First report on Enterobacter sp. causing soft rot of Amorphophallus konjac in China

Wu, Jinping; Ding, Zhong‐Tao; Diao, Youxiang; Hu, Zhongli

doi:10.1007/s10327-011-0330-1

Cited by 11 publications

(4 citation statements)

References 9 publications

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“…Both bacterial species are in the soft rot Pectobacteriaceae (SRP) family. Recently, Enterobacter asburiae was found to cause mulberry wilt disease and soft rot on Amorphophallus konjac in China (Wang et al, 2010;Wu et al, 2011). E. asburiae is a gram-negative, facultative anaerobic, oxidase negative, non-motile, and non-pigmented rod-shaped species isolated from soil, water, and food products and previously known as an epiphytic bacteria (Lau et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Genome Structure of Virulent Phage EspM4VN to Control Enterobacter sp. M4 Isolated From Plant Soft Rot

et al. 2020

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Enterobacter sp. M4 and other bacterial strains were isolated from plant soft rot disease. Virulent phages such as EspM4VN isolated from soil are trending biological controls for plant disease. This phage has an icosahedral head (100 nm in diameter), a neck, and a contractile sheath (100 nm long and 18 nm wide). It belongs to the Ackermannviridae family and resembles Shigella phage Ag3 and Dickeya phages JA15 and XF4. We report herein that EspM4VN was stable from 10 • C to 50 • C and pH 4 to 10 but deactivated at 70 • C and pH 3 and 12. This phage formed clear plaques only on Enterobacter sp. M4 among tested bacterial strains. A one-step growth curve showed that the latent phase was 20 min, rise period was 10 min, and an average of 122 phage particles were released from each absorbed cell. We found the phage's genome size was 160,766 bp and that it annotated 219 open reading frames. The genome organization of EspM4VN has high similarity with the Salmonella phage SKML-39; Dickeya phages Coodle, PP35, JA15, and Limestone; and Shigella phage Ag3. The phage EspM4VN has five tRNA species, four tail-spike proteins, and a thymidylate synthase. Phylogenetic analysis based on structural proteins and enzymes indicated that EspM4VN was identified as a member of the genus Agtrevirus, subfamily Aglimvirinae, family Ackermannviridae.

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Section: Introductionmentioning

confidence: 99%

Characterization and Genome Structure of Virulent Phage EspM4VN to Control Enterobacter sp. M4 Isolated From Plant Soft Rot

et al. 2020

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“…Enterobacter has been reported to cause soft rot of papaya, amorphophallus konjac and potato, [37][38][39] Klebsiella has been reported to cause soft rot of carrot and pepper, 40,41 Pseudomonas has been reported to cause soft rot of pepper, carrot and melon [42][43][44] and Bacillus has also been reported to cause soft rot of pepper and ginger rhizome. 1,45,46 Therefore, in order to reduce losses of postharvest peppers, Pectobacterium is not the only control target, but the most important one.…”

Section: Discussionmentioning

confidence: 99%

Soft rot of postharvest pepper: bacterial pathogen, pathogenicity and its biological control using Lactobacillus farciminis LJLAB1

Li,

et al. 2023

J Sci Food Agric

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BackgroundSoft rot is the most important bacterial disease of postharvest pepper during storage and transportation. The main objectives of this study were to investigate the bacterial pathogen species causing pepper soft rot and seek for an antagonistic bacterium to control this disease.ResultsPathogens Pectobacterium carotovorum, Enterobacter sp., Klebsiella sp., Pseudomonas sp. and Bacillus sp. were verified to be the causes of soft rot which were isolated from rotten peppers. Among them, P. carotovorum had the highest prevalence, including P. carotovorum subsp. carotovorum (Pcc) and P. carotovorum subsp. brasilisesis (Pcb). The result of pathogenicity analysis showed that Pcb Jm2 had strong pathogenicity at 25 °C even at a cell concentration of 103 CFU mL−1. Its pathogenicity decreased at 4 °C. Multiple pathogenic factors were identified in the draft genome of Pcb Jm2, including cellulase, pectinase, pectin methylesterase, pectinesterase, pectin lyase, polygalacturonase and so forth. Further, the disease control ability of Lactobacillus farciminis LJLAB1 was investigated. The cell‐free supernatant (CFS) and crude bacteriocin of L. farciminis LJLAB1 had good antibacterial activities to Pcb Jm2 in vitro, but CFS exhibited a better disease control effect in vivo. CFS treatment prevented the damage of pepper epidermal structure caused by Pcb Jm2, and 99.26% of pathogen cells on pepper were killed by it. Moreover, CFS treatment delayed firmness decrease, soluble solid content loss, weight loss, yellowing and malonaldehyde accumulation of pepper during storage after pathogen infection.ConclusionL. farciminis LJLAB1 can be an effective biological control agent to control pepper soft rot caused by Pcb. © 2023 Society of Chemical Industry.

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“…However, some of them have been reported as phytopathogens, such as Enterobacter asburiae isolated from konjac ( Amorphophallus konjac ) in China and Enterobacter cloacae isolated from chili pepper ( Capsicum annuum L.) and dragon fruit ( Hylocereus spp.) ( 4 – 7 ). Identification of the order Enterobacteriales has been difficult with the 16S rRNA gene approach and other single-gene and limited multigene approaches ( 2 ).…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequence of Enterobacter kobei M4-VN, Isolated from Potatoes with Soft Rot Disease

Thành

Fujino

Hiromasa

et al. 2020

Microbiol Resour Announc

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First report on Enterobacter sp. causing soft rot of Amorphophallus konjac in China

Cited by 11 publications

References 9 publications

Characterization and Genome Structure of Virulent Phage EspM4VN to Control Enterobacter sp. M4 Isolated From Plant Soft Rot

Characterization and Genome Structure of Virulent Phage EspM4VN to Control Enterobacter sp. M4 Isolated From Plant Soft Rot

Soft rot of postharvest pepper: bacterial pathogen, pathogenicity and its biological control using Lactobacillus farciminis LJLAB1

Draft Genome Sequence of Enterobacter kobei M4-VN, Isolated from Potatoes with Soft Rot Disease

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