Interspecific Somatic Protoplast Fusion Products in Cultivated Jute Species

Kumar, Pavan; Das, Keshob Chandra; Sinha, R. R.; Mukherjee, Papiya; Sen, Sandeep

doi:10.1007/978-1-4684-4379-0_24

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…One of the major difficulties observed in jute protoplast isolation is the occurrence of high levels of mucilage in the source tissues. Similar observations were also made by Das et al (1981), Kumar et al (1983), and Islam (1987). As mucilage content is less in hypocotyls tissues, our experiments were continued with hypocotyl explants.…”

Section: Protoplast Isolationsupporting

confidence: 89%

“…Similar treatments were made by Khatun et al, (2002) who showed that temperature and growth conditions alter the production of certain compounds in the plant. Use of sea water for washing the explants was previously described by Kumar et al (1983). Among the three solutions (9% mannitol, CPW salt solution (Frearson et al, 1973) and 35% sea water) used for pelleting the protoplasts in our experiments, it was observed that 35% sea water was very effective as we could get good quantity of protoplasts.…”

Section: Protoplast Isolationmentioning

confidence: 77%

“…The only possible way to create hybrids of these species has been recognized as protoplast fusion since long (Das et al, 1981;Arangzeb & Khatun, 1983). Though there were studies on protoplast culture in cultivated jute species, none of them reported plant regeneration from protoplast derived cultures (Islam et al, 1981;Kumar et al, 1983;Saha & Sen, 1992;Khatun et al, 2002), the only exception being Saha et al (2001) who reported a limited success in obtaining embryo like structures from protoplasts. from hypocotyl explants.…”

Section: Introductionmentioning

confidence: 99%

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Studies on Explant Regeneration and Protoplast Culture from Hypocotyl Segments of Jute, Corchorus capsularis L.

Pushyami¹,

Beena²,

Sinha³

et al. 2012

JPS

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A repeatable protocol was developed for shoot regeneration from hypocotyl explants in jute (C. capsularis) that resulted in 25% regeneration using a combination of 6-BAP (7.5 mgl-1) and adenine hemisulfate (50 mgl-1). Rapid shoot proliferation was observed on medium containing NAA (0.1 mgl-1) and 6-BAP (1 mgl-1). The elongated shoots rooted on basal MS and could be successfully acclimatized, which later developed fertile flowers and viable seeds. After establishing regeneration protocol for jute from hypocotyl explants, the possibility of its response for protoplast isolation and culture was studied. The efficiency of isolation of protoplasts from hypocotyls was influenced by conditions of seedling growth, incubation time in enzyme solution, constitution of the enzyme mixture, osmoticum and purification methods. Isolated non-vacuolated protoplasts when cultured in modified Kao's medium with 7.2 gl-l sucrose produced microcalli in about four weeks. However, these microcalli did not develop further.

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Section: Protoplast Isolationsupporting

confidence: 89%

Section: Protoplast Isolationmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Studies on Explant Regeneration and Protoplast Culture from Hypocotyl Segments of Jute, Corchorus capsularis L.

Pushyami¹,

Beena²,

Sinha³

et al. 2012

JPS

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“…The other possible ways to improve the fiber crop are protoplast fusion of the two species having unique characters and genetic transformation [3][4][5][6][7][8] . Limited success in obtaining embryo like structures from protoplasts was reported 9 .…”

Section: Introductionmentioning

confidence: 99%

Multiple Shoot Regeneration from Excised Embryonic Axis Explants of Jute,Corchorus capsulariscv. JRC321

Joshi

Das

2021

Preprint

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Interspecific hybrids development by traditional breeding is challenging because of the incompatibility between two cultivated species of genus Corchorus. Under this condition, plant genetic transformation is an alternative tool for the improvement of jute cultivar. In the present study, in vitro multiple shoot regeneration protocol has been developed in jute (Corchorus capsularis cv. JRC 321) from excised embryonic axis as an explant by removing cotyledons, radicle, and tip of epicotyl. The explant is cultured in medium SMM1 supplemented with BAP (1.0 mg/L) for one week and sub-cultured in SMM6 containing IBA (0.5 mg/L) and BAP (0.2 mg/L). Direct adventitious shoot bud formation was observed from cotyledonary nodes and the adjacent region of excised embryonic axis. Interestingly, shoot bud formation was reduced when excised explants with intact epicotyl were cultured in the media combination (SMM1+SMM6) indicating the presence of apical dominance might be the cause of fewer shoot bud formation. While, hypocotyl explants did not produce any shoot in this media combination because nodal meristem was removed. However, the shoot buds developed from the excised embryonic axis were differentiated successfully into multiple shoots and showed further elongation in SMM6 which ultimately rooted in MS-B5 medium (+0.5 mg/L IBA). The plants were transferred successfully in the glasshouse conditions and appeared phenotypically normal.

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Somatic embryogenesis in protoplast derived calli of cultivated jute, Corchorus capsularis L.

Saha

Sen

1992

Plant Cell Reports

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Protoplasts were isolated from cotyledon, hypocotyl and mesophyll cells of Corchorus capsularis L., a major fibre crop, by one step enzyme digestion method. They were further cultured successfully on modified KM-8p (Kao and Michayluk, 1975) medium to form microcalli. The required cultural conditions could be used to achieve 34% to 78% plating efficiency, depending upon the source of protoplasts. Hypocotyl protoplasts gave the highest plating efficiency. On transfer to regeneration medium, somatic embryos developed at high frequency. The present success is a significant step forward in the development of meaningful plant cell culture methods for application in jute.

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Interspecific Somatic Protoplast Fusion Products in Cultivated Jute Species

Cited by 4 publications

References 17 publications

Studies on Explant Regeneration and Protoplast Culture from Hypocotyl Segments of Jute, Corchorus capsularis L.

Studies on Explant Regeneration and Protoplast Culture from Hypocotyl Segments of Jute, Corchorus capsularis L.

Multiple Shoot Regeneration from Excised Embryonic Axis Explants of Jute,Corchorus capsulariscv. JRC321

Somatic embryogenesis in protoplast derived calli of cultivated jute, Corchorus capsularis L.

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