Interrogation of individual intratumoral B lymphocytes from lung cancer patients for molecular target discovery

Campa, Michael J.; Moody, M. Anthony; Zhang, Ruijun; Liao, Hua‐Xin; Gottlin, Elizabeth B.; Patz, Edward F.

doi:10.1007/s00262-015-1787-0

Cited by 19 publications

(11 citation statements)

References 49 publications

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“…16,17 Importantly, the B cell chemoattractant CXCL13, secreted by tumor cells, follicular dendritic cells, and T follicular helper cells in human lung tumor lesions, was shown to be responsible for the influx of B cells into the tumor. 16,18,19 Upon entering the local microenvironment, tumor antigens released from lung cancer cells aid in B cell aggregation and trigger B cellmediated antigen presentation, which facilitate the maintenance of B cell activation and proliferation. 19,20 Immunohistochemistry (IHC) and flow cytometric analysis show that all major subsets of B cells can be found within TIB populations (Table 1).…”

Section: The Infiltration Location and Maintenance Of Tibs In Humanmentioning

confidence: 99%

“…16,18,19 Upon entering the local microenvironment, tumor antigens released from lung cancer cells aid in B cell aggregation and trigger B cellmediated antigen presentation, which facilitate the maintenance of B cell activation and proliferation. 19,20 Immunohistochemistry (IHC) and flow cytometric analysis show that all major subsets of B cells can be found within TIB populations (Table 1). 21 TIBs, including CD20 + B cells and CD138 + plasma cells, primarily localize to lymphoid aggregates within the lung tumor stroma, the typical model being termed a tertiary lymphoid structure (TLS).…”

Section: The Infiltration Location and Maintenance Of Tibs In Humanmentioning

confidence: 99%

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Tumor-infiltrating B cells: their role and application in anti-tumor immunity in lung cancer

et al. 2018

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Evidence indicates that lung cancer development is a complex process that involves interactions between tumor cells, stromal fibroblasts, and immune cells. Tumor-infiltrating immune cells play a significant role in the promotion or inhibition of tumor growth. As an integral component of the tumor microenvironment, tumor-infiltrating B lymphocytes (TIBs) exist in all stages of cancer and play important roles in shaping tumor development. Here, we review recent clinical and preclinical studies that outline the role of TIBs in lung cancer development, assess their prognostic significance, and explore the potential benefit of B cell-based immunotherapy for lung cancer treatment.

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Section: The Infiltration Location and Maintenance Of Tibs In Humanmentioning

confidence: 99%

Section: The Infiltration Location and Maintenance Of Tibs In Humanmentioning

confidence: 99%

Tumor-infiltrating B cells: their role and application in anti-tumor immunity in lung cancer

et al. 2018

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“…In a recent study exploring tumor-infiltrating lymphocytes, all of the isolated single B cells produced monoclonal antibodies that were polyreactive. 2 Thus, although a patient's serum may appear positive for a given autoantigen, the signal could be due to antibodies that also react to proteins unrelated to the tumor. If possible, it may be necessary to design the test such that polyreactivity of antibodies is minimized.…”

Section: Discussionmentioning

confidence: 99%

“…Details of this procedure have been described previously. 2 For autoantigen identification, we probed twodimensional polyacrylamide gel electrophoresis blots of the appropriate antigen source with each immunoreactive serum. We then used the location of the immunoreactive spot as a guide to locate the protein of interest on a duplicate Coomassie-stained two-dimensional polyacrylamide gel electrophoresis gel of the same sample.…”

Section: Methodsmentioning

confidence: 99%

Rethinking Autoantibody Signature Panels for Cancer Diagnosis

Campa

Gottlin

Herndon

et al. 2017

Journal of Thoracic Oncology

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“…The functions of B lymphocytes are adjusted by a number of signaling pathways, some of which involve the B-cell receptor (BCR) ( 8 ). Syk exhibits a central role in the activation of the BCR ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

In�vitro and in�vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis

et al. 2018

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Chronic glomerulonephritis (CGN) is the most common form of glomerular disease; however, its associated molecular mechanisms remain unclear. Spleen tyrosine kinase (Syk) is a key mediator of B-receptor signaling on the surface of inflammatory cells. The primary target for R406 is Syk. The aim of the present study was to investigate the molecular mechanisms involved in a rat model of CGN induced by adriamycin (ADR) and in the rat glomerular mesangial cell line, HBZY-1, stimulated by lipopolysaccharide (LPS). CGN was induced in the rat models by two intravenous injections of ADR into the tail: 3.5 mg/kg ADR was given on the first day and 3.0 mg/kg on the fourteenth day. HBZY-1 cells were incubated with 0.5 µg/ml LPS for 48 h. The pathological alterations in the kidney tissues were observed by hematoxylin and eosin staining. The 24 h urinary protein, blood urea nitrogen (BUN) and creatinine levels were measured using an automatic biochemistry analyzer. The mRNA expression levels of Syk, Ras, mitogen activated protein kinase kinase (MEK), extracellular signal regulated kinase (ERK)1/2 and c-Fos was measured by reverse transcription-quantitative polymerase chain reaction. Subsequently, the protein levels of phosphorylated (p)-Syk, Ras, p-MEK1/2, p-ERK1/2 and c-Fos were measured by western blot analysis. In the model group, 24 h urinary protein, BUN and creatinine levels were increased when compared with the normal group (P<0.05). In addition, compared with the normal group, the mRNA and protein levels of the Syk/Ras/c-Fos pathway components in vitro and in vivo were markedly increased, inhibiting the abnormal cell viability of mesangial cells. In conclusion, the results of the present study suggested a potential role for the Syk/Ras/c-Fos signaling pathway in CGN, which indicated the necessity for further investigation at the clinical level.

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Interrogation of individual intratumoral B lymphocytes from lung cancer patients for molecular target discovery

Cited by 19 publications

References 49 publications

Tumor-infiltrating B cells: their role and application in anti-tumor immunity in lung cancer

Tumor-infiltrating B cells: their role and application in anti-tumor immunity in lung cancer

Rethinking Autoantibody Signature Panels for Cancer Diagnosis

In�vitro and in�vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis

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