The influence of the microwave oven on the production of solid culture medium and quality of microbial growth

Bazana, Luana Candice Genz; Carvalho, Alberito Rodrigo de; Mace, Manoela

doi:10.1590/0001-3765202220211104

Cited by 4 publications

(12 citation statements)

References 33 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The result of this mapping is given in Figure 9: with a horizontal-axis of 0 to 30 kJ, and a vertical-axis of 0 to 10 kJ•m −1 . The comparison thermal data corresponds to the 39 L Sharp microwave oven S630D rated at 1050 ± 50 W CW [16], and the 28 L Electrolux microwave oven ME28S rated at 900 W CW [51]. The ≥4 log 10 inactivation microorganisms are Gram-negative vegetative E. coli, bacteriophage MS2 [16] and Candida spp.…”

Section: Energy Phase-space Projection and Electric Field Strength An...mentioning

confidence: 99%

“…The ≥4 log 10 inactivation microorganisms are Gram-negative vegetative E. coli, bacteriophage MS2 [16] and Candida spp. agar plated Petri dish [51].…”

Section: Energy Phase-space Projection and Electric Field Strength An...mentioning

confidence: 99%

“…American Journal of Analytical Chemistry ); Gram-negative vegetative E. coli 10.93 ml kitchen scrubbing pad (dotted-line) [16], 64 ml Gram-negative vegetative E. coli and bacteriophage MS2 sponge () [16], and 25 ml Candida spp. agar suspensions in Petri dish (star) [51].…”

Section: Energy Phase-space Projection and Electric Field Strength An...mentioning

confidence: 99%

“…Estimated ε ′′ , σ and E rms for Candida. app [51]. Figure 10 shows the suspension temperature (horizontal-axis) plotted against ε ′′ (vertical-axis), where the 5 point trend-line equates to y = 0.461x + 8.799 with an R 2 = 0.9933.…”

Section: Electric Field Strength Estimation Within the Energy Phase-s...mentioning

confidence: 99%

“…In 2022, Bazana et al [51] published a study on batch thermal microwave-assisted inactivation of Candida spp. in a 25 ml agar plated Petri dish.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Revisiting “Non-Thermal” Batch Microwave Oven Inactivation of Microorganisms

Law¹,

Dowling²

2023

AJAC

View full text Add to dashboard Cite

Over the last few decades there has been active discussion concerning the mechanisms involved in "non-thermal" microwave-assisted inactivation of microorganisms. This work presents a novel non-invasive acoustic measurement of a domestic microwave oven cavity-magnetron operating at f o = 2.45 ± 0.05 GHz (λ o ~ 12.2 cm) that is modulated in the time-domain (0 to 2 minutes). The measurements reveal the cavity-magnetron cathode filament cold-start warm-up period and the pulse width modulation periods (time-on time-off and base-time period, where time-on minus base-time = duty cycle). The waveform information is used to reconstruct historical microwave "nonthermal" homogeneous microorganism inactivation experiments: where tapwater is used to mimic the microorganism suspension; and ice, crushed ice, and ice slurry mixture are used as the cooling media. The experiments are described using text, diagrams, and photographs. Four key experimental parameters are indentified that influence the suspension time-dependent temperature profile. First, where the selected process time > the time-base, the cavity-magnetron continuous wave rated power should be used for each second of microwave illumination. Second, external crushed ice and ice slurry baths induce different cooling profiles due to difference in their heat absorption rates. In addition external baths may shield the suspension resulting in a retarding of the time-dependent heating profile. Third, internal cooling systems dictate that the suspension is directly exposed to microwave illumination due to the absence of surrounding ice volume. Fourth, four separated water dummyloads isolate and control thermal heat transfer (conduction) to and from the suspension, thereby diverting a portion of the microwave power away from the suspension. Energy phase-space projections were used to compare the "non-thermal" energy densities of 0.03 to 0.1 kJ⋅m −1 at 800 W with reported thermal microwave-assisted microorganism inactivation energy densities of 0.

show abstract

Section: Energy Phase-space Projection and Electric Field Strength An...mentioning

confidence: 99%

“…The ≥4 log 10 inactivation microorganisms are Gram-negative vegetative E. coli, bacteriophage MS2 [16] and Candida spp. agar plated Petri dish [51].…”

Section: Energy Phase-space Projection and Electric Field Strength An...mentioning

confidence: 99%

Section: Energy Phase-space Projection and Electric Field Strength An...mentioning

confidence: 99%

Section: Electric Field Strength Estimation Within the Energy Phase-s...mentioning

confidence: 99%

“…In 2022, Bazana et al [51] published a study on batch thermal microwave-assisted inactivation of Candida spp. in a 25 ml agar plated Petri dish.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Revisiting “Non-Thermal” Batch Microwave Oven Inactivation of Microorganisms

Law¹,

Dowling²

2023

AJAC

View full text Add to dashboard Cite

show abstract

Description of Dellaglioa carnosa sp. nov., a novel species isolated from high-oxygen modified-atmosphere packaged meat

Werum

Ehrmann

2023

Systematic and Applied Microbiology

View full text Add to dashboard Cite

Microwave Oven Application for the Preparation and Sterilization of Microbiological Culture Media: A Feasible Method with An Adapted Water Bath and Perforable Cap

Terrones-Fernandez,

Rodero-De-Lamo,

López

et al. 2024

Applied Sciences

View full text Add to dashboard Cite

There is currently increased interest in the use of alternatives to autoclaved culture media, in order to maintain the properties of the media, while saving energy and time. In this study, we assess a new system for culture media preparation, using a conventional microwave with a water bath and a glass bottle with a rubber cap that allows depressurization. Sterilization, using the proposed system (1000 W, 3 to 20 min), was compared with autoclaving for the preparation of tryptone soy agar (TSA), tryptone soy broth (TSB), Sabouraud 4% dextrose agar (SDA), and violet red bile glucose agar (VRBG). Microwave exposure for 7 min yielded sterile TSA plates. The productivity of both sterilization methods was assessed using the pour plate method, and significant increases in the growth of certain micro-organisms after using a microwave were observed for every culture medium, especially those that were sterilized by boiling (VRBG). The kinetics of microbial destruction showed that Escherichia coli and Bacillus subtilis spores were destroyed after 3 and 7 min in a microwave, respectively, while three decimal reductions were obtained for Geobacillus stearothermophilus spores after 15 min in an autoclave. This new sterilization method could be a feasible, rapid, and economical method to prepare microbiological media, with a quality similar to that obtained through autoclaving.

show abstract

The influence of the microwave oven on the production of solid culture medium and quality of microbial growth

Cited by 4 publications

References 33 publications

Revisiting “Non-Thermal” Batch Microwave Oven Inactivation of Microorganisms

Revisiting “Non-Thermal” Batch Microwave Oven Inactivation of Microorganisms

Description of Dellaglioa carnosa sp. nov., a novel species isolated from high-oxygen modified-atmosphere packaged meat

Microwave Oven Application for the Preparation and Sterilization of Microbiological Culture Media: A Feasible Method with An Adapted Water Bath and Perforable Cap

Contact Info

Product

Resources

About