Interpenetrating polymer network (IPN) scaffolds of sodium hyaluronate and sodium alginate for chondrocyte culture

Chung, Chung-Wook; Kang, Jeong Yeon; Yoon, In‐Soo; Hwang, Hyung-Don; Balakrishnan, Pachamuthu; Cho, Hyun‐Jong; Chung, Kyudon; Kang, Dae-Hwan; Kim, Dae‐Duk

doi:10.1016/j.colsurfb.2011.08.005

Cited by 42 publications

(24 citation statements)

References 34 publications

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“…Semi-quantitative histopathological grading was performed using a modified version of the Chambers scoring system [32, 33], which has been established by the OARSI histopathology initiative as the standard method for grading mouse cartilage degeneration [34]. Based on this system, paraffin sections from each sample were scored after safranin-O staining.…”

Section: Methodsmentioning

confidence: 99%

Photo-crosslinked HAMA hydrogel with cordycepin encapsulated chitosan microspheres for osteoarthritis treatment

Chen

Mei

et al. 2016

Oncotarget

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Autophagy is a protective mechanism in normal cartilage. The present study aimed to investigate the synergistic therapeutic effect of promotion of chondrocyte autophagy via exposure to cordycepin encapsulated by chitosan microspheres (CM-cordycepin) and photo-crosslinked hyaluronic acid methacrylate (HAMA) hydrogel, with the goal of evaluating CM-cordycepin as a treatment for patients with osteoarthritis. First, we developed and evaluated the characteristics of HAMA hydrogels and chitosan microspheres. Next, we measured the effect of cordycepin on cartilage matrix degradation induced by IL1-β in chondrocytes and an ex vivo model. Cordycepin protects cartilage from degradation partly by activation of autophagy. Moreover, we surgically induced osteoarthritis in mice, which were injected intra-articularly with CM-cordycepin and HAMA. The combination of CM-cordycepin and HAMA hydrogel retarded the progression of surgically induced OA. Cordycepin ameliorated cartilage matrix degradation at least partially by inducing autophagy in vivo. Our results demonstrate that the combination of cordycepin encapsulated by CMs and photo-crosslinked HAMA hydrogel could be a promising strategy for treating patients with osteoarthritis.

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Section: Methodsmentioning

confidence: 99%

Photo-crosslinked HAMA hydrogel with cordycepin encapsulated chitosan microspheres for osteoarthritis treatment

Chen

Mei

et al. 2016

Oncotarget

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“…Due to its biological and structural importance, as well as its ease of modification, HA is an attractive polymer for biomedical applications [17]. Since the turnover of HA is rapid, about one-third of the total HA body content is degraded and reformed daily, covalent crosslinking is necessary to increase mechanical stability for tissue engineering purposes [14, 18, 19]. This can be accomplished either directly or by first modifying the hydroxyl or carboxyl groups of HA with functional moieties, which can then be crosslinked [15].…”

Section: Introductionmentioning

confidence: 99%

Enzymatically crosslinked silk-hyaluronic acid hydrogels

et al. 2017

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In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues. The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization. Utilizing the same method of crosslinking, tyramine-substituted HA forms hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly. To address the limitations of these singular component scaffolds, HA was covalently crosslinked with silk, forming a composite hydrogel that exhibited both mechanical integrity and hydrophilicity. The composite hydrogels were assessed using unconfined compression and infrared spectroscopy to reveal of the physical properties over time in relation to polymer concentration. In addition, the hydrogels were characterized by enzymatic degradation and for cytotoxicity. Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization. These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.

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“…The composite gel on the other hand showed an elastic modulus of ~6 kPa, showing the possibility of increasing the elastic modulus of injectable hyaluronic acid gels by the addition of alginic acid. Similarly, using interpenetrating polymer network scaffolds of sodium hyaluronate and sodium alginate, Chung et al showed the feasibility of increasing the strength of hyaluronic acid scaffold by the addition of sodium alginate [32]. The incorporation of hyaluronic acid has been demonstrated to change the structure of ionically cross-linked alginate gels, thereby modifying the composite gel properties [46].…”

Section: Resultsmentioning

confidence: 99%

“…Different cross-linking techniques have been investigated to develop hyaluronic acid gels with varying mechanical properties as scaffolds for cartilage tissue engineering [30]. However, the rapid degradation and clearance of the gel may raise limitations in supporting long term tissue regeneration [32]. Clearly, there exists a need to develop improved hydrogel systems that combine the advantages of these natural polymers to serve as cartilage and osteochondral scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Enzymatically cross-linked alginic–hyaluronic acid composite hydrogels as cell delivery vehicles

Ganesh

Hanna

Nair

et al. 2013

International Journal of Biological Macromolecules

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An injectable composite gel was developed from alginic and hyaluronic acid. The ezymatically cross-linked injectable gels were prepared via the oxidative coupling of tyramine modified sodium algiante and sodium hyaluronate in the presence of horse radish peroxidase (HRP) and hydrogen peroxide (H2O2). The composite gels were prepared by mixing equal parts of the two tryaminated polymer solutions in 10U HRP and treating with 1.0% H2O2. The properties of the alginate gels were significanly affected by the addition of hyaluronic acid. The percentage water absorption and storage modulus of the composite gels were found to be lower than the alginate gels. The alginate and composite gels showed lower protein release compared to hyaluronate gels in the absence of hyaluronidase. Even hyaluronate gels showed only approximately 10% protein release after 14 days incubation in phosphate buffer solution. ATDC-5 cells encapsulated in the injectable gels showed high cell viability. The composite gels showed the presence of enlarged spherical cells with significantly higher metabolic activity compared to cells in hyaluronic and alginic acid gels. The results suggest the potential of the composite approach to develop covalently cross-linked hydrogels with tuneable physical, mechanical, and biological properties.

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Interpenetrating polymer network (IPN) scaffolds of sodium hyaluronate and sodium alginate for chondrocyte culture

Cited by 42 publications

References 34 publications

Photo-crosslinked HAMA hydrogel with cordycepin encapsulated chitosan microspheres for osteoarthritis treatment

Photo-crosslinked HAMA hydrogel with cordycepin encapsulated chitosan microspheres for osteoarthritis treatment

Enzymatically crosslinked silk-hyaluronic acid hydrogels

Enzymatically cross-linked alginic–hyaluronic acid composite hydrogels as cell delivery vehicles

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