Photo-crosslinked HAMA hydrogel with cordycepin encapsulated chitosan microspheres for osteoarthritis treatment

Chen, Xia; Chen, Pengfei; Mei, Sheng; Ning, Lei; Lei, Chenyang; Wang, Jiying; Zhang, Jianfeng; Ma, Jianjun; Fan, Shunwu

doi:10.18632/oncotarget.13748

Cited by 40 publications

(36 citation statements)

References 39 publications

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“…This material is modified in half of the papers, obtaining: hyaluronic acid methacrylated (HAMA) (Costantini et al, 2016 ; Mouser et al, 2017 ), acrylated-HA and tyramine-conjugated HA (Lee et al, 2018 ), dopamine-conjugated HA (Haring et al, 2019 ), and thiol functionalized HA (Stichler et al, 2017 ). Concretely, HAMA provides great tunability for specific uses at different methacrylation degrees (Xia et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Sánchez¹,

Gómez-Blanco²,

Nieto

et al. 2020

Front. Bioeng. Biotechnol.

119

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Nowadays, bioprinting is rapidly evolving and hydrogels are a key component for its success. In this sense, synthesis of hydrogels, as well as bioprinting process, and cross-linking of bioinks represent different challenges for the scientific community. A set of unified criteria and a common framework are missing, so multidisciplinary research teams might not efficiently share the advances and limitations of bioprinting. Although multiple combinations of materials and proportions have been used for several applications, it is still unclear the relationship between good printability of hydrogels and better medical/clinical behavior of bioprinted structures. For this reason, a PRISMA methodology was conducted in this review. Thus, 1,774 papers were retrieved from PUBMED, WOS, and SCOPUS databases. After selection, 118 papers were analyzed to extract information about materials, hydrogel synthesis, bioprinting process, and tests performed on bioprinted structures. The aim of this systematic review is to analyze materials used and their influence on the bioprinting parameters that ultimately generate tridimensional structures. Furthermore, a comparison of mechanical and cellular behavior of those bioprinted structures is presented. Finally, some conclusions and recommendations are exposed to improve reproducibility and facilitate a fair comparison of results.

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Section: Resultsmentioning

confidence: 99%

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Sánchez¹,

Gómez-Blanco²,

Nieto

et al. 2020

Front. Bioeng. Biotechnol.

119

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“…For immunohistochemical examination, the sections were treated with 0.3% H 2 O 2 for 10 min, blocked with normal horse serum for 30 min, and incubated in the presence of the primary antibody (Bridgewater et al, ; Fujishiro, Kaneko, Kawashima, Ishida, & Kawano, ; Gu et al, ; Hwang et al, ; Tu et al, ; Xia et al, ; Yeom et al, ) (Table ) at 4°C overnight. To reduce the non‐specific reactions, we diluted secondary antibody twice times with normal horse serum (ImmPRESS HRP REAGENT KIT, Vector Laboratories, Burlingame, CA), and the sections were incubated in the diluted solution for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Histomorphometry of ectopic mineralization using undecalcified frozen bone sections

Fujihara

Chiba

Nakagawa

et al. 2018

Microscopy Res & Technique

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To investigate the correlation between mineral formation and enhanced expressions of some proteins using undecalcified frozen bone sections. Histological studies have revealed that some proteins, such as BMP2, BMPR1A, and Connexin 43, are expressed in and around sites of ectopic ossification. However, the relationship between the expressed proteins considered to be associated with the ossification and mineral formation in vivo is not clear. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1)-mutant spinal hyperostotic TWY mice and ICR mice as controls were euthanized after calcein labeling, and undecalcified frozen sections were obtained from the middle thoracic spine. Intervertebral disc areas were examined histologically and by measuring calcein-labeled areas and areas showing immunoreactivity for BMP2, BMPR1A, and Connexin 43. Calcein-labeled areas, indicating mineralization in the ectopic mineralization sites, were significantly larger in the mutant mice than in controls. The expression of Connexin 43 was elevated in the annulus fibrosus. Increases in the calcein-labeled areas was not correlated with increases in the areas showing immunoreactivity for Connexin 43 in the annulus fibrosus. There was no statistical correlation between enhanced immunohistochemical expression and elevated calcein-labeled areas. By applying the morphometrical analysis method using undecalcified frozen sections to ENPP1-mutant mice, quantitative evaluation of the mineralization and proteins expressed in the surrounding area in the same animal became possible. K E Y W O R D S BMP2, BMPR1A, Connexin 43, ENPP1, histomorphometry, mineralization, ossification of the posterior longitudinal ligament, undecalcified frozen section

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“…In the OA group, all the samples were classified as grade 3 while those in the control group were all grade 0. Human cartilage samples were obtained using previously described methods [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

Sanguinarine protects against osteoarthritis by suppressing the expression of catabolic proteases

Sun

Huang

et al. 2017

Oncotarget

Self Cite

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Inflammatory cytokines play critical roles in the pathogenesis of osteoarthritis. Recent studies have demonstrated that natural active substances can serve as alternative therapeutic agents for the prevention and treatment of osteoarthritis. Sanguinarine, an alkaloid isolated from the roots of Sanguinaria canadensis, is known to have anti-inflammatory properties. The aim of the present study was to investigate the therapeutic effect of Sanguinarine against osteoarthritis. Sanguinarine inhibited interleukin-1β-induced expression of matrix metalloproteinase 1, 3, and 13, and A disintegrin and metalloproteinase with thrombospondin motifs-5 in chondrocytes, which involved the nuclear factor-κB and c-Jun N-terminal kinase signalling pathways. Furthermore, the study of interleukin-1β-induced cartilage matrix degradation in an anterior cruciate ligament transection-induced osteoarthritis model revealed that Sanguinarine ameliorated osteoarthritis by inhibiting the expression of matrix metalloproteinase 1, 3, and 13, and A disintegrin and metalloproteinase with thrombospondin motifs-5. In conclusion, we demonstrated for the first time that Sanguinarine suppressed the expression of matrix metalloproteinase 1, 3, and 13, and A disintegrin and metalloproteinase with thrombospondin motifs-5 in vitro, ex vivo, and in vivo, indicating its potential usefulness in treating osteoarthritis.

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Photo-crosslinked HAMA hydrogel with cordycepin encapsulated chitosan microspheres for osteoarthritis treatment

Cited by 40 publications

References 39 publications

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Histomorphometry of ectopic mineralization using undecalcified frozen bone sections

Sanguinarine protects against osteoarthritis by suppressing the expression of catabolic proteases

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