Interspecies interactions between Microcystis aeruginosa PCC 7806 and Desmodesmus subspicatus SAG 86.81 in a co-cultivation system at various growth phases

Omidi, Azam; Esterhuizen-Londt, Maranda; Pflugmacher, Stephan

doi:10.1016/j.envint.2019.105052

Cited by 15 publications

(10 citation statements)

References 55 publications

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“…When the diatom Thalassiosira pseudonana was co-cultivated with the bacteria Dinoroseobacter shibae at a 1:1 ratio, the metabolism of T. pseudonana was altered, but its overall growth rate was unchanged (Paul et al, 2013). When cyanobacteria Microcystis aeruginosa PCC 7806 and microalga Desmodesmus subspicatus were cocultivated in a designated dialysis tubing, the presence of M. aeruginosa did not influence the growth of the microalga at the early logarithmic growth phase, while the microalga started to out-compete the co-cultivated bacteria during the exponential phase of growth (Omidi et al, 2019). These findings indicate that species, cultivation conditions and co-cultivation ratio can all influence the results of co-cultivation.…”

Section: Growth (Cell Number Chlorophyll Dry Weight) and Paramylon mentioning

confidence: 98%

Global Metabolomics Reveals That Vibrio natriegens Enhances the Growth and Paramylon Synthesis of Euglena gracilis

Ouyang

Chen

Zhao

et al. 2021

Front. Bioeng. Biotechnol.

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The microalga Euglena gracilis is utilized in the food, medicinal, and supplement industries. However, its mass production is currently limited by its low production efficiency and high risk of microbial contamination. In this study, physiological and biochemical parameters of E. gracilis co-cultivated with the bacteria Vibrio natriegens were investigated. A previous study reports the benefits of E. gracilis and V. natriegens co-cultivation; however, no bacterium growth and molecular mechanisms were further investigated. Our results show that this co-cultivation positively increased total chlorophyll, microalgal growth, dry weight, and storage sugar paramylon content of E. gracilis compared to the pure culture without V. natriegens. This analysis represents the first comprehensive metabolomic study of microalgae-bacterial co-cultivation, with 339 metabolites identified. This co-cultivation system was shown to have synergistic metabolic interactions between microalgal and bacterial cells, with a significant increase in methyl carbamate, ectoine, choline, methyl N-methylanthranilate, gentiatibetine, 4R-aminopentanoic acid, and glu-val compared to the cultivation of E. gracilis alone. Taken together, these results fill significant gaps in the current understanding of microalgae-bacteria co-cultivation systems and provide novel insights into potential improvements for mass production and industrial applications of E. gracilis.

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Section: Growth (Cell Number Chlorophyll Dry Weight) and Paramylon mentioning

confidence: 98%

Global Metabolomics Reveals That Vibrio natriegens Enhances the Growth and Paramylon Synthesis of Euglena gracilis

Ouyang

Chen

Zhao

et al. 2021

Front. Bioeng. Biotechnol.

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“…The Microcystis cultures were exposed for 7 days to daphnid extracts as opposed to spent Daphnia medium which is often employed to explore the effects of zooplankton on microalgae. The limitation of spent grazer medium is that its chemical composition is difficult to standardize so as to be fully equivalent to cyanobacterial medium (utilized for control samples) and it can also contain allelopathic metabolites released by the microalgae with which cladocerans were fed (Harel et al, 2013;Omidi et al, 2019). The extract concentrations corresponded to a daphnid abundance of 5, 25, 50, and 100 individuals (ind)/L, which is within the range observed in eutrophic lakes (Saunders et al, 1999;Hülsmann and Voigt, 2002;Wojtal-Frankiewicz et al, 2015).…”

Section: Introductionmentioning

confidence: 66%

“…Firstly, the Microcystis cultures were exposed to homogenized extracts of cladocerans to avoid the exposure to chemical compounds that are unrelated directly to daphnid presence and which may alter the study results. For example, spent Daphnia medium, even though its nutrient levels can be standardized, may contain metabolites originating from microalgae used to feed daphnids which in turn can cause severe cell lysis in Microcystis (Harel et al, 2013;Omidi et al, 2019;Qiu et al, 2019). One should, however, note that in situ only a fraction of daphnid molecules is released and could operate as infochemicals for cyanobacteria by triggering the defense mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress, Programmed Cell Death and Microcystin Release in Microcystis aeruginosa in Response to Daphnia Grazers

et al. 2020

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There is increasing evidence that programmed cell death (PCD) in cyanobacteria is triggered by oxidative stress and that it contributes to the survival of the cyanobacterial population such as Microcystis aeruginosa. At the same time, microcystins (MCs) released during cell lysis have been implicated in colony formation (enabled by the release of polysaccharides) in M. aeruginosa-a strategy that allows the effect of a stressor, including grazing to be avoided or decreased. This experimental research has explored whether extracts of Daphnia magna and Daphnia cucullata (corresponding to 5, 25, 50, and 100 individuals per liter) reveal the effect on the growth, intracellular reactive oxygen species (ROS) content, lipid peroxidation, PCD, MC-LR release, and bound exopolysaccharide (EPS) level in M. aeruginosa during 7 days of exposure. As demonstrated, extracts of both daphnids induced dose-dependent growth inhibition, increase in ROS levels, lipid peroxidation, and PCD. Moreover, the release of MC-LR and an increase in the bound EPS fraction were observed in treated cultures. Generally, the greatest effects were observed under the influence of D. magna extracts. The study indicates that grazer presence can potentially trigger a series of events in the Microcystis population, with cells undergoing oxidative stress-induced PCD associated with MC release, which in turn increases EPS production by intact cells. As argued, this strategy is likely to have evolved in response to abiotic stressors, since both PCD and synthesis of MC in cyanobacteria predate the metazoan lineage. Nevertheless, it may still provide a benefit for the survival of the MC-producing M. aeruginosa population under grazer pressure.

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“…From the current literature, pure toxins, toxin containing crude extracts, filtrates of single and mixed cultures as well as the intact cells of the toxin producers induced different physiological and metabolic responses [ 25 , 94 , 219 ]. Another essential consideration for intraspecies interaction studies is the contribution of other secondary metabolites in addition to cyanotoxins.…”

Section: Discussionmentioning

confidence: 99%

“…It is important to repeat such experiments using log phase grown cells since there are many indications that those respond differently to biotic and abiotic conditions [ 93 ]. The stationary phase growth of D. subspicatus was also inhibited in co-cultivation with the MC-producing M. aeruginosa PCC 7806 and when exposed to the spent medium of Microcystis [ 94 ]. Filtrates from toxic M. aeruginosa induced colony formation in C. vulgaris , subject to the initial density of the green alga and the growth phase of M. aeruginosa , and with continuous field exposure inhibited the growth of the green alga [ 95 ].…”

Section: Cyanotoxins and Interspecies Interplaymentioning

confidence: 99%

Reviewing Interspecies Interactions as a Driving Force Affecting the Community Structure in Lakes via Cyanotoxins

et al. 2021

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The escalating occurrence of toxic cyanobacterial blooms worldwide is a matter of concern. Global warming and eutrophication play a major role in the regularity of cyanobacterial blooms, which has noticeably shifted towards the predomination of toxic populations. Therefore, understanding the effects of cyanobacterial toxins in aquatic ecosystems and their advantages to the producers are of growing interest. In this paper, the current literature is critically reviewed to provide further insights into the ecological contribution of cyanotoxins in the variation of the lake community diversity and structure through interspecies interplay. The most commonly detected and studied cyanobacterial toxins, namely the microcystins, anatoxins, saxitoxins, cylindrospermopsins and β-N-methylamino-L-alanine, and their ecotoxicity on various trophic levels are discussed. This work addresses the environmental characterization of pure toxins, toxin-containing crude extracts and filtrates of single and mixed cultures in interspecies interactions by inducing different physiological and metabolic responses. More data on these interactions under natural conditions and laboratory-based studies using direct co-cultivation approaches will provide more substantial information on the consequences of cyanotoxins in the natural ecosystem. This review is beneficial for understanding cyanotoxin-mediated interspecies interactions, developing bloom mitigation technologies and robustly assessing the hazards posed by toxin-producing cyanobacteria to humans and other organisms.

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Interspecies interactions between Microcystis aeruginosa PCC 7806 and Desmodesmus subspicatus SAG 86.81 in a co-cultivation system at various growth phases

Cited by 15 publications

References 55 publications

Global Metabolomics Reveals That Vibrio natriegens Enhances the Growth and Paramylon Synthesis of Euglena gracilis

Global Metabolomics Reveals That Vibrio natriegens Enhances the Growth and Paramylon Synthesis of Euglena gracilis

Oxidative Stress, Programmed Cell Death and Microcystin Release in Microcystis aeruginosa in Response to Daphnia Grazers

Reviewing Interspecies Interactions as a Driving Force Affecting the Community Structure in Lakes via Cyanotoxins

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