Internal extractive electrospray ionization mass spectrometry for investigating the phospholipid dysregulation induced by perfluorooctanoic acid in Nile tilapia

Abstract

Direct profiling endogenous biomolecules in tissue sample is considered as a promising approach to investigate metabolic-related toxicity on organisms induced by emerging pollutants. Herein, we report the application of internal...

Liver Metabolic Dysregulation Induced by Polypropylene Nano- and Microplastics in Nile Tilapia Using Internal Extractive Electrospray Ionization Mass Spectrometry

“…15−17 To some extent, it limits the development of environmental metabonomics and environmental toxicology. 18 Therefore, the development of new mass spectrometry techniques for rapid, efficient, accurate, real-time, in situ, and online analysis of metabolites in complex biological samples has become a new requirement for the construction of environmental metabolomics and environmental toxicology.…”

“…Mass spectrometry (MS) has become an important platform, analyzing the effects of exogenous pollutants on biological metabolism at the molecular level. In nontargeted metabonomics analysis, traditional mass spectrometry analysis methods such as liquid chromatography-MS (LC-MS) and gas chromatography-MS (GC-MS) typically require labor-intensive pretreatment procedures like grinding, extraction, and separation. , As a result, those conventional means are comparatively time-consuming, unable to meet the actual needs of high-throughput analysis for numerous samples, and inevitably lead to the loss of some active components in tissue samples. − To some extent, it limits the development of environmental metabonomics and environmental toxicology . Therefore, the development of new mass spectrometry techniques for rapid, efficient, accurate, real-time, in situ, and online analysis of metabolites in complex biological samples has become a new requirement for the construction of environmental metabolomics and environmental toxicology.…”

Applications of ambient ionization mass spectrometry in 2022: An annual review

“…In a recent study utilizing reactive PS ionization, the authors were able to quickly explore different experimental parameters and reaction products by performing in situ reactions on the PS substrate. 162 The use of AIMS in environmental sciences has also exhibited some produced by cyanobacteria, in field samples, 164 the use of iEESI to detect perfluorooctanoic acid contamination in fish, 165 and the use of a dual plasma ion source (incorporating both corona discharge and a microwave plasma torch) to detect trace sterols in urban water samples. 166 Plasma-based AIMS techniques have also been utilized for the analysis of complex oil products.…”

“…Although a somewhat niche area of study, the research demonstrates the versatility of AIMS for environmental applications and the power of combining techniques to target a broader range of analytes. Other studies have further evaluated AIMS for different applications to environmental samples, including the use of DART to detect anatoxin‐a, a neurotoxin produced by cyanobacteria, in field samples, 164 the use of iEESI to detect perfluorooctanoic acid contamination in fish, 165 and the use of a dual plasma ion source (incorporating both corona discharge and a microwave plasma torch) to detect trace sterols in urban water samples. 166 …”