International reference reagents to standardise blood group genotyping: evaluation of candidate preparations in an international collaborative study

Boyle, J.; Thorpe, Susan J.; Hawkins, J. Ross; C, Lockie; Fox, Bernard; Matejtschuk, Paul; Halls, Craig; Metcalfe, Paul; Rigsby, Peter; Armstrong-Fisher, S.S.; Varzi, Ali Mohammad; Urbaniak, S. J.; Daniels, Geoff

doi:10.1111/j.1423-0410.2012.01641.x

Cited by 17 publications

(14 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study revealed that genotyping using a microarray platform is a feasible blood group antigen genotyping tool that produces reliable data. The concordance rate with bidirectional sequencing was 100% and with serologic testing was 98·56%; these findings were in agreement with previous literature showing a 1% discrepancy rate, which could be attributed to the high rate of false‐positive results in serologic testing (Denomme & Oene, ; Kappler‐Gratias et al ., ; Bolye et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Blood group antigen and phenotype prevalence in the Korean population compared to other ethnic populations and its association with RBC alloantibody frequency

Jekarl

Yoo

Lee

et al. 2019

Transfusion Medicine

View full text Add to dashboard Cite

Objectives:This study aimed to analyse the allele frequency of blood group antigens in the Korean population and other ethnic populations and the association of blood group antigens with red blood cell (RBC) alloantibodies.Background: Blood group antigen genotyping can support patients undergoing frequent transfusions who have alloantibodies and antibodies against high-prevalence blood group antigens.Methods: Twenty-nine single nucleotide variations and 37 blood group antigens were tested. Samples requested for routine blood typing were collected from Jan to Apr 2016. Genotyping was performed on 145 Korean samples and was confirmed by bidirectional sequencing and serologic tests. The allele frequency data were compared with previous genotyping datasets (three datasets from Korea and one each from China, Europe, Asia, and the USA). Alloantibody frequencies and blood group antigens from the electronic medical record of 1772 cases were examined.Results: E antigen was higher in the Korean population compared to that of Asian and European populations. K, Kp a , Fy b and Do a allele frequencies were lower compared to other ethnic populations. RBC alloantibodies with frequencies (%) greater than 1% from the 1772 cases were as follows: anti-E, 36·7%, anti-C, 17·7%; anti-c 7·39%; anti-M, 5·9%; anti-e, 5·2%; anti-Jk a , 2·9%; and anti-Fy a , 1·1%. Blood group antigens and alloantibody frequencies revealed inverse trends that did not reach statistical significance. Conclusion:The allele frequency of blood group antigens assessed by high-throughput methods provided reliable and valuable information that could be used for maintaining donor pools and providing compatible blood for genotyped patients.

show abstract

Section: Discussionmentioning

confidence: 97%

Blood group antigen and phenotype prevalence in the Korean population compared to other ethnic populations and its association with RBC alloantibody frequency

Jekarl

Yoo

Lee

et al. 2019

Transfusion Medicine

View full text Add to dashboard Cite

show abstract

“…However, the preferred source of reference DNA is B‐lymphoblastoid cell lines, which should be established for all common alleles, while we may have to be content with homogeneous synthetic controls for many of the less common alleles. Use of reference reagents as quality control material, currently developed by the FDA (see Web resources) and ISBT, will be widely applied in the future. Similar to monoclonal antibodies, such reagents are easily available and, once established, pose no consent issues.…”

Section: Discussionmentioning

confidence: 99%

External quality assessment in molecular immunohematology: the INSTAND proficiency test program

et al. 2013

View full text Add to dashboard Cite

Background Genotyping for red blood cell (RBC), platelet and granulocyte antigens is a new tool for clinical pathology, transfusion medicine services and blood banks. Proficiency in laboratory tests can be established by external quality assessments (EQAs), which are required for clinical application in many health care systems. There are few EQAs for molecular immunohematology. Methods We analyzed the participation and pass rates in an EQA for RBC, platelet and granulocyte antigens. This EQA was distributed by INSTAND, a large non-profit provider of proficiency tests, twice per year since fall 2006 as EQA no. 235 Immunohematology A (molecular diagnostic). The coordinators defined at the outset which alleles are mandatory for detection. Results The number of participants steadily increased from 51 to 73 per proficiency by fall 2012. More than 60 institutions utilized this EQA at least once a year. Approximately 80% of them participated in RBC, 68% in platelet and 22% in granulocyte systems. With the exceptions of RHD (82%) and granulocytes (85%), pass rates exceeded 93%. While the pass rate increased for granulocyte and decreased for the ABO system, the pass rates for the other systems changed little over 6 ½ years. Conclusions The INSTAND proficiency test program was regularly used for EQA by many institutions, particularly in Central Europe. While the technical standards and pass rates in the participating laboratories were high, there has been little improvement in pass rates since 2006.

show abstract

“…The panel includes four DNA samples covering the most clinically important homozygous and heterozygous genotypes within RH, FY, KEL, JK, DO, and MNS blood group systems ( Table 4). The material was validated in an international collaborative study by PCR-ASP or PCR-SSP, PCR-RFLP, Multiplex SSP assays, real-time PCR, Immucor Beadchip array, Progenika BLOODchip array, Luminex array, and 5′ Nuclease assay [98].…”

Section: Molecular Testing: Standards For Rbc Antigensmentioning

confidence: 99%

Accuracy of Blood Group Typing in the Management and Prevention of Alloimmunization

Sippert¹,

Volkova²,

Rios³

2021

Human Blood Group Systems and Haemoglobinopathies

View full text Add to dashboard Cite

Blood transfusion is an effective therapeutic approach for several hematological conditions including sickle cell disease (SCD), thalassaemia, myelodysplastic syndrome (MDS), and autoimmune hemolytic anemia. It is also often indicated for transplantation and for patients receiving medical treatments for cancer. However, transfusion treatment can lead to the red blood cell (RBC) alloimmunization when an incompatible antigen is inadvertently present in the transfused blood. Alloantibodies can cause RBC destruction and many other complications defeating the purpose of the treatment. The risk of development of multiple alloantibodies increases with the frequency of transfusions in transfusion-dependent patients and can be mitigated by transfusing blood type negative for multiple antigens to prevent hemolysis. This chapter discusses the transfusion's risk of RBC alloimmunization as an adverse event; consequences of alloimmunization in patients' care; approaches to prevent and/or mitigate alloimmunization and enhance transfusion efficacy; application of RBC genotyping to supplement serology for preventing alloimmunization. The currently available techniques for RBC genotyping and the importance of reference reagents for determining the genotyping accuracy will also be discussed.Accuracy of Blood Group Typing in the Management and Prevention of Alloimmunization DOI: http://dx.doi.org/10.5772/intechopen.90095 Blood group system WHO IRR [98] CBER RR [89]Rh RHD positive (zygosity not determined)

show abstract

International reference reagents to standardise blood group genotyping: evaluation of candidate preparations in an international collaborative study

Abstract: The establishment of RBC1, RBC4, RBC5 and RBC12 as World Health Organization Reference Reagents will facilitate international standardisation of blood group genotyping and ensure that such tests are sufficiently sensitive and specific.

Cited by 17 publications

References 21 publications

Blood group antigen and phenotype prevalence in the Korean population compared to other ethnic populations and its association with RBC alloantibody frequency

Blood group antigen and phenotype prevalence in the Korean population compared to other ethnic populations and its association with RBC alloantibody frequency

External quality assessment in molecular immunohematology: the INSTAND proficiency test program

Accuracy of Blood Group Typing in the Management and Prevention of Alloimmunization

Contact Info

Product

Resources

About