During the adaptation of plants to low temperature, changes in gene expression can be induced in a variety of tissues. Lowtemperature-regulated gene expression was studied in cold-stored potato (Solanum tuberosum 1.) tubers by two-dimensional electrophoresis of in vitro translation products. As a response to cold treatment, the relative amount of mRNA encoding at least 26 polypeptides changed. By differential screening of a cDNA library, 16 clones corresponding to cold-inducible transcripts were isolated. lhey were classified into four non-cross-hybridizing groups. RNA hybridizations using representative clones from each group revealed different temporal accumulation patterns for the coldinducible transcripts. mRNAs homologous to the cDNA clones were first detectable after 1 to 3 d of cold treatment, and the highest level of expression was reached after 3 to 7 d. Transcripts corresponding to cDNA clones C113 and C119 were transiently expressed, whereas the steady-state level remained high for cDNA clones C17 and Cl2l during the cold storage period of 4 weeks. lhe DNA sequences of two cDNA clones, C17 and C119, have been determined. lhe polypeptide predicted from the DNA sequence of C119 is sequence related to small heat-shock proteins from other plant species. lhe deduced protein sequence of C17 exhibits strong homology to the dehydrin/RAB group of dehydration stress-and abscisic acid-inducible polypeptides and to cold-induced proteins from Arabidopsis and spinach.Higher or lower than optimal temperatures exert stress on plants and induce changes in cellular metabolism leading to adaptation (Sachs and Ho, 1986;Guy, 1990). Severa1 temperate plants increase their freezing tolerance ("cold hardening") when exposed to low, nonfreezing temperatures. In storage organs of many such species, sugars accumulate in response to the cold treatment, a phenomenon known for a long time as "cold sweetening" (Miiller-Thurgau, 1882). Mechanisms and regulation of the biochemical processes leading to cold sweetening are in large part still unclear. What is known is that chilling alters the lipid composition of the cell membranes and increases the enzymic activity of specific steps in different metabolic pathways related to carbohydrate and amino acid metabolism (Guy, 1990;Thomashow, 1990 Sachs and Ho, 1986).The number of studies describing cold-induced variations in mRNA populations is steadily increasing (reviewed by Guy, 1990, andThomashow, 1990). Moreover, a number of cDNA clones encoding cold-regulated transcripts have been isolated from aerial tissue of alfalfa, Arabidopsis, barley, spinach, tomato, and wheat (for review see Cattivelli and Bartels, 1992, and refs. therein). Among the few cold-induced genes for which a possible function has been proposed is C14 of tomato, a gene encoding a protein closely related to thiol proteases (Schaffer and Fischer, 1988).Low-temperature-regulated gene expression in cold-stored potato (Solanum tuberosum L.) tubers is the subject of this paper. Cold-induced metabolic changes leading to the ...