Interobserver agreement for estrogen receptor immunohistochemical analysis in breast cancer: a comparison of manual and computer-assisted scoring methods

Diaz, Leslie K.; Şahin, Ayşegül; Sneige, Nour

doi:10.1016/j.anndiagpath.2003.11.004

Cited by 52 publications

(63 citation statements)

References 15 publications

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“…While ER and PR scores were assessed; only nuclear expression was concerned (Lester et al, 2009;Hammond et al, 2010) and for ER staining of >10% of tumor nuclei was considered positive. PR expression was considered to be positive if the nuclei of more than 1% of cells were stained positive (Diaz et al, 2004;Fisher et al, 2005). HER2 score assessment was made according to intensities and the proportions of the cells which showed membrane staining (Lester et al, 2009;Gutierrez and Schiff, 2011).…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Diagnostic and Prognostic Importance of the Neutrophil Lymphocyte Ratio in Breast Cancer

Özyalvaçlı¹,

Yesil²,

Karğı³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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IntroductionsBreast cancer is the most common type of cancer in women and the risk increases with age. As in other tumors the development of breast carcinoma is associated with systemic inflammation. The systemic inflammatory response can change with tumor growth, invasion, angiogenesis and metastasis. İnflammatory markers such as c reactive protein (CRP) and interleukin 6 (IL 6) are increased in breast cancer patients and a have adverse effect on survey (Balta et al., 2013).Benign proliferative breast disease (BPBD) is most common in women who are in their thirties to fifties. Various sizes of cysts, adenosis, hyperplasia with atypia and/or without atypia and apocrine metaplasia can be seen in BPBD on microscopic examination. Despite the strong association between BPBD and breast cancer development, the exact causes of BPBD are still unknown. Hormonal imbalance especially increased estrogen via to progestin is thought to play a role in development of BPBD. Breast cancer risk related with proliferative breast disease. Several studies showed that inflammation plays an important role in the development of BPBD and early breast carcinogenesis (Catsburg et al., 2014).Complete blood count and its subtype neutrophil count and neutrophil lymphocyte ratio (NLR) are an indicator of systemic inflammation (Guthrie et al., 2013). AbstractBackground: The aim of this study was to determine diagnostic and prognostic roles of the neutrophil to lymphocyte ratio (NLR) in breast cancer patients. To date, data are limited on associations of primary breast carcinoma (PBC) and benign proliferative breast disease (BPBD) with preoperative NLR values. Materials and Methods: Our study covered of 120 female patients with PBC and 50 with BPBD. Diagnostic values of NLR were estimated using sensitivity, specificity and areas under receiver operating characteristic curves (AUC). Results: NLR values were significantly higher in the PBC patients than in those with BPBD, with an AUC of 0.668 in the PBC case. The optimal cut-off for NLR was 2.96 and this was validated in the testing set, giving a sensitivity and a specificity of 79.7% and 76.2%, respectively, in PBC patients. Conclusions: Preoperative high NLR is a significant diagnostic predictor of distinction of breast cancer from BPBD and elevated NLR is also an important prognostic marker for primary invasive breast cancer.

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Section: Immunohistochemical Stainingmentioning

confidence: 99%

Diagnostic and Prognostic Importance of the Neutrophil Lymphocyte Ratio in Breast Cancer

Özyalvaçlı¹,

Yesil²,

Karğı³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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“…With regard to a cut-off value for Er evaluation by computerized image analysis, 10% of positive cells (9,16,20), the h-score (15) and aQua scores calculated by the average signal intensity divided by compartment area (18) were used in previous reports. although in the present study the allred score determined by human examiners was compared to the h-score calculated by the computer and the allred/h-score conversion table was shown in a previous report (26), it was difficult to translate one scoring system to the other system, precisely since the two systems are not strictly equivalent.…”

Section: Discussionmentioning

confidence: 99%

“…in particular, automated image analysis technology, including aQua (18), ariol (14,19) and matlab7 using digital image capturing (20), has recently been developed. although it is emphasized that computerized image analysis has improved quantification, reproducibility and interobserver variability for the hr evaluation of breast carcinoma (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), the process…”

Section: Introductionmentioning

confidence: 99%

“…these attempts were performed using various systems or software, including cell analysis System's caS 100 (4), BiOcOm500 (5), caS 200 (6), image cytometry (7), adobe photoshop (8,9), computer-supported analysis (10), Spectracube™ (11), chroma Vision automated cellular imaging System (aciS) (12)(13)(14), WinrOOF (15), Qca (16) and ViSual c ++ (17). in particular, automated image analysis technology, including aQua (18), ariol (14,19) and matlab7 using digital image capturing (20), has recently been developed.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of evaluations of hormone receptors in breast carcinoma by image-analysis using three automated immunohistochemical stainings

Arihiro

Oda

Ogawa

et al. 2010

Experimental and Therapeutic Medicine

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Abstract. the aim of this study was to compare the results of immunohistochemistry (ihc) assays evaluated by human examiners with the results evaluated by computerized image analysis, and to compare the computerized image analysis results among three automated ihc assays, namely the BioGenex, dako and Ventana assays. all slides were semiquantitatively evaluated according to the allred score and J-score by human examiners. the images were analyzed using macScOpE version 2.6 for macintosh according to the h-score and the percentage of positive-stained nuclei per area of carcinoma cells (pp) irrespective of the intensity of the stained nuclei. the h-score for the estrogen receptor (Er) was significantly correlated with the Allred score (P<0.0001) and the PP for the ER was significantly correlated with the J-score (P<0.0001), suggesting that the image analysis used in the present study is a useful method for the evaluation of ER status. Several discrepancies were identified between the allred score and h-score and between the pp and J-score due to the positive-stained cytoplasm area of carcinoma cells and/ or the positive-stained nuclei area of non-carcinoma cells, including benign epithelial cells, lymphocytes and stromal cells. accordingly, advances in the algorithm of the digitized analyzing system is necessary.

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“…Esta dificuldade é ainda agravada pelo fato de que estes últimos também incluem um importante componente subjetivo como a determinação de campos e a definição do espectro colorimétrico a ser empregado. Mesmo considerando estas ressalvas, nossa experiência com a análise digital neste trabalho mostrou-se aparentemente confiável e coerente, confirmando os relatos favoráveis de outros estudos que utilizaram este recurso 18,19,20 . Assim sendo, concluímos não ser possível confirmar a partir deste estudo a hipótese de que os níveis de expressão imunoistoquímica da proteína VEGF nos carcinomas colorretais são maiores nos tumores com presença de metástase em linfonodos regionais, utilizando-se uma Matriz de Amostras Teciduais e análise de imagens assistida por computador.…”

Section: Grupounclassified

Análise da relação entre a expressão da proteína VEGF e o estadiamento do câncer colorretal

Mutschall

França

Ferreira

et al. 2009

Rev bras. colo-proctol.

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Interobserver agreement for estrogen receptor immunohistochemical analysis in breast cancer: a comparison of manual and computer-assisted scoring methods

Cited by 52 publications

References 15 publications

Diagnostic and Prognostic Importance of the Neutrophil Lymphocyte Ratio in Breast Cancer

Diagnostic and Prognostic Importance of the Neutrophil Lymphocyte Ratio in Breast Cancer

Comparison of evaluations of hormone receptors in breast carcinoma by image-analysis using three automated immunohistochemical stainings

Análise da relação entre a expressão da proteína VEGF e o estadiamento do câncer colorretal

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