Interspecies spread of Staphylococcus aureus clones among companion animals and human close contacts in a veterinary teaching hospital. A cross-sectional study in Greece

Δρούγκα, Ελεάννα; Foka, Antigoni; Koutinas, Christos K.; Jelastopulu, Eleni; Giormezis, Nikolaos; Farmaki, Ourania; Sarrou, Styliani; Anastassiou, Evangelos D.; Petinaki, Efthimia; Spiliopoulou, Iris

doi:10.1016/j.prevetmed.2016.02.004

Cited by 33 publications

(31 citation statements)

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“…It was reported that sex and gender play an active role in the incidence of S. aureus bacteremia [30]. The detection rate of S. aureus in pets (17.1%) was higher than in previous studies from China [9,31], Zambia [32], Canada [33], and Australia [34], but lower than in the previous study from Greece [35]. The frequency of MRSA in veterinarians (9.8%) was higher in our study than in the previous study from China [9], but lower than in previous studies from Australia [11].…”

Section: Discussionmentioning

confidence: 60%

Biofilm Production Ability, Virulence and Antimicrobial Resistance Genes in Staphylococcus aureus from Various Veterinary Hospitals

et al. 2020

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Staphylococcus aureus (S. aureus) is one of the most clinically important zoonotic pathogens, but an understanding of the prevalence, biofilm formulation ability, virulence, and antimicrobial resistance genes of S. aureus from veterinary hospitals is lacking. By characterizing S. aureus in different origins of veterinary hospitals in Guangzhou, China, in 2019, we identified with the presence of S. aureus in pets (17.1%), veterinarians (31.7%), airborne dust (19.1%), environmental surfaces (4.3%), and medical device surfaces (10.8%). Multilocus sequence typing (MLST) and Staphylococcus protein A (spa) typing analyses demonstrated methicillin-sensitive S. aureus (MSSA) ST398-t571, MSSA ST188-t189, and methicillin-resistant S. aureus (MRSA) ST59-t437 were the most prevalent lineage. S. aureus with similar pulsed-field gel electrophoresis (PFGE) types distributed widely in different kinds of samples. The crystal violet straining assays revealed 100% (3/3) of MRSA ST59 and 81.8% (9/11) of MSSA ST188 showed strong biofilm formulation ability, whereas other STs (ST1, ST5, ST7, ST15, ST88, ST398, ST3154 and ST5353) showed weak biofilm production ability. Polymerase chain reaction (PCR) confirmed the most prevalent leucocidin, staphylococcal enterotoxins, ica operon, and adhesion genes were lukD-lukE (49.0%), sec-sel (15.7%), icaA-icaB-icaC-icaR (100.0%), and fnbB-cidA-fib-ebps-eno (100.0%), respectively. Our study showed that the isolates with strong biofilm production ability had a higher prevalence in clfA, clfB, fnbA and sdrC genes compared to the isolates with weak biofilm production ability. Furthermore, 2 ST1-MRSA isolates with tst gene and 1 ST88-MSSA isolate with lukS/F-PV gene were detected. In conclusion, the clonal dissemination of S. aureus of different origins in veterinary hospitals may have occurred; the biofilm production capacity of S. aureus is strongly correlated with ST types; some adhesion genes such as clfA, clfB, fnbA, and sdrC may pose an influence on biofilm production ability and the emergence of lukS/F-PV and tst genes in S. aureus from veterinary hospitals should raise our vigilance.

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Section: Discussionmentioning

confidence: 60%

Biofilm Production Ability, Virulence and Antimicrobial Resistance Genes in Staphylococcus aureus from Various Veterinary Hospitals

et al. 2020

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“…Transmission could occur by intensive contact sports (skin-to-skin, sharing equipment, and personal items) or via sports physicians and coaching staff [110]. Transmission of MRSA-ST80 among different host species in the context of households and veterinary practices has not been described, yet Drougka et al demonstrated that dogs and cats can be "vectors" of MRSA-ST80 PVL+ strains causing severe infections in humans [111].…”

Section: Mrsa-st80 Spread In the Communitymentioning

confidence: 99%

Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review

Mairi

Touati

Lavigne

2020

Toxins

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This review assessed the molecular characterization of the methicillin-resistant Staphylococcus aureus (MRSA)-ST80 clone with an emphasis on its proportion of total MRSA strains isolated, PVL production, spa-typing, antibiotic resistance, and virulence. A systematic review of the literature was conducted on MRSA-ST80 clone published between 1 January 2000 and 31 August 2019. Citations were chosen for a review of the full text if we found evidence that MRSA-ST80 clone was reported in the study. For each isolate, the country of isolation, the sampling period, the source of isolation (the type of infection, nasal swabs, or extra-human), the total number of MRSA strains isolated, number of MRSA-ST80 strains, antibiotic resistance patterns, PVL production, virulence genes, and spa type were recorded. The data from 103 articles were abstracted into an Excel database. Analysis of the data showed that the overall proportion of MRSA-ST80 has been decreasing in many countries in recent years. The majority of MRSA-ST80 were PVL positive with spa-type t044. Only six reports of MRSA-ST80 in extra-human niches were found. This review summarizes the rise of MRSA-ST80 and the evidence that suggests that it could be in decline in many countries.

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“…Multiple studies demonstrating the high prevalence of multidrug-resistant S. aureus, including ST398, among livestock in the European Union, Canada, and United States have been reported (Khanna, Friendship, Dewey, & Weese, 2008;Smith & Pearson, 2011). In Greece, even though S. aureus clones disseminated in the community and hospitals among humans are well characterized, limited data are available concerning clonal spread in food production chain (including animals, equipment, meat products, abattoir personnel) that may influence the epidemiology of staphylococcal infections in the community and hospital environments (Drougka et al, 2016(Drougka et al, , 2014(Drougka et al, , 2012Sarrou et al, 2015;Sergelidis et al, 2015).…”

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confidence: 99%

Multidrug‐resistant enterotoxigenicStaphylococcus aureuslineages isolated from animals, their carcasses, the personnel, and the environment of an abattoir in Greece

Δρούγκα

Foka

Giormezis

et al. 2019

J Food Process Preserv

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This study investigated genetic relatedness among Staphylococcus aureus from livestock, their carcass’ surfaces after slaughter, personnel, and equipment of a Greek abattoir. Genotyping was performed using PFGE, MLST, spa, and SCCmec. Antimicrobial susceptibility was determined according to EUCAST guidelines. Genes encoding staphylococcal enterotoxins (SEs) and Panton‐Valentine leukocidin (PVL) were detected by PCRs. From 392 samples, 46 S. aureus were recovered, 10 from workers, 6 from animals, 10 from carcass’, and 20 from equipment’s surfaces. Resistance to tetracycline was 80.4%, whereas, 58.7% of isolates were multi‐drug resistant. Eleven isolates (23.9%) were MRSA, 8 mecA‐ and 3 mecC‐positive. Fifteen PFGE and 13 spa types were identified, classified in 10 sequence types (STs). ST80 predominated among MSSA, and ST88‐IV clone among MRSA. Fifteen strains (32.6%) carried enterotoxin genes 7 of them possessed also the PVL genes. The abattoir seems to play important role in the transmission of S. aureus between animals and humans. Practical applications Antibiotic susceptibility, clonality, and toxin profiles among Staphylococcus aureus recovered from humans, animals, resulting carcasses and environmental surfaces of an abattoir were assessed. Our results showed spread of multi‐resistant toxigenic strains belonging to human and animals’ origin clones, indicating that the abattoir might be the reservoir for their transmission with potential negative impact on consumers’ health. The establishment and implementation of strict hygiene rules in the farms and during the slaughtering process in the abattoirs could control spread of virulent S. aureus strains.

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Interspecies spread of Staphylococcus aureus clones among companion animals and human close contacts in a veterinary teaching hospital. A cross-sectional study in Greece

Cited by 33 publications

References 32 publications

Biofilm Production Ability, Virulence and Antimicrobial Resistance Genes in Staphylococcus aureus from Various Veterinary Hospitals

Biofilm Production Ability, Virulence and Antimicrobial Resistance Genes in Staphylococcus aureus from Various Veterinary Hospitals

Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review

Multidrug‐resistant enterotoxigenicStaphylococcus aureuslineages isolated from animals, their carcasses, the personnel, and the environment of an abattoir in Greece

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