Draft Genome Sequence of Enterobacter kobei M4-VN, Isolated from Potatoes with Soft Rot Disease

Thành, Nguyễn Công; Fujino, Yasuhiro; Hiromasa, Yasuaki; Doi, Kunio

doi:10.1128/mra.00908-20

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…To further investigate the Pb-resistant genes and removal mechanism and metabolic pathways, whole-genome sequencing (WGS) was performed for E. kobei FACU6. The genome was larger than previously reported E. kobei M4-VN that was previously disclosed (Thanh et al, 2020). E. kobei FACU6 contained a high tRNA gene content.…”

Section: Discussionmentioning

confidence: 63%

Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium

El-Beltagi,

Halema,

Almutairi

et al. 2024

Front. Bioeng. Biotechnol.

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Lead pollution of the environment poses a major global threat to the ecosystem. Bacterial bioremediation offers a promising alternative to traditional methods for removing these pollutants, that are often hindered by various limitations. Our research focused on isolating lead-resistant bacteria from industrial wastewater generated by heavily lead-containing industries. Eight lead-resistant strains were successfully isolated, and subsequently identified through molecular analysis. Among these, Enterobacter kobei FACU6 emerged as a particularly promising candidate, demonstrating an efficient lead removal rate of 83.4% and a remarkable lead absorption capacity of 571.9 mg/g dry weight. Furthermore, E. kobei FACU6 displayed a remarkable a maximum tolerance concentration (MTC) for lead reaching 3,000 mg/L. To further investigate the morphological changes in E. kobei FACU6 in response to lead exposure, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed. These analyses revealed significant lead adsorption and intracellular accumulation in treated bacteria in contrast to the control bacterium. Whole-genome sequencing was performed to gain deeper insights into E. kobei’s lead resistance mechanisms. Structural annotation revealed a genome size of 4,856,454 bp, with a G + C content of 55.06%. The genome encodes 4,655 coding sequences (CDS), 75 tRNA genes, and 4 rRNA genes. Notably, genes associated with heavy metal resistance and their corresponding regulatory elements were identified within the genome. Furthermore, the expression levels of four specific heavy metal resistance genes were evaluated. Our findings revealed a statistically significant upregulation in gene expression under specific environmental conditions, including pH 7, temperature of 30°C, and high concentrations of heavy metals. The outstanding potential of E. kobei FACU6 as a source of diverse genes related to heavy metal resistance and plant growth promotion makes it a valuable candidate for developing safe and effective strategies for heavy metal disposal.

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Section: Discussionmentioning

confidence: 63%

Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium

El-Beltagi,

Halema,

Almutairi

et al. 2024

Front. Bioeng. Biotechnol.

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“…The bacteria E. kobei, E. cloacae, B. gaviniae and B. warmboldiae were found in fruit and cream cakes, and K. oxytoca in chocolate and cream cakes (Figure 1). Thanh et al (2020) identified E. kobei bacteria in potatoes, and Nyenje et al (2012) E. cloacae in ready-to-eat food (RTE food) from South Africa. B. gaviniae has been detected in snails and drinking water (Muller et al, 1996) and raw salmon (Mace et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Identification of bacteria species among Enterobacteriaceae found in confectionery cakes

Petrić,

Hengl,

Kovaček

et al. 2023

Hrvat. čas. prehrambenu tehnol. biotehnol. nutr. (Online)

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The goal of this research was to identify which Enterobacteriaceae species are present in cakes from confectionery establishments and whether these are species that can be considered pathogenic for humans and what their harmful impact on human health could be. The sampling of confectionery cakes was carried out in the area of the City of Zagreb, and the samples were analysed for the presence of Enterobacteriaceae in accordance with the HRN ISO 21528-2:2017 standard. In cases where the samples contained the number of Enterobacteriaceae greater than 102 CFU/g, the MALDI-TOF method was used to identify individual species of enterobacteria. The results of the analyses determined the presence of 10 Enterobacteriaceae species: Enterobacter kobei, Enterobacter cloacae, Pantoea agglomerans, Serratia liquefaciens, Enterobacter asburiae, Klebsiella oxytoca, Buttiauxella gaviniae, Buttiauxella warmboldiae, Raoultella and Cedecea neteri. All species of Enterobacteriaceae determined in this research, according to the literature, were previously isolated from food or water, and all species except Buttiauxella warmboldiae were isolated from humans and the environment. However, for none of the species of Enterobacteriaceae determined in this research a literature reference to support food as a vehicle in case of human illness was found.

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Draft Genome Sequence of Enterobacter kobei M4-VN, Isolated from Potatoes with Soft Rot Disease

Abstract: Enterobacter kobei M4-VN, isolated from potatoes with soft rot disease in Vietnam, contains a total of 4,754,309 bp with 4,424 predicted coding sequences and a G+C content of 55.1%.

Cited by 2 publications

References 22 publications

Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium

Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium

Identification of bacteria species among Enterobacteriaceae found in confectionery cakes

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