Interrelationships Between Follicular Fluid Steroid Levels, GonadotropiC Stimuli, and Oocyte Maturation During Preovulatory Development of Porcine Follicles1

Ainsworth, L.; Tsang, Benjamin K.; Downey, B.R.; Marcus, G. J.; Armstrong, David T.

doi:10.1095/biolreprod23.3.621

Cited by 144 publications

(91 citation statements)

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“…Our follicle diameter and steroid levels in FF agree quite well with recently published data with well-defined porcine material (Corbin et al 2003) or earlier data (Ainsworth et al 1980, Babalola & Shapiro 1988, Grant et al 1989. The periovulatory group is defined and selected by the very high ascent of PGF2a level in FF.…”

Section: Follicle Groupsupporting

confidence: 89%

Expression and possible role of fibroblast growth factor family members in porcine antral follicles during final maturation

Schams

Steinberg²,

Šteffl³

et al. 2009

Reproduction

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The aim of this study was to investigate the possible participation of fibroblast growth factor (FGF) family members (FGF1, FGF2 and FGF7 and their receptors) in porcine follicles (polyovulatory species) under special consideration for FGF2 during final growth. A classification of follicles was done by size and follicular fluid content of oestradiol-17b, progesterone and prostaglandin F2a. The mRNA expression of examined factors was analysed by real-time PCR. The hormone concentration was estimated by enzyme immunoassay, protein characterisation by western blotting and localisation by immunohistochemistry. Follicle tissue separated in theca interna and granulosa cells was extracted and tested for mRNA of FGF1, FGF2, FGF7 and receptors (FGFR1IIIc, FGFRIIIb and FGFR2IIIc). Additionally, the mRNA expression of FSHR, LHR and aromatase cytochrome P450 for further characterisation of follicles was analysed. Significantly, higher FGF2 protein levels were measured in stroma when compared with total follicle or corpus luteum tissue. This result was confirmed by western blot with two strong bands. Immunological localisation of FGF2 only in stroma (fibroblasts) confirms the protein measurements. The results show a clear difference for FGF2 protein expression during final growth of follicles if monovulatory (bovine) and polyovulatory (porcine) species are compared. FGF2 protein in porcine ovary may be (due to localisation and concentration in stroma) important for support of angiogenesis of more follicles (polyovulatory species) and not of a single follicle like in cows.

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Section: Follicle Groupsupporting

confidence: 89%

Expression and possible role of fibroblast growth factor family members in porcine antral follicles during final maturation

Schams

Steinberg²,

Šteffl³

et al. 2009

Reproduction

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“…This result is comparable to those of Ainsworth et al (1980;P 4 : 901 ± 208 ng/ml, E 2 : 14.4 ± 1.6 pg/ml) and Blödow et al (1990;P 4 : 675.1 ± 135.4 ng/ml, E 2 : 20.8 ± 14.2 pg/ml). Regardless of whether they are from PMSG/hCG-primed or spontaneously ovulating gilts, late preovulatory follicles have a higher progesterone/oestradiol-17β ratio (Ainsworth et al, 1980;Blödow et al, 1990;Guthrie et al, 1993;Conley et al, 1994). This is due to the dramatic decrease of follicular capacity to produce oestradiol-17β, and the increased progesterone production after preovulatory LH surge or hCG administration (Downey and Driancourt, 1994).…”

Section: Discussionsupporting

confidence: 89%

“…It has been demonstrated that follicles are heterogeneous both in spontaneously ovulating and PMSG/hCG-primed gilts and sows. Morphological and biochemical asynchrony continues into the immediate preovulatory period and follicles respond differently to the LH/hCG surge signal, reflecting their maturational status at that time (Ainsworth et al, 1980;Grant, 1989;Hunter et al, 1989;Hunter and Wiesak, 1990).…”

Section: Discussionmentioning

confidence: 99%

Follicular and oocyte development in gilts of different age

Brüssow

Rátky²,

Torner

et al. 2002

Acta Veterinaria Hungarica

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The aim of the present study was to estimate follicular and oocyte development of the same gilts in three phases of their reproductive life -prepuberal gilt (6 months old), cycling gilt (9.5 months old) and primiparous sow. Follicular development was induced by injections of 1000 IU PMSG followed by 500 IU hCG 72 h later. Cumulus-oocyte-complexes (COCs) were recovered from preovulatory follicles of the left ovary, and follicular fluid (FF) from the right ovary always 34 h after hCG by endoscopy. Altogether, 19 gilts were used in the prepuberal (P) and cycling (C) trials and 12 of them in the primiparous trial (S). Altogether 168, 190 and 82 follicles were aspirated from the left ovary and 106, 125 and 42 COCs recovered (recovery rate 60.5 ± 26.9, 62.7 ± 20.9 and 52.9 ± 21.8%). The average number of follicles was higher in C compared to P (19.7 ± 6.8 vs. 15.7 ± 6.8, p = 0.06) and to S (14.2 ± 4.0, p < 0.05), respectively. More uniform expanded COCs were aspirated from prepuberal and cycling gilts as compared to sows (89.7 and 78.4% vs. 46.3%, p < 0.05). Furthermore, the meiotic configuration in oocytes differed (p < 0.05) between these groups (55.5 and 61.7% vs. 0% Telo1/Meta2). Concentrations of progesterone in FF decreased (p < 0.05) from 590.0 ± 333.6 (P) to 249.1 ± 72.6 (C) and 161.4 ± 75.2 ng/ml (S). FF concentrations of oestradiol-17β were different between gilts and sows (9.3 ± 2.9, 21.9 ± 10.6 and 94.0 ± 15.9 pg/ml, p < 0.05). The progesterone/oestradiol ratio was 72.1, 15.2 and 4.7. Results indicate a different follicular and oocyte development during the investigated lifetime periods. Cycling gilts should preferably be used in IVF and breeding programs. The lower reproductive potential of primiparous sows is taken into consideration at breeding. Prediction of lifetime performance based on individual ovarian reaction of prepuberal gilts is unsuitable.

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“…Levels of PGE 2 in FF were observed to peak at around 36 h post-hCG in the follicle (Tsang et al 1979). An inverse relationship of progesterone to oestrogen was observed at the time of PGE 2 peak (Ainsworth et al 1980) and the theca cells were found to be the predominant follicular source of PGE 2 compared to the granulosa cells (Evans et al 1983). These observations, together with our previous finding of upregulation of endometrial COX-2 by SP, support the proposal that uterine-derived PGE 2 may be the active intermediary in accelerating the follicular events, which culminate in ovulation followed by corpus luteum development.…”

Section: S O'leary and Othersmentioning

confidence: 95%

“…The role of prostaglandins in preovulatory follicular function has been described in prepubertal gilts synchronised with eCG and hCG (Tsang et al 1979, Ainsworth et al 1980, Evans et al 1983. Levels of PGE 2 in FF were observed to peak at around 36 h post-hCG in the follicle (Tsang et al 1979).…”

Section: S O'leary and Othersmentioning

confidence: 99%

Seminal plasma regulates ovarian progesterone production, leukocyte recruitment and follicular cell responses in the pig

O’Leary

Jasper²,

Robertson³

et al. 2006

Reproduction

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Seminal plasma (SP) acts to influence the uterine endometrium after mating, activating synthesis of embryotrophic cytokines and inflammatory changes that condition the tract for embryo implantation and establishing pregnancy. The objective of this study was to investigate in pigs whether the ovary might also be responsive to SP exposure. Prepubertal gilts were synchronised with exogenous gonadotrophins and received transcervical treatment with pooled boar SP or PBS; then the ovarian tissue was recovered at 34 h (preovulation) and on days 5 and 9 after treatment. The ovarian response was assessed by measuring ovulation rate, number and size of corpora lutea, ovarian leukocyte populations, progesterone production in vivo, as well as responses of retrieved granulosa cells cultured in vitro. In SP-treated gilts, leukocyte recruitment into the ovarian tissues was increased fourfold at 34 h, with macrophages comprising the most abundant cell lineage. There was no effect of SP on the number of oocytes ovulated; however, the weight of corpora lutea was increased in SP-treated gilts. SP also induced an increase in plasma progesterone content seen from day 5 to at least day 9 after treatment. In addition, granulosa cells and thecal tissue retrieved from preovulatory follicles of SP-treated gilts were more responsive in vitro to growth factor-and gonadotrophin-stimulated cell proliferation and progesterone synthesis. These results suggest that uterine exposure to SP influences immune cell trafficking in the ovary and enhances steroidogenesis in early pregnancy. The effects of SP on ovarian function potentially contribute to reproductive success in the pig.

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Interrelationships Between Follicular Fluid Steroid Levels, GonadotropiC Stimuli, and Oocyte Maturation During Preovulatory Development of Porcine Follicles1

Abstract: Prepubertal gifts were treated with 750 IU pregnant mare serum gonadotropin (PMSG) and 72 h later with 500 IU human chonionic gonadotropin (hCG)

Cited by 144 publications

References 0 publications

Expression and possible role of fibroblast growth factor family members in porcine antral follicles during final maturation

Expression and possible role of fibroblast growth factor family members in porcine antral follicles during final maturation

Follicular and oocyte development in gilts of different age

Seminal plasma regulates ovarian progesterone production, leukocyte recruitment and follicular cell responses in the pig

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