Interpopulation peroxidase variability in<i>Sphagnum girgensohnii</i>Russ.

Krzakowa, Maria; Klama, Henryk

doi:10.1179/jbr.1992.17.1.27

Cited by 4 publications

(5 citation statements)

References 3 publications

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“…A correlation was also found between the amplification patterns of genomic DNA with katG sequences and electrophoretic peroxidase patterns in the genus Calamagrostis [43] and Lolium [44]. Peroxidases proved to be effective species-specific markers for the genus Sphagnum [45,46], which additionally supported the use of the katG gene sequence in this study.…”

Section: Introductionsupporting

confidence: 56%

“…In addition to the above peat moss species, those enzymes supported the molecular identification of four species of the section Subsecunda: S. contortum, S. denticulatum, S. inundatum and S. subsecundum [11]. Characteristic patterns of cathodic peroxidase bands were also determined for S. girgensohnii [45] and S. russowii [46]. Those enzymes also supported the identification of S. capillifolium and S. rubellum [6] with a high degree of probability.…”

Section: Discussionmentioning

confidence: 86%

“…In the group of 15 species-specific katG bands, the highest number was observed in S. fimbriatum and S. capillifolium (5 each), while no marker bands were determined for S. girgensohnii which could point to a high degree of peroxidase variability in this species [45]. katG sequences were also effectively used as species-specific markers and section markers in a study of the genera Polygonatum [20] and Aneura [21].…”

Section: Discussionmentioning

confidence: 99%

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A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

Sawicki

Szczecińska

2011

Acta Soc Bot Pol

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IntroductionDue to their great phenotypic plasticity, the classification of peat moss species often requires microscopic identification, which is both labor-and time-consuming. In addition to a standard morphological description, the classification of bryophytes increasingly often involves the use of molecular markers [1,2]. Chloroplast and nuclear genomes are mainly used, but this approach implies high analysis costs. Species-specific molecular markers support material identification even when diagnostic traits cannot be observed. The above applies mostly to traits related to sexual reproduction (gametengia, sporophytes) and vegetative reproduction (gemmae) which are often undeveloped or already degraded.The identification of peat moss species possess some problems. According to various authors, their number ranges from 39 [3] to 340 [4]. The main reason for such a wide variation in the reported number of Sphagnum species is their high phenotypic plasticity. Mosses growing in wet habitats exhibit high morphological variability in response to water level fluctuations and changes in other environmental conditions [5]. Another factor that affects morphological variability and hinders the species classification of peat mosses is hybridization [6][7][8][9].Isoenzymatic electrophoresis is a rapid and relatively inexpensive method of molecular species identification. The use of markers of this class permitted the molecular identification of the majority of morphologically controversial species of the genus Sphagnum [10,11]. However, their practical application is difficult, as in order to obtain stable and distinct isoenzymatic patterns, plants should be grown under glass-house conditions to normalize their expression levels and increase vitality [12]. Electrophoresis of enzymatic proteins cannot be applied to herbaceous materials, either, which makes it useless as a tool for verifying the correctness of species identification. Therefore, other molecular markers enabling a rapid, reliable and inexpensive identification have to be found.PCR-based DNA analyses provided new insights into the taxonomy of peat mosses. Studies of the genus Sphagnum rely primarily on RAPD [13,14], ISSR [9,15] and SSR [16] markers, and ITS sequences [17,18]. With a few exceptions [19], the above markers have not been used for molecular species identification.The objective of the present study was to evaluate the suitability of five types of DNA markers for molecular species identification. Peat moss species of the section Acutifolia were analyzed. Apart from markers that are commonly applied in studies of the genus Sphagnum, i.e. RAPD, ISSR and nuclear ITS regions, we also tested ISJ markers and primers complementary to the katG gene, which had been successfully used as species-specific markers in higher plants [20] The nuclear ITS region, analyzed based on the polymorphism of restriction loci [24,25] and a direct comparison of sequences [18,26], is widely used in the taxonomic studies of bryophytes. An extensive review of ITS applications i...

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Section: Introductionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

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A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

Sawicki

Szczecińska

2011

Acta Soc Bot Pol

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“…Sphagnum girgensohnii has so far been reported with a chromosome number of n519, and S. russowii n538, for their gametophores (Fritsch, 1991). Krzakowa, Klama & Wojtuń (1992) report results indicating absence of diploid races of S. girgensohnii in Polish material. The triploid capsules may thus be considered F 1 sporophyte hybrids, and represent a case of spontaneous hybridization across ploidy levels in bryophytes.…”

Section: Discussionmentioning

confidence: 89%

Interploidal gene flow and introgression in bryophytes:Sphagnum girgensohnii×S. russowii, a case of spontaneous neotriploidy

Flatberg¹,

Thingsgaard²,

Såstad³

2006

Journal of Bryology

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Hybrid zones between female sporophyte-bearing haploid Sphagnum girgensohnii and male allodiploid S. russowii were studied. The existence of hybrids was initially hypothesized based on incidence of conspicuously large sporophytes in S. girgensohnii in the presence of male S. russowii. Measurements of nuclear DNA content, morphology and isozymes provide the first evidence of spontaneous hybridization across ploidal levels in bryophytes. Viable spores from the hybrid capsules yielded triploid protonemata and juvenile gametophores in culture. Isozyme profiles of the triploid gametophores displayed unbalanced heterozygous patterns containing two alleles. As S. russowii is an allodiploid species with S. girgensohnii and S. rubellum as progenitors, isozyme banding patterns of the triploids are interpreted as consisting of a single allele copy from the S. rubellum genome in S. russowii, and two copies of another allele at the same locus inherited from S. girgensohnii. The triploid sporophytes can be considered as F 2 hybrids formed by introgression. The allotriploid gametophores are formed through diplospory or syndiploidy in the hybrid capsules. Comparison of hybrid sporophyte morphology to S. girgensohnii and S. russowii confirmed that hybrid capsules are larger and with an abnormally swollen sporophyte foot. The spore sizes of the hybrid capsules were significantly larger than spores from normal, intraspecific S. girgensohnii capsules. The percentage of germinated spores was ,5% in cultures from hybrid sporophytes, which is much lower than in intraspecific sporophytes. The new knowledge opens the way for re-evaluation of polyploid evolution in bryophytes with emphasis on gametic non-reduction, introgression and two-step models of polyploid formation.

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“…Affinities among the taxa within the studied group were assessed by coefficient of taxonomic similarity S [7]. Values of coefficient S for each pair-wise comparison among the taxa were calculated separately for each set of seed proteins (anodal, cathodal, SDS-PAGE).…”

Section: Methodsmentioning

confidence: 99%

Systematic Relationships Among Six Taxa of Genus Elymus as Revealed by Electrophoresis of Seed Proteins

Angelov¹,

Bednarska²

2018

VLUBS

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of seed proteins. Affinities among the taxa were assessed by coefficient of taxonomic similarity S. An index of group affinity (GA) was also calculated for each taxon as a sum of its S values. The results indicated that most of studied taxa are nearly equidistantly positioned within the examined group of genus Elymus. The species E. pycnanthus proved to be most distantly positioned within the group as its index GA (1.08) was the lowest one. The species E. dahuricus (GA=1.21) was also comparatively distant within the studied group of genus Elymus. It should be noted that the hybrid E. x mucronatus is more close positioned to E. repens than to the other parent E. hispidus as judged by the composition of its seed proteins. The results of the present study clearly showed that within mixed populations of E. repens, E. hispidus and E. ×mucronatus well developed hybrid swarms can be observed.

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Interpopulation peroxidase variability inSphagnum girgensohniiRuss.

Cited by 4 publications

References 3 publications

A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

Interploidal gene flow and introgression in bryophytes:Sphagnum girgensohnii×S. russowii, a case of spontaneous neotriploidy

Systematic Relationships Among Six Taxa of Genus Elymus as Revealed by Electrophoresis of Seed Proteins

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