Interrelationship between extracellular enzyme activity, ATP content, total counts of bacteria and CO2 evolution

Asmar, Farouq; Eiland, F.; Nielsen, Niels Erik

doi:10.1007/bf00395465

Cited by 46 publications

(11 citation statements)

References 20 publications

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“…The results of the correlation analyses were also similar to those by the application of glucose in the incubation tests (Nannipieri et al 1979(Nannipieri et al , 1983Asmar et al 1992), and field tests (Kandeler et al 1994;Badalucco et al 1996) and different from those in paddy field soils (Watanabe andHayano 1995, 1996a). Compared to our former results on paddy field soils, it was difficult to differentiate clearly the bacteria involved in the process of proteolysis (soil protease production) from the bacteria involved in the subsequent ammonification process, and those involved in the subsequent nitrification process, based on the correlation analyses.…”

Section: Discussionsupporting

confidence: 60%

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Changes in Soil Protease Activity and Numbers of Culturable Bacteria in Upland Fields by the Application of Liquid Livestock Feces

Watanabe

Niimi

2005

Soil Science and Plant Nutrition

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Within 1 to 3 weeks after the application of liquid livestock feces, the soil benzyloxycarbonyl‐l‐phenylalanyl‐l‐leucinase (z‐FLase) and caseinase activities in upland fields (120 t feces ha−1 year−1, 600 t feces ha−1 year−1) increased to the maximum levels (z‐FLase 562 pKat and 840 pKat g−1 dry soil, respectively; caseinase 1,018 pKat and 2,141 pKat g−1 dry soil, respectively), and decreased to the original level after 5 weeks. Numbers of culturable bacteria counted on albumin medium (AA), bacteria counted on peptone‐polymixin medium (PP), and vegetative cells of Bacillus spp. counted on BTV medium (BTV) also increased to the maximum levels after application of the feces. Although there was a significantly high correlation between bacterial number on AA and both the soil protease activities (z‐FLase, r=0.895; caseinase, r=0.935), more significant correlations were observed between bacterial number on AA and inorganic nitrogen contents (NH4+ content, r=0.902; NO3− content, r=0.981). Similarly, although a significant correlation was observed between the soil caseinase activity and bacterial number on PP (r=0.835) and BTV (r=0.780), and the number of fluorescent Pseudomonas spp. (P1) (r=0.867), more significant correlations were observed between the numbers of these culturable bacteria and the soil NH4+ content (PP, r=0.944; BTV, r=0.959; P1, r=0.933) and soil NO3− content (PP, r=0.982; BTV, r=0.961; P1, r=0.990). Therefore it was difficult to identify the bacterial group responsible for the soil protease production based on these correlation analyses. Rapid increase in the total bacterial number (AA) by the 600 t application was mainly due to the increased number of bacteria on PP medium, which were assumed to have been introduced from liquid livestock feces.

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Section: Discussionsupporting

confidence: 60%

“…Changes in the soil protease activities by the application of feces were similar to those by the application of glucose in the incubation tests (Nannipieri et al 1979(Nannipieri et al , 1983Asmar et al 1992Asmar et al , 1994 and different from those in paddy field soils (Watanabe and Hayano 1995).…”

Section: Discussionmentioning

confidence: 62%

Changes in Soil Protease Activity and Numbers of Culturable Bacteria in Upland Fields by the Application of Liquid Livestock Feces

Watanabe

Niimi

2005

Soil Science and Plant Nutrition

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“…Soil phosphatase activity accompanied with soil urease activity can be a useful compound index for soil fertility (Qiu et al 2004). Soil catalase activity is sensitive to soil biological factors (Asmar et al 1992), is closely related with soil major nutrient elements (Rodriguez-Kabana and Truelove 1982), and inhibited by soil contamination of Pb and Cd Xi (i = 1-3) stands for soil OM, TN, TP (see Table 1) respectively; (0.15 level, n = 50) (Liu et al 2007). All reports mentioned above mainly concerned about soil contamination, and the knowledge on enzymes activities related to soil fertility in urbanized anthropogenic soils is still scarce.…”

Section: Catalase Activitymentioning

confidence: 99%

Enzyme activities of urban soils under different land use in the Shenzhen city, China

Shi¹,

Lu²,

Xu³

et al. 2008

Plant Soil Environ.

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Urbanization has drastically changed soil properties, and an assessment of these changes is essential for soil management and soil health. The activities of urease, acid phosphatase, invertase and catalase, soil organic matter, pH, electrical conductivity (EC) and clay (< 0.01 mm) content of urban soils under two land-uses in the central built-up area of the Shenzhen city were investigated, and multivariate analysis was used to study the relationship between soil enzymes and soil physical-chemical properties. The results showed that invertase activity in roadside soil was significantly higher than that in urban park soil, whereas catalase activity was significantly higher in urban park soil. Soil organic matter had significant positive correlation with activities of invertase, urease and acid phosphatase but not with catalase. Soil pH had a significant negative direct effect on urease and acid phosphatase activity, but the effect was counteracted by positive indirect effect of soil organic matter. Soil EC had a positive direct effect on activities of catalase and there was a significant correlation between soil EC and soil catalase activities. Soil organic matter, soil pH and EC were the major factors influencing activities of soil enzymes.

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“…Thus, the intercept and the slope of the regression line may be affected by the composition of the microbial populations as well as by physical and chemical properties in soil. Asmar et al (1992) and Watanabe and Hayano (1996) found that extractable enzyme activity increased more drastically than did total activity but it only refers to a small amount of the enzyme present in soil (Tabatabai, 1994). However, both total and extractable enzyme activities were correlated after substrate addition ( Asmar et al, 1992).…”

Section: Extracellular Urease Activitymentioning

confidence: 99%

“…Asmar et al (1992) and Watanabe and Hayano (1996) found that extractable enzyme activity increased more drastically than did total activity but it only refers to a small amount of the enzyme present in soil (Tabatabai, 1994). However, both total and extractable enzyme activities were correlated after substrate addition ( Asmar et al, 1992). Furthermore, ATP content and protease activity were correlated after substrate addition ( Asmar et al, 1992); the same occurred for values measured during composting of organic wastes ( Garcia et al, 1992).…”

Section: Extracellular Urease Activitymentioning

confidence: 99%

Intracellular and extracellular enzyme activity in soil with reference to elemental cycling

Duly

Nannipieri

1998

J Plant Nutrition & Soil

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Enzyme activities play an important role for the transformation of elements and compounds in soil and, thus, were extensively analyzed for more than 4 decades. The activity of any enzyme in soil may not only be controlled by active organisms. Substantial parts of ‘extracellular’ enzymes may be stabilized by abiotic soil components maintaining their activity. Methods to discriminate the source of enzyme activity were summarized with emphasis on the approach plotting enzyme activity versus a feature integrating the microbial biomass after the addition of glucose and nitrate. Considering the quotient between enzyme activity and microbial biomass content, protease activity will be discussed with reference to nitrogen transformation in soils.

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Interrelationship between extracellular enzyme activity, ATP content, total counts of bacteria and CO2 evolution

Cited by 46 publications

References 20 publications

Changes in Soil Protease Activity and Numbers of Culturable Bacteria in Upland Fields by the Application of Liquid Livestock Feces

Changes in Soil Protease Activity and Numbers of Culturable Bacteria in Upland Fields by the Application of Liquid Livestock Feces

Enzyme activities of urban soils under different land use in the Shenzhen city, China

Intracellular and extracellular enzyme activity in soil with reference to elemental cycling

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