Interspecific cross between cucurbita pepo L. and Cucurbita martinezii through in vitro embryo culture

Metwally, E.; Haroun, Soliman A.; El-Fadly, G.

doi:10.1007/bf00025153

Cited by 19 publications

(4 citation statements)

References 7 publications

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“…Two wild squash species, C. lundelliana Bailey and C. okeechobeensis ssp. okeechobeensis Bailey, have been recently studied as useful sources for disease resistance such as powdery mildew and P. capsici crown rot resistance (Cohen et al, 2003;Contin and Munger, 1977;Metwally et al, 1996;. Cucurbita lundelliana is native to the Yucatan peninsula and can be hybridized with C. moschata, C. maxima, C. ficifolia, C. pepo, and C. argyrosperma (Ferriol and Pico ´, 2008;Sitterly, 1972;Whitaker, 1959).…”

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confidence: 99%

Screening of Cucurbita moschata Duchesne Germplasm for Crown Rot Resistance to Floridian Isolates of Phytophthora capsici Leonian

Chavez¹,

Kabelka²,

Chaparro³

2011

horts

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Phytophthora capsici causes seedling death, crown and root rot, fruit rot, and foliar blight on squash and pumpkins (Cucurbita spp. L.). A total of 119 C. moschata accessions, from 39 geographic locations throughout the world, and a highly susceptible butternut squash cultivar, Butterbush, were inoculated with a suspension of three highly virulent P. capsici isolates from Florida to identify resistance to crown rot. Mean disease rating (DR) of the C. moschata collection ranged from 1.4 to 5 (0 to 5 scale with 0 resistant and 5 susceptible). Potential resistant and tolerant individuals were identified in the C. moschata collection. A set of 18 PIs from the original screen were rescreened for crown rot resistance. This rescreen produced similar results as the original screen (r = 0.55, P = 0.01). The accessions PI 176531, PI 458740, PI 442266, PI 442262, and PI 634693 were identified with lowest rates of crown infection with a mean DR less than 1.0 and/or individuals with DR = 0. Further selections from these accessions could be made to develop Cucurbita breeding lines and cultivars with resistance to crown rot caused by P. capsici.

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confidence: 99%

Screening of Cucurbita moschata Duchesne Germplasm for Crown Rot Resistance to Floridian Isolates of Phytophthora capsici Leonian

Chavez¹,

Kabelka²,

Chaparro³

2011

horts

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“…One pioneering embryo rescue experiment conducted by Metwally et al involved wild Cucurbita martinezii Bailey and domesticated Cucurbita pepo L., utilizing MS media supplemented with 0.01 mg/L IAA and 0.1 mg/L KIN phytohormones. This approach yielded viable plants with morphological traits intermediate between the parents, exhibiting resistance to powdery mildew and cucumber mosaic virus [18]. Another study focusing on Chinese cucumber and the Japanese parental line Cucumis hystrix achieved a 37% regeneration rate using minimal hormonal intervention [46].…”

Section: Interspecific/intergeneric Hybridizationmentioning

confidence: 99%

“…Plants 2024, 13, 1320 2 of 13 Several studies have demonstrated the efficacy of embryo rescue in cucurbits, showcasing its potential in addressing breeding challenges and expanding the genetic diversity available for crop improvement [18][19][20]. For instance, Kaur et al successfully rescued embryos from crosses between different Cucumis species, producing fertile hull-less seed traits [21].…”

Section: Introductionmentioning

confidence: 99%

Widening Genetic Diversity Using Embryo Rescue in Cucurbit Crops: A Review

Reddy,

Ramireddy,

Reddy

2024

Plants

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Embryo rescue is a vital technique in cucurbit breeding and propagation, addressing challenges such as embryo abortion, poor seed viability, and incompatibility barriers. This method involves the excision of immature embryos from seeds followed by their in vitro culture on a nutrient medium, providing an environment conducive to their growth and development. In cucurbits, embryo rescue has been extensively utilized to overcome barriers to hybridization, enabling the production of interspecific and intergeneric hybrids with desired traits. Various factors, including genotype, developmental stage of embryos, and culture conditions, influence the success of embryo rescue in cucurbits. Optimal nutrient formulations, growth regulators, and culture techniques are critical for promoting embryo germination, shoot elongation, and subsequent plantlet establishment. Additionally, embryo rescue facilitates the recovery of valuable genetic material from wild and exotic cucurbit species, expanding genetic diversity and developing novel cultivars with improved traits such as disease resistance, yield, and quality. This review highlights the principles, applications, and advancements in embryo rescue technology in cucurbits, emphasizing its significance in cucurbit breeding programs and crop improvement efforts.

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“…Embryo rescue is an in vitro culture techniques used to assist the development of immature or weak embryos that might not survive to become viable plants. It has been used for developing interspecific hybrids between C. moschata , C. pepo, C. ficifolia and C. martinezii to widen the genetic base 17 , C. pepo and C. moschata for papaya ring spot virus resistance 18 and C. martinezii and C. pepo for powdery mildew and cucumber mosaic virus resistance 19 .…”

Section: Introductionmentioning

confidence: 99%

Interspecific hybridization for transfer of hull-less seed trait from Cucurbita pepo to C. moschata

Kaur

Garcha

Sandhu

et al. 2023

Sci Rep

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Hull-less seed trait is preferred by nut and oil industries worldwide for snacking and oil extraction as it evades the expensive decorticating (dehulling) process. This seed trait is available in C. pepo only, which has small seed cavity, sensitive to various biotic and abiotic stresses, and restricted to temperate regions for cultivation. Contrarily, the related species C. moschata has wider adaptability, disease tolerance and high seed yield. Therefore, attempt was made to transfer this trait into C. moschata through conventional pollination and ovule culture using four parents of hull-less C. pepo and six of hulled C. moschata. Through conventional approach, few viable F1 seeds (12–23) were obtained by using C. pepo as female parent, but in three crosses (HLP36 × HM1343, HLP36 × HM1022 and HLP44 × HM1022) only, whereas, its use as male parent was not successful. This incompatibility issue of reciprocals was resolved through ovule culture of C. moschata genotypes HM1343 and HM6711 after 17 to 19 days of pollination with C. pepo genotypes HLP53 and HLP72, respectively. The hybridity of interspecific crosses was confirmed through SSR markers (alleles inherited from both the parents), morphological characters and micromorphological leaf traits (differed from both the parents). The successful transfer through interspecific hybridization was further established with the presence of hull-less seed in fruits of F2 populations. Outcome of this study would pave the way for enhancing the productivity and multi-season cultivation of snack-seeded pumpkin even in subtropical and tropical regions.

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Interspecific cross between cucurbita pepo L. and Cucurbita martinezii through in vitro embryo culture

Cited by 19 publications

References 7 publications

Screening of Cucurbita moschata Duchesne Germplasm for Crown Rot Resistance to Floridian Isolates of Phytophthora capsici Leonian

Screening of Cucurbita moschata Duchesne Germplasm for Crown Rot Resistance to Floridian Isolates of Phytophthora capsici Leonian

Widening Genetic Diversity Using Embryo Rescue in Cucurbit Crops: A Review

Interspecific hybridization for transfer of hull-less seed trait from Cucurbita pepo to C. moschata

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