle. (Circ Res 51: [305][306][307][308][309][310][311][312][313] 1982)

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mentioning

confidence: 93%

mentioning

confidence: 99%

Changes in interstitial volume and masses of albumin and IgG in rabbit skin and skeletal muscle after saline volume loading.

Mullins¹,

Bell²

1982

Circulation Research

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“…The schematic drawing of the model geometry, the same as in Fox and Wayland's experiment, 14 is shown in Fig. 1.…”

Section: Model Geometrymentioning

confidence: 99%

“…Transport in Region C can be approximated by a 1-D transport model averaged across the height (thickness) of the tissue layer. 14,17,18,23 Interstitium carries negative charge with charge density, C m,t, due to the existence of glycosaminoglycans. For simplicity, we neglect the charge in near tissue region, Region B.…”

Section: Model Geometrymentioning

confidence: 99%

“…7,31 The aim of this study is to develop a mathematical model to quantitatively depict how interstitial charge, in the forms of electrical partition and electrical interaction, affects the transport of charged macromolecules. By applying our model predictions to an in vivo experiment performed by Fox and Wayland,14 another aim is to test the hypothesis that for connective tissue like mesentery, the tissue charge effect due to the electrical partition is dominant over that due to the electrical interaction for the transport of a charged macromolecule like albumin.…”

Section: Introductionmentioning

confidence: 99%

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A Time-Dependent Electrodiffusion-Convection Model for Charged Macromolecule Transport Across the Microvessel Wall and in the Interstitial Space

Chen

2009

Cel. Mol. Bioeng.

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Abstract-The extravascular matrix carries negative charge due to its glycosaminoglycans (GAGs) composition. Previous experiments have demonstrated that the negative charge affects transvascular passage and interstitial accumulation of charged molecules in both normal and pathological tissues. In the present study, we thereby developed an electrodiffusionconvection model to investigate the mechanisms by which the negatively charged tissue matrix regulates the interstitial transport of charged macromolecules. Our model predictions demonstrated that the tissue diffusion coefficient of negatively charged albumin (net charge = À19) in rat mesentery is comparable to that of neutral dextran with equivalent hydrodynamic radius. The discrepancy in their interstitial concentration profiles observed by Fox and Wayland (Microvasc. Res. 18:255-276, 1979) can be explained by the charge effect, specifically, by the electrical partition at the interface between the non-charged space at the vessel wall exit and the charged tissue space, instead of by different tissue diffusion coefficients that Fox and Wayland postulated. This charge effect induces equivalent to approximately two-fold difference in tissue diffusion coefficients of charged albumin and neutral dextran with the same free diffusion coefficients. Furthermore, our results indicate that increased filtration by increased microvessel permeability greatly enhances the accumulation of positively charged macromolecules in the interstitial space but not that of negatively charged ones.

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Permeability of Rheumatoid and Normal Human Synovium to Specific Plasma Proteins

Levick

1981

Arthritis & Rheumatism

151

116

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A method is described for the determination of the permeability of the blood-joint barrier to specific plasma proteins, using the ratio of protein concentration in synovial fluid to that in plasma. The inadequacy of the ratio per se as a direct index of permeability is discussed. Permeabilities are evaluated for the normal and rheumatoid human knee. Permeability increases in the rheumatoid knee by approximately 6 times for albumin and over 40 times for macroglobulins. The effect of protein molecular dimensions upon permeability is analyzed. Permeability shows less dependence upon solute dimensions in the rheumatoid knee than in the normal knee, i.e., molecular selectivity is reduced. From these data and synovial morphology, a twomembrane model of the blood-joint barrier is developed. The relative contribution of the component intima1 and endothelial layers to the total barrier is found to depend upon solute dimensions.

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Interstitial diffusion of macromolecules in the rat mesentery

Cited by 138 publications

References 33 publications

Changes in interstitial volume and masses of albumin and IgG in rabbit skin and skeletal muscle after saline volume loading.

Changes in interstitial volume and masses of albumin and IgG in rabbit skin and skeletal muscle after saline volume loading.

A Time-Dependent Electrodiffusion-Convection Model for Charged Macromolecule Transport Across the Microvessel Wall and in the Interstitial Space

Permeability of Rheumatoid and Normal Human Synovium to Specific Plasma Proteins

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