Internal and external quality control in the medical microbiology laboratory

Fowotade, A; Fayemiwo, S.A.; Bongomin, Felix; Fasuyi, T.O.; Aigbovo, O.A.; Adegboro, B

doi:10.4314/ajcem.v19i4.1

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…3,4 Quality control testing of platelets and other blood products in microbiology using bacterial culture and bacterial identification methodologies have been implemented in the blood transfusion field as a further mitigating practice. 5 These screening methodologies have shown effectiveness and have decreased events of transfusion-transmitted bacterial infections due to early detection of bacteria in contaminated products. 6,7 Rapid classification and detection of microbes is essential for the prevention of treatment failures and rapid spread of antimicrobial drug resistance.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a stable proficiency testing matrix in transfusion microbiology in South Africa

Mpumlwana,

Kruger,

Jentsch

2023

African Journal of Laboratory Medicine

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Background All medical laboratories must participate in proficiency testing (PT) programmes to ensure high-quality results. Proficiency testing samples mimic clinical samples; however, PT programmes for detection of bacteria in blood products are not routinely performed due to unavailability of matrix-equivalent samples. Objective The aim of this study was to develop and test a matrix-equivalent PT programme using blood products as the basis matrix. Methods A prospective cross-sectional study was conducted from April 2021 until June 2021, using 52 blood products comprising 36 pooled platelet and 16 red blood cell products at the South African National Blood Service PT laboratory in Gauteng. Products were manipulated into matrix-equivalent PT samples by spiking 42 products with known bacterial strains at specific concentrations and treating the remaining 10 products with preserving fluid containing antibiotics. The level of agreement between the researcher results and participating laboratories’ results was assessed. Results Of the prepared matrices, 568 out of 572 (99%) were stable for 30 days. Bacteria could correctly be identified in spiked samples for up to 23 days. Samples treated with preserving fluid remained negative until day 30. For spiked samples, an average of 98% agreement (153/156) was achieved between the three participating laboratories when compared with the researcher’s results; 100% agreement was achieved for unspiked samples. The kappa scores obtained from all tested variables presented with scores between 0.856 and 1.000, and the p -value was < 0.001 throughout. Conclusion The developed PT matrix was therefore stable and suitable to be implemented in transfusion microbiology. What this study adds This study demonstrated that a stable microbiology PT programme using platelets and red blood cells can be developed for use on bacterial detection analysers and could help to close the gap presented by unavailability of a blood PT matrix for transfusion microbiology.

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Section: Introductionmentioning

confidence: 99%

Establishment of a stable proficiency testing matrix in transfusion microbiology in South Africa

Mpumlwana,

Kruger,

Jentsch

2023

African Journal of Laboratory Medicine

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The influence of the microwave oven on the production of solid culture medium and quality of microbial growth

Bazana

Carvalho

Mace

2022

An. Acad. Bras. Ciênc.

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Numerous cultivation media currently exist, whether selective, non-selective, enrichment or identifi cation. However, they all have a common goal, which is the growth of microorganisms; the constitution and quality of the culture medium must favor it. For this reason, an important factor that directly affects the quality of a culture medium is its production. Thus, this article investigated the use of a microwave oven in the production of Sabouraud dextrose agar (SDA), and the microbial inactivation compared to the autoclave in a microbiology laboratory. The quality of the medium, time exposure, and sterilization potential were performed using fungal strains of Candida spp., Cryptococcus spp., Microsporum spp., and Aspergillus spp. The results showed that the advantages of the use of a microwave oven for the preparation of SDA are practicality, speed, lower energy expense, pH, and constituents preservation of the culture medium, resulting in a richer growth compared to autoclaved SDA. The multivariate analysis of digital images allowed the detection of melanoidins (brownish tone of medium), which are responsible for the negative infl uence on the microorganisms growth. This research shows the use of the microwave oven as an effi cient alternative for the production of the culture medium and maintaining their best quality.

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Internal and external quality control in the medical microbiology laboratory

Cited by 2 publications

References 13 publications

Establishment of a stable proficiency testing matrix in transfusion microbiology in South Africa

Establishment of a stable proficiency testing matrix in transfusion microbiology in South Africa

The influence of the microwave oven on the production of solid culture medium and quality of microbial growth

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