Interpenetrating Low-Molecular Weight Hyaluronic Acid in Hyaluronic Acid-Based In Situ Hydrogel Scaffold for Periodontal and Oral Wound Applications

Abstract

Tissues engineering has gained a lot of interest, since this approach has potential to restore lost tooth-supporting structures, which is one of the biggest challenges for periodontal treatment. In this study, we aimed to develop an in situ hydrogel that could conceivably support and promote the regeneration of lost periodontal tissues. The hydrogel was fabricated from methacrylated hyaluronic acid (MeHA). Fragment/short-chain hyaluronic acid (sHA) was incorporated in this hydrogel to encourage the bio-synergistic effects of two different molecular weights of hyaluronic acid. The physical properties of the hydrogel system, including gelation time, mechanical profile, swelling and degrading behavior, etc., were tested to assess the effect of incorporated sHA. Additionally, the biological properties of the hydrogels were performed in both in vitro and in vivo models. The results revealed that sHA slightly interfered with some behaviors of networking systems; however, the overall properties were not significantly changed compared to the base MeHA hydrogel. In addition, all hydrogel formulations were found to be compatible with oral tissues in both in vitro and in vivo models. Therefore, this HA-based hydrogel could be a promising delivery system for low molecular weight macromolecules. Further, this approach could be translated into the clinical applications for dental tissue regeneration.

Statistical optimization of hydrazone-crosslinked hyaluronic acid hydrogels for protein delivery

“…Due to the rapid nature of hydrazone crosslinking between HA-Ox and HA-ADH, in which bonds form too rapidly to measure the gelation point using rheology, the gelation time of each hydrogel was determined using the inverted tube test, which measures the point at which the hydrogel reaches a fully gelled state. [45][46][47] 50 mL each of HA-ADH and HA-Ox were pipetted into 2 mL microcentrifuge tubes to create 100 mL hydrogels and inverted every minute until gelation occurred. The time at which there was no viscous flow was recorded as the gelation time.…”

Recent Advances in Functional Hydrogels for Treating Dental Hard Tissue and Endodontic Diseases

“…(Figure ). With the continuous development and improvement of the functionalization of hydrogels, more and more hydrogels are used in basic and clinical oral research, including periodontal treatment, − surgical treatment (e.g., oral cancer, wound healing after tooth extraction), − mucosal diseases (e.g., oral ulcers, stomatitis), − and dental hard tissue/endodontic diseases (tooth whitening, oral caries, root canal treatment, etc. ). − Next, we will focus on the application of hydrogels in the treatment of several typical dental hard tissue and endodontic diseases, give a detailed analysis, and make a summary.…”

Hydrogels for Oral Tissue Engineering: Challenges and Opportunities

“…HA, another important component of the ECM of connective tissues and periodontal ligament matrix, has valuable potential in periodontal tissue regeneration [116][117][118][119].…”

“…In a clinical study, the efficacy of a biohydrogel containing recombinant human fibroblast growth factor type 2 (rhFGF-2) in a hyaluronic acid (HA) vehicle was evaluated for treating periodontal bone intraosseous defects. The study included thirty adult patients, with the control group being treated with papilla-preserved flaps for open debridement, and the trial group applying rhFGF- HA, another important component of the ECM of connective tissues and periodontal ligament matrix, has valuable potential in periodontal tissue regeneration [116][117][118][119].…”