Internal hydrocephalus, optic nerve aplasia, and microphthalmia in CPB-WE (Wezob) and Cpb: WU (Wistar) rats

Eden, C.G. van; Mullink, J. W. M. A.

doi:10.1258/002367786780865548

Cited by 9 publications

(3 citation statements)

References 12 publications

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“…Later studies also reported high incidence rates (20%–40%) of hydrocephalus in many other albino experimental rats, including hydrocephalic Texas (H-Tx) (2), Csk: Wistar-Imamichi (WIC-Hyd) (3), Wistar-Lewis (LEW/Jms) (4, 5), Sprague-Dawley (6), Wezob (CPB-WE) and Wistar (Cpb: WU) (7) rats.…”

Section: Introductionmentioning

confidence: 95%

Imaging of Spontaneous Ventriculomegaly and Vascular Malformations in Wistar Rats: Implications for Preclinical Research

Turtzo

Williams

et al. 2014

J Neuropathol Exp Neurol

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Wistar rats are widely used in biomedical research and commonly serve as a model organism in neuroscience studies. In most cases when noninvasive imaging is not utilized, studies assume a consistent baseline condition in rats that lack visible differences. While performing a series of traumatic brain injury studies, we discovered mild spontaneous ventriculomegaly in 70/162 (43.2%) of Wistar rats that had been obtained from 2 different vendors. Advanced magnetic resonance (MR) imaging techniques, including MR angiography and diffusion tensor imaging, were utilized to evaluate the rats. Multiple neuropathologic abnormalities, including presumed arteriovenous malformations, aneurysms, cysts, white matter lesion and astrogliosis were found in association with ventriculomegaly. Postmortem micro-CT and immunohistochemical staining confirmed the presence of aneurysms and arteriovenous malformations. Diffusion tensor imaging significant decreases in fractional anisotropy and increases in mean diffusivity, axial diffusivity, and radial diffusivity in multiple white matter tracts (p < 0.05). These results could impact the interpretation, e.g. of a pseudo-increase of axon integrity and a pseudo-decrease of myelin integrity, based on characteristics intrinsic to rats with ventriculomegaly. We suggest the use of baseline imaging to prevent the inadvertent introduction of a high degree of variability in preclinical studies of neurological disease or injury in the Wistar rats.

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Section: Introductionmentioning

confidence: 95%

Imaging of Spontaneous Ventriculomegaly and Vascular Malformations in Wistar Rats: Implications for Preclinical Research

Turtzo

Williams

et al. 2014

J Neuropathol Exp Neurol

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“…In Wistar rats of the CPB-WE strain, a high incidence of hydrocephalus, accompanied by optic nerve aplasia and microphthalmia has been reported, but no histologic findings have been described in the ocular lesions. 8 We encountered two cases of unilateral optic nerve aplasia among 200 male and 200 female Slc: Wistar rats that were 6 weeks of age. Both cases were females.…”

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Unilateral Optic Nerve Aplasia in Two Young Slc: Wistar Rats

Shibuya¹,

Tajima²,

Yamate³

et al. 1989

Vet Pathol

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Congenital aplasia and hypoplasia of the optic nerves are rarely seen in human beings4 and animak6 Similar conditions occur infrequently in but no detailed description of the condition has been given, except for the Bmn-wys strain of rat. Bmn-wys rats are animal models of inherited retinal degeneration; aplasia or hypoplasia of the optic nerve occurs in association with microphthalmosI0 in these rats. In Wistar rats of the CPB-WE strain, a high incidence of hydrocephalus, accompanied by optic nerve aplasia and microphthalmia has been reported, but no histologic findings have been described in the ocular lesions.8We encountered two cases of unilateral optic nerve aplasia among 200 male and 200 female Slc: Wistar rats that were 6 weeks of age. Both cases were females. The animals were obtained from Shizuoka Laboratory Animal Center (Shizuoka, Japan) and maintained under barrier conditions in a room controlled at 23 C k 2 C, 50% f 20% relative humidity, and a 12-hour lightdark cycle. Standard commercial laboratory diet for rats (CRF-1, Charles River Japan Inc., Kanagawa, Japan) and chlorinated tap water were available ad libitum.The affected animals showed no noticeable clinical signs. Ophthalmic examination revealed a fixed pupil in the left eye of one case (Case l), and fundus examination by ophthalmoscopy disclosed a grayish brown fundus, absence of the optic disk and of the central retinal blood vessels in the left eyes in both cases. No abnormalities were observed in the right eyes of either animal.The animals were subjected to a complete necropsy. In both animals, the left optic nerve was completely missing from the posterior pole of the eyeball to the optic chiasma. The right optic nerve appeared normal in size; the right optic tract was rudimentary and barely visible (Fig. 1). The left eyeball with no optic nerve was externally normal and similar in size to the normal right eyeball. No abnormalities other than those described above were observed. The eyeballs, optic nerves, and brains were fixed in 10% neutral buffered formalin. The tissues were embedded in paraffin, sectioned, and stained with hematoxylin and eosin (HE), luxol fast blue and HE, as well as by the Bodian and Holzer methods. Thickness of the retinas from the eyes with no optic nerve or with the normal optic nerve from both cases was measured at the central and peripheral regions with an eyepiece micrometer. The retinas from five, healthy, age-matched Slc : Wistar rats were also measured in an identical manner and served as controls.Similar histologic abnormalities were observed in both animals, so they will be described together. The lesions observed were aplasia of the optic disk, retinal hypoplasia, rosette formations in the retina and hypoplasia of the ciliary body (Fig. 2). Case 1 revealed subretinal hemorrhage and nodular hyperplasia of the choroid, in which calcified foci were surrounded by thin connective tissue (Fig. 2). The inner portion of the retina, consisting of the inner limiting membrane and the layers of optic nerve fibers, ga...

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“…Sasaki reported a congenital hydrocephalic rat originating in the LEW/ Jms line [8], and Higashi also reported LEW/Jms hydrocephalus [3]. Van Eden observed a high incidence of hydrocephalus in CPB-WE and Cpb ; WU rats [9].…”

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Inherited Hydrocephalus in Csk: Wistar-Imamichi Rats; Hyd Strain: a New Disease Model for Hydrocephalus

Koto

Miwa

Shimizu

et al. 1987

Experimental Animals

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Internal hydrocephalus, optic nerve aplasia, and microphthalmia in CPB-WE (Wezob) and Cpb: WU (Wistar) rats

Cited by 9 publications

References 12 publications

Imaging of Spontaneous Ventriculomegaly and Vascular Malformations in Wistar Rats: Implications for Preclinical Research

Imaging of Spontaneous Ventriculomegaly and Vascular Malformations in Wistar Rats: Implications for Preclinical Research

Unilateral Optic Nerve Aplasia in Two Young Slc: Wistar Rats

Inherited Hydrocephalus in Csk: Wistar-Imamichi Rats; Hyd Strain: a New Disease Model for Hydrocephalus

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