Interstitial cells in the vasculature

Harhun, Maksym I.; Pucovský, Vladimír; Povstyan, Oleksandr V.; Gordienko, Dmitri; Bolton, T. B.

doi:10.1111/j.1582-4934.2005.tb00352.x

Cited by 71 publications

(65 citation statements)

References 72 publications

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“…However, after the year 2000, ICC-like cells (ICC-LC) have been found outside the gastrointestinal tract (reviews in 2005: [3,4]). Our laboratory reported the identification (at actual and quasi-complete standards) of ICC-LC in human pancreas [5,6], uterus [7] and fallopian tube [8].…”

Section: Giemsa Staining 'Octopus-like'mentioning

confidence: 99%

Cajal-type cells from human mammary gland stroma: phenotype characteristics in cell culture

et al. 2005

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We report here the in vitro isolation of Cajal-like interstitial cells from human inactive mammary-gland stroma. Primary cell cultures examined in phase-contrast microscopy or after vital methylene-blue staining revealed a cell population with characteristic morphological phenotype: fusiform, triangular or polygonal cell body and the corresponding (very) long, slender, moniliform cytoplasmic processes. Giemsa staining pointed out the typical knobbed aspect of cell prolongations. Immunofluorescence (IF) showed, like in situ immunohistochemistry, that Cajal-type cells in vitro (primary cultures), expressed c-kit/CD117 and vimentin. In conclusion, the images presented here reinforce our previous hypothesis that human mammary glands have a distinct population of Cajal-like cells in non-epithelial tissue compartments. J. Cell. Mol. Med. Vol 9, No 3, 2005 pp. 748-752 Keywords: interstitial cells of Cajal Images in

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Section: Giemsa Staining 'Octopus-like'mentioning

confidence: 99%

Cajal-type cells from human mammary gland stroma: phenotype characteristics in cell culture

et al. 2005

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“…In a series of recent papers, ICC-like cells have been claimed to occur in other organs: vas deferens [25], urethra [26][27][28], bladder [29][30][31][32][33], ureter [34,35], uterus [36,37], blood vessels [38][39][40], lymphatics [41], prostate [42,43], penis [44], mammary gland [45] and pancreas [46].…”

Section: Introductionmentioning

confidence: 99%

Novel type of interstitial cell (Cajal-like) in human fallopian tube

et al. 2005

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We describe here ‐ presumably for the first time‐a Cajal‐like type of tubal interstitial cells (t‐ICC), resembling the archetypal enteric ICC. t‐ICC were demonstrated in situ and in vitro on fresh preparations (tissue cryosections and primary cell cultures) using methylene‐blue, crystal‐violet, Janus‐Green B or Mito Tracker‐Green FM Probe vital stainings. Also, t‐ICC were identified in fixed specimens by light microscopy (methylene‐blue, Giemsa, trichrome stainings, Gomori silver‐impregnation) or transmission electron microscopy (TEM). The positive diagnosis of t‐ICC was strengthened by immunohistochemistry (IHC; CD117/c‐kit+ and other 14 antigens) and immunofluorescence (IF; CD117/c‐kit+ and other 7 antigens). The spatial density of t‐ICC (ampullar‐segment cryosections) was 100–150 cells/mm2. Non‐conventional light microscopy (NCLM) of Epon semithin‐sections revealed a network‐like distribution of t‐ICC in lammina propria and smooth muscle meshwork. t‐ICC appeared located beneath of epithelium, in a 10–15μ thick ‘belt’, where 18±2% of cells were t‐ICC. In the whole lamina propria, t‐ICC were about 9%, and in muscularis ∼7%. In toto, t‐ICC represent ∼8% of subepithelial cells, as counted by NCLM. In vitro, t‐ICC were 9.9±0.9% of total cell population. TEM showed that the diagnostic ‘gold standard’ (Huizinga et al., 1997) is fulfilled by ‘our’ t‐ICC. However, we suggest a ‘platinum standard’, adding a new defining criterion ‐ characteristic cytoplasmic processes (number: 1–5; length: tens of μm; thickness: ±0.5μ; aspect: moniliform; braching: dichotomous; organization: network, labyrinthic‐system). Quantitatively, the ultrastructural architecture of t‐ICC is: nucleus, 23.6±3.2% of cell volume, with heterochromatin 49.1±3.8%; mitochondria, 4.8±1.7%; rough and smooth endoplasmic‐reticulum (1.1±0.6%, 1.0±0.2%, respectively); caveolae, 3.4±0.5%. We found more caveolae on the surface of cell processes versus cell body, as confirmed by IF for caveolins. Occasionally, the so‐called ‘Ca2+‐release units’ (subplasmalemmal close associations of caveolae+endoplasmic reticulum±mitochondria) were detected in the dilations of cell processes. Electrophysiological single unit recordings of t‐ICC in primary cultures indicated sustained spontaneous electrical activity (amplitude of field potentials: 57.26±6.56mV). Besides the CD117/c‐kit marker, t‐ICC expressed variously CD34, caveolins 1&2, α‐SMA, S‐100, vimentin, nestin, desmin, NK‐1. t‐ICC were negative for: CD68, CD1a, CD62P, NSE, GFAP, chromogranin‐A, PGP9.5, but IHC showed the possible existence of (neuro)endocrine cells in tubal interstitium. We call them ‘JF cells’. In conclusion, the identification of t‐ICC might open the door for understanding some tubal functions, e.g. pace‐making/peristaltism, secretion (auto‐, juxta‐ and/or paracrine), regulation of neurotransmission (nitrergic/purinergic) and intercellular signaling, via the very long processes. Furthermore, t‐ICC might even be uncommitted bipotential progenitor cells.

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“…The wall of the portal vein in rabbit (RPV) is structurally comparable to human PV (Harhun et al, 2005). The RPV is a spontaneously active blood vessel and the distribution of the PICCs in the wall of the RPV resembles the distribution of ICCs in the gastrointestinal (GI) tract (Harhun et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The RPV is a spontaneously active blood vessel and the distribution of the PICCs in the wall of the RPV resembles the distribution of ICCs in the gastrointestinal (GI) tract (Harhun et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…ICCs are known to be immunopositive for c-kit, a proto-oncogene that encodes a tyrosine kinase receptor (Mei et al, 2009), making it a useful marker for these cells (Ward and Sanders, 2001;Povstyan et al, 2003;Harhun et al, 2005;Sanders et al, 2006;Popescu and Faussone-Pellegrini, 2010). However, it is not mandatory for all ICCs to be labeled with c-kit antibodies (Huizinga and Faussone-Pellegrini, 2005).…”

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Extrahepatic and Intrahepatic Human Portal Interstitial Cajal Cells

Rusu

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et al. 2011

The Anatomical Record

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Portal interstitial cells of Cajal (PICCs), acting as vascular pacemakers, were previously only identified in nonhumans. Moreover, there is no evidence available about the presence of such cells within the liver. The objective of the study is to evaluate whether or not PICCs are identifiable in humans and, if they are, whether or not they are following the scaffold of portal vein (PV) branches within the liver. We obtained extrahepatic PVs and liver samples from six adult human cadavers, negative for liver disease, in accordance with ethical rules. They were stained with hematoxylin-eosin (HE) and Giemsa, and then we performed immunohistochemistry on formalin-fixed paraffin-embedded specimens for CD117/c-kit, a marker of the Cajal's cells. Immune labeling was also performed for S-100 protein, desmin, glial fibrillary acidic protein (GFAP), neurofilaments, a-smooth muscle actin (a-SMA), and CD34. c-kit-Positive PICCs were identified within the extrahepatic PV, in portal spaces, and septa. On adjacent sections, these PICCs were negative for all the other antibodies used. In conclusion, our study confirms the presence of extrahepatic PICCs on humans, which may act as a possible intrinsic pacemaker in the human PV. However, the intrahepatic PICCs, which were evidenced here for the first time, are in need for further experimental studies to evaluate their functional role. A promising further direction of the study is the PICCs role in the idiopathic portal hypertension. Anat Rec, 294:1382Rec, 294: -1392Rec, 294: , 2011. V V C 2011 Wiley-Liss, Inc.

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Interstitial cells in the vasculature

Cited by 71 publications

References 72 publications

Cajal-type cells from human mammary gland stroma: phenotype characteristics in cell culture

Cajal-type cells from human mammary gland stroma: phenotype characteristics in cell culture

Novel type of interstitial cell (Cajal-like) in human fallopian tube

Extrahepatic and Intrahepatic Human Portal Interstitial Cajal Cells

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