Internalization of MWCNTs by microglia: Possible application in immunotherapy of brain tumors

Kateb, Babak; Handel, Michelle Van; Zhang, Leying; Bronikowski, Michael J.; Manohara, Harish; Badie, Behnam

doi:10.1016/j.neuroimage.2007.03.078

Cited by 145 publications

(83 citation statements)

References 36 publications

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“…This data validated the siCas 3 sequence used in this experiment and indicated that siCas delivered into cells is biologically active. of cells in vitro and in vivo (17,(33)(34)(35)(36). In this study, we directly administered stereotactically f -CNT (without siRNA) into the brain cortex to confirm their ability to be internalized by neuronal cells in vivo.…”

Section: Resultsmentioning

confidence: 95%

Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing

Al‐Jamal

Gherardini

Bardi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

212

122

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Stroke is the second cause of death worldwide with ischemic stroke accounting for 80% of all stroke insults. Caspase-3 activation contributes to brain tissue loss and downstream biochemical events that lead to programmed cell death after traumatic brain injury. Alleviation of symptoms following ischemic neuronal injury can be potentially achieved by either genetic disruption or pharmacological inhibition of caspases. Here, we studied whether silencing of Caspase-3 using carbon nanotube-mediated in vivo RNA interference (RNAi) could offer a therapeutic opportunity against stroke. Effective delivery of siRNA directly to the CNS has been shown to normalize phenotypes in animal models of several neurological diseases. It is shown here that peri-lesional stereotactic administration of a Caspase-3 siRNA (siCas 3) delivered by functionalized carbon nanotubes (f-CNT) reduced neurodegeneration and promoted functional preservation before and after focal ischemic damage of the rodent motor cortex using an endothelin-1 induced stroke model. These observations illustrate the opportunity offered by carbon nanotube-mediated siRNA delivery and gene silencing of neuronal tissue applicable to a variety of different neuropathological conditions where intervention at well localized brain foci may offer therapeutic and functional benefits.nanomedicine | neurodegenerative | neuroprotection | neurosciences | gene therapy

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Section: Resultsmentioning

confidence: 95%

Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing

Al‐Jamal

Gherardini

Bardi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

212

122

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show abstract

“…It is possible that small amounts of MWCNTs were detached from the scaffold and internalized by NSCs. Some studies have demonstrated that internalization of MWCNTs by neural cells can induce neural differentiation including activation of glial cells [47][48][49][50]. Bardi et al examined internalization of functionalized MWCNTs.…”

Section: Electrical Pulse Stimulation Promotes the Neuronal And Oligomentioning

confidence: 99%

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

et al. 2018

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Objective. Nanomaterials, such as carbon nanotubes (CNTs), have been introduced to modify the surface properties of scaffolds, thus enhancing the interaction between the neural cells and biomaterials. In addition to superior electrical conductivity, CNTs can provide nanoscale structures similar to those present in the natural neural environment. The primary objective of this study is to investigate the proliferative capability and differential potential of neural stem cells (NSCs) seeded on a CNT incorporated scaffold. Approach. Amine functionalized multi-walled carbon nanotubes (MWCNTs) were incorporated with a PEGDA polymer to provide enhanced electrical properties as well as nanofeatures on the surface of the scaffold. A stereolithography 3D printer was employed to fabricate a well-dispersed MWCNT-hydrogel composite neural scaffold with a tunable porous structure. 3D printing allows easy fabrication of complex 3D scaffolds with extremely intricate microarchitectures and controlled porosity. Main results. Our results showed that MWCNT-incorporated scaffolds promoted neural stem cell proliferation and early neuronal differentiation when compared to those scaffolds without the MWCNTs. Furthermore, biphasic pulse stimulation with 500 µA current promoted neuronal maturity quantified through protein expression analysis by quantitative polymerase chain reaction. Significance. Results of this study demonstrated that an electroconductive MWCNT scaffold, coupled with electrical stimulation, may have a synergistic effect on promoting neurite outgrowth for therapeutic application in nerve regeneration.

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“…153 MWCNTs (Multiwall carbon nanotube) showed a high level of internalization of macromolecules in microglial cells and their molecular modulation helped in immunotherapy of cancer. 154 Folic acid (as targeting agent) and methotrexate conjugated PAMAM dendritic polymers bind to tumor cell which overexpressed for folate receptor in cancer treatment. 155 Boron-enriched nanocomposites of copolymerized acetal-poly(ethylene glycol)-block-poly(lactide)-methacrylate with 4-vinylbenzyl substituted closocarborane demonstrated high incorporation and hemocompatibility.…”

Section: Tumormentioning

confidence: 99%

Nanomedicine in Central Nervous System (CNS) Disorders: A Present and Future Prospective

Soni¹,

Ruhela²,

Medhi³

2016

Adv Pharm Bull

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Internalization of MWCNTs by microglia: Possible application in immunotherapy of brain tumors

Cited by 145 publications

References 36 publications

Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing

Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

Nanomedicine in Central Nervous System (CNS) Disorders: A Present and Future Prospective

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