Internal Yellowing, a Bacterial Disease of Papaya Fruits Caused by Enterobacter cloacae

Nishijima, K. A.; Couey, H. Melvin; Alvarez, Anne M.

doi:10.1094/pd-71-1029

Cited by 44 publications

(29 citation statements)

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“…2C) on TTC medium (1 gl −1 casamino acid, 10 gl −1 peptone, 5 gl −1 glucose, 17 gl −1 agar, 0.05 gl −1 triphenyltetrazolium chloride; pH 7.2). Pure cultures of each strain were maintained in frozen aliquots in 20% glycerol at −80°C and in 5 ml sterile distilled water (SDW) in screw-cap tubes (Nishijima et al 1987). Two reference strains E. cancerogenus LMG2693 and E. cloacae subsp.…”

Section: Methodsmentioning

confidence: 99%

“…Two of these pathogens cause diseases in woody plants: E. cancerogenus causes poplar canker (Dickey and Zumoff 1988) and E. nimipressuralis causes elm wetwood disease (Carter 1964;Murdoch and Campana 1983). Variants of E. cloacae have been associated with internal yellowing disease of papaya (Nishijima et al 1987), internal decay of onion (Bishop and Davis 1990) and rhizome rot of ginger (Nishijima et al 2004). Recently it was reported that Enterobacter sakazakii (renamed Cronobacter sakazakii) (Iversen et al 2007) induces an atypical internal yellowing of papaya fruit in Hawaii (Keith et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of the Enterobacter complex causing mulberry (Morus alba) wilt disease in China

et al. 2009

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Mulberry wilt disease (MWD) was recently identified in Hangzhou, Zhejiang province, China. Typical symptoms of the disease are browning of vascular tissues, leaf wilt, defoliation, and tree decline. Unlike the symptoms of bacterial wilt disease caused by Ralstonia solanacearum, symptoms of MWD generally started from the bottom of the plants and moved upward. In inoculation experiments, four selected MWD strains caused mulberry shoot leaf wilt, discoloration, and defoliation. They also induced whole plant leaf wilt, defoliation and dark brown discoloration of vascular tissue. Based on Biolog metabolic profiles, fatty acid methyl ester analysis (FAME) and sequence analysis of the partial 16S rDNA and rpoB genes four MWD strains were identified as members of the genus Enterobacter. The 16S rDNA and rpoB gene sequences revealed a close relationship among two isolates, R2-2 and R6-2, and the E. asburiae type strain JCM6051. The isolates showed >98% similarity to E. asburiae JCM6051 in their rpoB gene. These results indicated that isolates R2-2 and R6-2 belonged to E. asburiae. No similarity in 16S rDNA sequences above 97% was found between either of the remaining isolates, R11-2 or R18-2, and any recognized Enterobacter species, suggesting that the two isolates may represent novel Enterobacter species. rpoB gene similarity values between the isolates and Enterobacter spp. type strains were <98%, providing further evidence that the two isolates may represent a novel species within the Enterobacter. The causal agent for MWD was previously reported to be E. cloacae, however, this study found that other Enterobacter spp. (E. asburiae and Enterobacter sp.) also cause MWD.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and characterization of the Enterobacter complex causing mulberry (Morus alba) wilt disease in China

et al. 2009

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“…Despite wide variation of the strains, there is no evidence of physiological or pathological specialization attributable to strains of E. cloacae isolated from different sources. In fact, some strains of E. cloacae are opportunistic pathogens of plants (Bishop 1990;Nishijima and Couey 1987;Takahashi et al 1997), which called our attention to a potential risk: that indiscreet use of nonspecialized strains of E. cloacae may cause unexpected persistence in the natural environment and, in some cases, opportunistic diseases in untargeted plants and animals. To minimize such ecological risks, it is a primary task to clarify the movement or behavior of E. cloacae released into the environment and to differentiate the introduced bacteria from a background of genetically similar and dissimilar indigenous bacteria.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of chitosanase activity in chitosanase gene-transformed strains of Enterobacter cloacae by lytic infection of specific bacteriophages

Takikawa

Ishii

Matsuda

et al. 2003

Journal of General Plant Pathology

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In combination with lytic infection by virulent phages, a simple method for monitoring transgenic strains of Enterobacter cloacae was developed in this study. First, 15 strains of E. cloacae were used as indicator bacteria to isolate virulent phages with different host ranges. Of the phages isolated, five isolates were used to construct a set of virulent phages corresponding to all strains of E. cloacae. Using this phage set, a rhizosphere strain (KRM-055E) of E. cloacae was effectively screened from field soil. KRM-055E was transformed with a prokaryotic chitosanase gene csnSM1 and infected with the phage EcP-03, which can lyse the strain most effectively. The lysis of KRM-055E/csn occurred 2 h after inoculation, and the chitosanase activity was simply detected by dropping the lysate onto an agar plate containing glycol chitosan. The positive signal for chitosanase activity was detected in the 2-h lysates, and the signal intensity reached a maximum in the 5-h lysate. The present assay was simple, rapid, inexpensive, easy to perform, and applicable to another strains.

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“…Enterobacter nimipressuralis causes wet wood disease of elm (Carter 1964;Murdoch and Campana 1983), E. cancerogenus causes poplar canker (Dickey and Zumoff 1988), and E. cloacae causes internal yellowing disease of papaya (Nishijima et al 1987), internal decay of onion (Bishop and Davis 1990) and rhizome rot of ginger (Nishijima et al 2004). E. cloacae, E. asburiae and Enterobacter sp.…”

mentioning

confidence: 99%

First report on Enterobacter sp. causing soft rot of Amorphophallus konjac in China

Ding

Diao

et al. 2011

J Gen Plant Pathol

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Internal Yellowing, a Bacterial Disease of Papaya Fruits Caused by Enterobacter cloacae

Cited by 44 publications

References 0 publications

Identification and characterization of the Enterobacter complex causing mulberry (Morus alba) wilt disease in China

Identification and characterization of the Enterobacter complex causing mulberry (Morus alba) wilt disease in China

Rapid detection of chitosanase activity in chitosanase gene-transformed strains of Enterobacter cloacae by lytic infection of specific bacteriophages

First report on Enterobacter sp. causing soft rot of Amorphophallus konjac in China

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