Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey

Abstract

Twenty-three populations belonging to 10 wild Primula L. (Primulaceae) taxa of Turkey, some of which are morphologically quite similar, were investigated based on nrDNA ITS regions. The plant materials were collected from different geographical areas of Anatolia in vegetation periods of 2005 and 2006. Total genomic DNAs were isolated from the healthy leaves of each population. The entire ITS regions of the populations were amplified by universal primers with the aid of a polymerase chain reaction (PCR), and then the PCR products were sequenced. Neighbour-Joining (NJ) and Maximum Parsimony (MP) trees were constructed in order to identify the relationships among Primula taxa. According to ITS data, 724 characters were determined among the aligned sequences of the populations and the divergence values were found to be between 0.0% and 20.9%. ITS sequences from 23 specimens provided a number of variable and sufficient characters to explore the relationships. As a result, it was determined that the dendrograms obtained by NJ and MP analysis are concordant with the traditional taxonomical order at subgeneric level.

Comprehensive assessment of genetic variation in native heterostylous primrose genotypes of Türkiye

“…Table 6. Table 6 (CA)8R -400 0,084 0,114 0,125 -0,084 -0,023 -0,143 0,115 0,138 -0,135 0,214 -0,257 -0,103 0,35 -0,037 BDB(CA)7C-400 0,127 -0,038 -0,004 -0,216 0,029 -0,218 -0,017 -0,001 -0,025 0,01 0,011 -0,098 -0,075 -0,012 BDB(CA)7C-500 0,057 0,165 0,205 0,095 0,18 -0,247 -0,331 -0,108 -0,163 0,123 0,264 0,123 0,159 0,04 BDB(CA)7C-800 0,169 0,071 0,107 -0,15 0,125 0,004 -0,109 -0,241 0,165 0,157 0,267 0,331 -0,119 0,009 (TC)8TG -450 0,181 -0,223 -0,268 -0,181 -0,02 0,293 0,251 -0,097 0,186 -,487** -0,034 -0,005 -0,352 -0,13 12 (TC)8TG -800 -0,193 0,09 0,136 0,344 0,028 -0,3 -0,193 -0,194 0,019 -0,036 0,08 -0,062 -0,027 -0,134 (TC)8TG-1000 -0,209 0,121 0,135 0,353 0,032 -0,343 -0,221 -0,054 -0,036 0,025 0,085 -0,127 0,042 -0,017 In a study conducted in the same region where Primula genotypes were previously sampled, Gültepe et al (2010) examined 23 populations belonging to 10 native Primula L. taxa. They analyzed the nrDN ITS regions and performed base sequence analysis to determine the phylogenetic relationship between Primula taxa.…”

“…There are many studies on the genus Primula, and morphological (Smith and Fletcher, 1948;Zhang and Kadereit, 2005), cytological (Bruum, 1932), biochemical (Yayli, 2001;Morozowska, 2004;Fico et al, 2007. ), and molecular (Cservenka et al, 2002;Zhang and Kadereit, 2004;Zhang and Kadereit, 2005;Manfield et al, 2005;Gültepe et al, 2010) studies have been carried out.…”

Tragopogon davutii‐turanii: a new species of Tragopogon from Anatolia

“…Genomic DNA was extracted from herbarium materials following the modified extraction procedure of Doyle and Doyle (1987). Amplification and sequencing of nrDNA ITS regions were carried out according to Gültepe et al (2010). Phylogenetic analyses were performed on an ITS dataset consisting of 115 accessions, four of which were newly generated sequences (individuals belonging to the new species), and a sequence belonging to T. vvedenskyi obtained from GenBank (KF050362); 110 sequences, thirteen of which were used as outgroups, were obtained from Gültepe et al (2021).…”

Contribution to the taxonomy of little known Tragopogon species endemic to Turkey

“…The ITS region and the trn L intron with trn L‐F region were amplified with the universal primers ITS4/ITS5 (White et al 1990) and trnC/trnF (Taberlet et al 1991), respectively. For ITS‐PCR conditions, Gültepe et al (2010) were followed. The amplification process of the trn L intron with trn L‐F region was performed in 50 μl of PCR reaction volume containing 10 mM of Taq polymerase reaction buffer (10 μl), 2 mM of magnesium chloride (2.5 μl), 200 mM of dNTP mix (20 μl), 1 µl trnC and 1µl for trnF primers (1 µM each), 2 units of Taq DNA polymerase, 2–6 ng (1 μl of 2–6 ng μl −1 ) of total template DNA and 14 μl of ddH 2 O.…”