Internal Validation of Rapid and Performant Method for Carotenoids Determination in Tomato Waste Powder by HPLC

Abstract

An analytical method was developed and validated for separation, detection and quantification of carotenoids (all-trans lutein, β-carotene and all-trans lycopene) in tomato waste powder by highperformance liquid chromatography (HPLC-DAD). Extraction of carotenoids was achieved in acetone under nitrogen atmosphere and magnetic stirring. Carotenoids were separated on a reverse-phase C30, 3 μm column (250 ×4.6 mm) coupled to a 20 × 4.6 mm C30 guard column using mobile phases consisting of (A) methanol/ water (98:2, v/v), (B) methanol/water (95:5, v/v) and (C) methyl tert-butyl ether.The method has a good sensitivity (LOD = 0.161 -0.333 μg/mL and LOQ = 0.484 -1.000 μg/mL) and a good precision (RDS (r) = 0.67 -1.15% for injection repeatability; RSD (r) = 1.02 -2.14% for analysis repeatability intra-day; RSD (r) = 1.23 -2.43% for intermediate precision; RSD (R) = 1.57 -3.07 % for intra-laboratory reproducibility. The method was applied byanalyzing 8 tomato waste powders, obtained through tomatoes processing as juice. Their carotenoids content varied in the following ranges: 1. 474 -2.452 mg/100g for all-trans lutein; 9.645 -11.587 mg/100g for β-carotene; 60.150 -64.855 mg/100g for all-trans lycopene.

Antioxidant Capacity, Sugar Content, and Tandem HPLC–DAD–ESI/MS Profiling of Phenolic Compounds from Aronia melanocarpa Fruits and Leaves (Nero and Viking Cultivars)

“…The highest total phenolic content was also observed in the samples from Bursa. Similar findings as of the present study were reported in some other previous studies as 1455.2 mg GAE/ 100 g and 1388 mg GAE/100 g. 6,27 However, Ochmian et al 28 determined higher total phenolic contents of chokeberry fruits from four cultivars as follows: Nero: 1950 and Viking: 1845 mg GAE/100 g compared to the current study. On the other hand, Jakobek et al 11 reported phenolic contents in chokeberry fruits of four cultivars (Nero, Viking, Galicianka, and wild type) with the Viking cultivar having the highest during two consecutive years (10804.1 mg GAE/kg in 2010 and 12055.7 mg GAE/kg in 2011).…”

“…A total of 19 phenolic compounds were detected in all chokeberry leaf samples. The phenolic groups found in the leaves were flavonols (11), phenolic acid and derivatives (6), and flavanols (2). Unlike the chokeberry fruits, anthocyanins were not detected in chokeberry leaves.…”

“…4−7 Although aronia fruit can be consumed fresh, it is usually processed into alternative products such as dried fruit, fruit puree, fruit juice, jam, tea, liquor, and wine due to its sour and bitter taste. 2,6,7 It is also used as an ingredient in functional foods and dietary supplements due to its high anthocyanin content and as a natural colorant in the food and pharmaceutical industry. 8 Aronia fruit has a unique bioactive composition along with phenolic groups such as anthocyanins, procyanidins, phenolic acids, flavonols, dietary fiber (∼5%), reducing sugar (16−18%), organic acids (mainly malic and citric acids), vitamins (B and C groups), and minerals.…”

“…18,19 The total phenolic content of fruits and leaves may vary depending on the variety, location, climatic conditions, ripening, and harvest time. 6,20 Aronia plant grown in different regions is exposed to differing climatic conditions (temperature, precipitation, number of sunny days, etc. ), resulting in significant differences in the amount of phenolic compounds.…”

A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry

“…The average recovery value obtained is of 98.53 (±0.08)% (n=5) for lysine 0.1, 0.2, 0.4, 06, 0.8 and 0.9 mmol L -1 , which is within the ICH, USEPA and Commission Decision 2002/657/EC [53] quality control criteria [30][31][32]48,54,55], where the limits for recovery values are in the range of 80 and 120 % (Table 4). For Lysine-KCl, the average recovery value is of 102.95 (±0.2) % (n=5) for the same above mentioned concentrations of lysine.…”