Somatic embryogenesis in protoplast derived calli of cultivated jute, Corchorus capsularis L.

Saha, Tushar Kanti; Sen, Sandeep

doi:10.1007/bf00232386

Cited by 21 publications

(16 citation statements)

References 10 publications

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“…Protoplasts derived from cotyledonary tissue of C. capsularis cv. JRC 321' and hypocotyl protoplasts of C. olitorius cv.`JRO 878' were prepared following the method of Saha and Sen (1992). Fusions between anthocyanin-pigmented, red-coloured protoplasts derived from hypocotyls of`JRO 878' of C. olitorius, and green-coloured cotyledonary leaf protoplasts of C. capsularis cv.`JRC 321' were carried out.…”

Section: Methodsmentioning

confidence: 99%

Development of interspecific somatic hybrid cell lines in cultivated jute and their early characterization using jute chloroplast RFLP marker

Saha¹,

Majumdar

Banerjee

et al. 2001

Plant Breeding

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A strong sexual incompatibility barrier that exists between the two cultivated jute species, Corchorus capsularis and Corchorus olitorius, limits the scope for improvement through genetic introgression. Protoplast fusion was carried out to generate interspecific hybrid cell lines. Cotyledonary cell protoplasts of C. capsularis and anthocyaninpigmented hypocotyl protoplasts of C. olitorius were used in the fusion experiments, which appeared to be visually useful in the early selection of the fused products. A chloroplast DNA (cpDNA) marker was developed in jute, which showed species‐specific hybridization patterns with EcoRI‐digested total genomic DNA of C. capsularis and C. olitorius. This cpDNA marker was used in the characterization of the somatic hybrid cell lines at their early stages of growth. Evidence for the presence of both types of cpDNA in the hybrid cell lines was obtained when the total genomic DNA of 4‐ to 7‐month‐old hybrid cell lines was challenged with the chloroplast DNA marker through Southern analysis. It was shown that the early segregation of the parental chloroplasts did not occur in jute, although this is common in other plant species. The hybrid nature of the fused cell lines could also be identified through peroxidase isozyme analysis. Isozyme banding patterns were complex and varied among the hybrid cell lines.

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Section: Methodsmentioning

confidence: 99%

Development of interspecific somatic hybrid cell lines in cultivated jute and their early characterization using jute chloroplast RFLP marker

Saha¹,

Majumdar

Banerjee

et al. 2001

Plant Breeding

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“…There are reports of somatic embryos obtained from the protoplast derived calli of C. capsularis, which could not be established into plantlets. (Saha and Sen 1992). It was reported earlier that in vitro regeneration in this genus is genotype-dependent and the material is recalcitrant to regeneration .…”

Section: Introductionmentioning

confidence: 93%

In vitro regeneration and optimization of conditions for Agrobacterium mediated transformation in jute, Corchorus capsularis

Pushyami

Beena

Sinha

et al. 2011

J. Plant Biochem. Biotechnol.

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Jute is a crop of commercial importance that is widely cultivated for its bast fiber production but susceptible to many diseases that results in major economic loss. New genes can be introduced into this plant through Agrobacterium mediated genetic transformation for its genetic improvement, which is dependent on the availability of suitable in vitro techniques. An efficient regeneration system has been developed for in vitro culture of jute (Corchorus capsularis) from the distal cut ends of cotyledonary petioles. High frequency shoot regeneration was obtained on Murashige and Skoog (MS) nutrient agar medium supplemented with 0.5 mg/l NAA, 0.5 mg/l BAP and 36 g/l sucrose. On transfer to soil, the regenerated plantlets survived and appeared to be morphologically similar to the normal seed-grown plants. They developed pods and set fertile seeds. Histological analysis revealed de novo origin of shoot buds in the in vitro cultured cotyledonary petioles. Parameters affecting transformation were optimized by assaying GUS activity in these regenerable tissues after cocultivation with Agrobacteria. These tissues appear to be susceptible for infection and transformation by Agrobacterium carrying uid (GUS INT) and nptII genes, as well as shoot multiplication. The cells at the cut end of the petioles were found competent to take up the DNA, which was monitored by transient GUS gene expression. EHA105 at 0.3 O.D and LBA4404 at 0.5 O.D were found to be compatible in giving optimal levels of transient GUS expression.

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“…Attempts to obtain regeneration from hypocotyl and root calluses were made by subculturing on to basic MS medium supplemented with a range of concentrations of BA, zeatin and kinetin, with or without 0.1 and 0.5 mg 1-1 of the aux-ins IAA and NAA, and on the modified MS media of Saha and Sen (1992) and Gawal and Robacker (1990). Attempts to obtain regeneration from hypocotyl and root calluses were made by subculturing on to basic MS medium supplemented with a range of concentrations of BA, zeatin and kinetin, with or without 0.1 and 0.5 mg 1-1 of the aux-ins IAA and NAA, and on the modified MS media of Saha and Sen (1992) and Gawal and Robacker (1990).…”

Section: Regeneration Mediamentioning

confidence: 99%

“…Islam et al (1992) showed that protocols are available to obtain regenerants in some varieties C.capsularis, and Saha and Sen (1992) reported somat-ic embryogenesis from cotyledon and hypocotyl protoplast derived calluses in C.capsularis. Micropropagation would enable the rapid clonal multiplication of improved genotypes of this annual crop and the maintenance of useful genotypes.…”

Section: Introductionmentioning

confidence: 99%

Clonal propagation byin vitro culture of Corchorus (jute)

Abbas

Jones

Caligari

1997

Plant Cell Tiss Organ Cult

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Callus was produced on cotyledon, shoot tip, hypocotyl and root explants of two Corchorus species on several media. Cytokinin was necessary for callus production on cotyledon explants. Both C.olitorius genotypes produced most callus on media with zeatin and either NAA or IAA, and the C.capsularis genotype produced most callus on media with IAA and either zeatin or BA. High frequencies of regenerated shoots were obtained from shoot tip explants of both species, from the apical meristem and from callus. Media with 2.0 mg 1-1 BA were superior for both species, and media with zeatin were equally good for C.capsularis only. More regeneration was obtained for all genotypes after subculture of callus on media with 2.0 mg 1-I zeatin. Cotyledon callus produced less regeneration, also with differences between genotypes; explants of both genotypes of C.olitorius produced regeneration on a medium with NAA and zeatin, and the C.capsularis genotype produced regeneration on a medium with IAA and BA. Limited regeneration from root explant callus was obtained for C.capsularis only on medium with BA and IAA. Regeneration was not obtained from hypocotyl callus. Further regeneration of shoots of both species was obtained from secondary callus after subculture, and from nodal segments of regenerated shoots and of seedling shoots cultured on basic MS medium without growth hormones. Roots were produced on about 80% of all shoots after transference to medium with 0.2 mg 1 -l IBA, and rooted plantlets survived and flowered normally after transference to compost.Abbreviations: MS-Murashige and Skoog (1962); BA-6-benzylaminopurine; IAA-indole-3-acetic acid; IBAindole-3-butyric acid; NAA-naphthalene acetic acid

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Somatic embryogenesis in protoplast derived calli of cultivated jute, Corchorus capsularis L.

Cited by 21 publications

References 10 publications

Development of interspecific somatic hybrid cell lines in cultivated jute and their early characterization using jute chloroplast RFLP marker

Development of interspecific somatic hybrid cell lines in cultivated jute and their early characterization using jute chloroplast RFLP marker

In vitro regeneration and optimization of conditions for Agrobacterium mediated transformation in jute, Corchorus capsularis

Clonal propagation byin vitro culture of Corchorus (jute)

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