Interplay between potato virus X and RNA granules in Nicotiana benthamiana

Robles-Luna, Gabriel; Furman, Nicolás; Barbarich, María Florencia; Carlotto, Nicolás; Attorresi, Alejandra; García, María Laura; Kobayashi, Ken

doi:10.1016/j.virusres.2019.197823

Cited by 7 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…found that eukaryotic translation initiation factor 4 gamma1(eIF4GI) interacts with G3BP to promote typical SG formation, whereas picornaviruses 2A or L can block typical SG formation by disrupting the eIF4GI‐G3BP interaction (Yang et al., 2019). In the present study, the abundance of NtG3BPL1‐derived granule‐like aggregates was decreased by ChiVMV infection (Figure 5a), which is consistent with the previous findings that hypoxia‐induced AtUBP1b cytoplasmic granules are inhibited upon potato virus X (PVX) infection (Robles‐Luna et al., 2020). Recently, Hoffmann et al.…”

Section: Discussionsupporting

confidence: 93%

NtG3BPL1 confers resistance to chilli veinal mottle virus through promoting the degradation of 6K2 in tobacco

Peng,

Jiao,

Cheng

et al. 2024

The Plant Journal

View full text Add to dashboard Cite

SUMMARYThe RNA regulatory network is a complex and dynamic regulation in plant cells involved in mRNA modification, translation, and degradation. Ras‐GAP SH3 domain‐binding protein (G3BP) is a scaffold protein for the assembly of stress granules (SGs) and is considered an antiviral component in mammals. However, the function of G3BP during virus infection in plants is still largely unknown. In this study, four members of the G3BP‐like proteins (NtG3BPLs) were identified in Nicotiana tabacum and the expression levels of NtG3BPL1 were upregulated during chilli veinal mottle virus (ChiVMV) infection. NtG3BPL1 was localized in the nucleus and cytoplasm, forming cytoplasmic granules under transient high‐temperature treatment, whereas the abundance of cytoplasmic granules was decreased under ChiVMV infection. Overexpression of NtG3BPL1 inhibited ChiVMV infection and delayed the onset of symptoms, whereas knockout of NtG3BPL1 promoted ChiVMV infection. In addition, NtG3BPL1 directly interacted with ChiVMV 6K2 protein, whereas 6K2 protein had no effect on NtG3BPL1‐derived cytoplasmic granules. Further studies revealed that the expression of NtG3BPL1 reduced the chloroplast localization of 6K2‐GFP and the NtG3BPL1‐6K2 interaction complex was localized in the cytoplasm. Furthermore, NtG3BPL1 promoted the degradation of 6K2 through autophagy pathway, and the accumulation of 6K2 and ChiVMV was affected by autophagy activation or inhibition in plants. Taken together, our results demonstrate that NtG3BPL1 plays a positive role in tobacco resistance against ChiVMV infection, revealing a novel mechanism of plant G3BP in antiviral strategy.

show abstract

Section: Discussionsupporting

confidence: 93%

NtG3BPL1 confers resistance to chilli veinal mottle virus through promoting the degradation of 6K2 in tobacco

Peng,

Jiao,

Cheng

et al. 2024

The Plant Journal

View full text Add to dashboard Cite

show abstract

“…However, these functions relative to the phenotypes that regulate their formation remain an active area of research and is often contested [65][66][67][68][69]. Interestingly, the formation of both SG and P-bodies are also induced during viral infection by a diverse array of viruses [70][71][72][73][74]. A growing number of studies have begun to find a range of anti-viral roles for RNA granules during viral infection, many of which often link back to the translational arrest or degradation of viral RNA at these sites.…”

Section: Shiftless and Rna Granules During Rna Virus Infectionmentioning

confidence: 99%

Shiftless, a Critical Piece of the Innate Immune Response to Viral Infection

Rodríguez

Muller

2022

Viruses

View full text Add to dashboard Cite

Since its initial characterization in 2016, the interferon stimulated gene Shiftless (SHFL) has proven to be a critical piece of the innate immune response to viral infection. SHFL expression stringently restricts the replication of multiple DNA, RNA, and retroviruses with an extraordinary diversity of mechanisms that differ from one virus to the next. These inhibitory strategies include the negative regulation of viral RNA stability, translation, and even the manipulation of RNA granule formation during viral infection. Even more surprisingly, SHFL is the first human protein found to directly inhibit the activity of the -1 programmed ribosomal frameshift, a translation recoding strategy utilized across nearly all domains of life and several human viruses. Recent literature has shown that SHFL expression also significantly impacts viral pathogenesis in mouse models, highlighting its in vivo efficacy. To help reconcile the many mechanisms by which SHFL restricts viral replication, we provide here a comprehensive review of this complex ISG, its influence over viral RNA fate, and the implications of its functions on the virus-host arms race for control of the cell.

show abstract

“…However, these functions relative to the phenotypes that regulate their formation remain an active area of research and is often contested [68][69][70][71][72]. Interestingly, the formation of both SGs and P-bodies are also induced during viral infection by a diverse array of viruses [73][74][75][76][77]. A growing number of studies have begun to find a range of antiviral roles for RNA granules during viral infection, many of which often link back to the translational arrest or degradation of viral RNA at these sites.…”

Section: Shiftless and Rna Granules During Rna Virus Infectionmentioning

confidence: 99%

Shiftless, A Critical Piece of the Innate Immune Response to Viral Infection

Rodríguez¹,

Muller²

2022

Preprint

View full text Add to dashboard Cite

Since its initial characterization in 2016, the interferon stimulated gene Shiftless (SHFL) has proven to be a critical piece of the innate immune response to viral infection. SHFL expression stringently restricts the replication of multiple DNA, RNA, and retroviruses with an extraordinary diversity of mechanisms that differ from one virus to the next. These inhibitory strategies include the negative regulation of viral RNA stability, translation, and even the manipulation of RNA granule formation during viral infection. Even more surprisingly, SHFL is the first human protein found to directly inhibit the activity of the -1 programmed ribosomal frameshift, a translation recoding strategy utilized across nearly all domains of life and a several human viruses. Recent literature has shown that SHFL expression also significantly impacts viral pathogenesis in mouse models, highlighting its in-vivo efficacy. To help reconcile the many mechanisms by which SHFL restricts viral replication, we provide here a comprehensive review of this complex ISG, its influence over viral RNA fate, and the implications of its functions on the virus-host arms race for control of the cell.

show abstract

Interplay between potato virus X and RNA granules in Nicotiana benthamiana

Cited by 7 publications

References 37 publications

NtG3BPL1 confers resistance to chilli veinal mottle virus through promoting the degradation of 6K2 in tobacco

NtG3BPL1 confers resistance to chilli veinal mottle virus through promoting the degradation of 6K2 in tobacco

Shiftless, a Critical Piece of the Innate Immune Response to Viral Infection

Shiftless, A Critical Piece of the Innate Immune Response to Viral Infection

Contact Info

Product

Resources

About