Intermittent Straining Accelerates the Development of Tissue Properties in Engineered Heart Valve Tissue

Rubbens, M.P.; Mol, Anita; Boerboom, R.A.; Bank, Ruud A.; Baaijens, Fpt Frank; Bouten, Cvc Carlijn

doi:10.1089/ten.tea.2007.0396

Cited by 63 publications

(64 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Next, these constructs were strained cyclically for 7 days, using a previously established intermittent strain protocol 27 with uniaxial direction (Fig. 1, protocol B).…”

Section: Cyclic Strain Protocolmentioning

confidence: 99%

“…31,35 Alternatively, they have turned to use mechanical conditioning of the construct-typically consisting of (cyclic) straining regimens-to enhance tissue organization and mechanical properties. 2, 16,23,27 Although this has resulted in engineered tissues with improved collagen architecture and load-bearing properties, control over tissue remodeling during cyclic straining of samples with complex geometries has not yet been achieved. This is mainly due to our limited understanding of mechanically-induced collagen remodeling at the micro scale.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Strain-induced Collagen Organization at the Micro-level in Fibrin-based Engineered Tissue Constructs

et al. 2012

View full text Add to dashboard Cite

Full understanding of strain-induced collagen organization in complex tissue geometries to create tissues with predefined collagen architecture has not been achieved. This is mainly due to our limited knowledge of collagen remodeling in developing tissues. Here we investigate straininduced collagen (re)organization in fibrin based engineered tissues using nondestructive time-lapse imaging. The tissues start from a biaxially constrained myofibroblast-populated fibrin gel and are used to study: (A) remodeling from a static equi-biaxial loading condition to static uniaxial loading; and (B) remodeling of a biaxially constrained tissue under uniaxial cyclic straining before and after a change in strain direction. Under static conditions, collagen oriented parallel to the direction of strain, whereas under cyclic conditions the orientation in the constrained tissue was perpendicular to the direction of strain. It is concluded that due to the biaxial constraints the uniaxially, cyclically strained cells can exert forces in two directions and strain shield themselves. A subsequent change in the direction of cyclic straining resulted in a rapid reorientation of collagen at the tissue surface. Reorientation was significantly slower in deeper tissue layers, where tissue remodeling was dominated by contact guidance provided by the endogenous matrix. These findings emphasize the relevance of achieving a functional collagen organization right from the start of tissue culture.

show abstract

“…Next, these constructs were strained cyclically for 7 days, using a previously established intermittent strain protocol 27 with uniaxial direction (Fig. 1, protocol B).…”

Section: Cyclic Strain Protocolmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Strain-induced Collagen Organization at the Micro-level in Fibrin-based Engineered Tissue Constructs

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Figure 1 at the beginning of the paper shows the results on a microscopy image of collagen fibres, where two rotated version of the image have been superimposed for the sake of illustration of our algorithm. These kind of images are acquired in tissue engineering research, where the goal is to create artificial heart valves (Rubbens et al 2008). All parameters during these experiments were set the same as the artificial images mentioned above except for CED para- Fig.…”

Section: Coherence-enhancing Diffusion In Orientation Scoresmentioning

confidence: 99%

Crossing-Preserving Coherence-Enhancing Diffusion on Invertible Orientation Scores

2009

View full text Add to dashboard Cite

Many image processing problems require the enhancement of crossing elongated structures. These problems cannot easily be solved by commonly used coherenceenhancing diffusion methods. Therefore, we propose a method for coherence-enhancing diffusion on the invertible orientation score of a 2D image. In an orientation score, the local orientation is represented by an additional third dimension, ensuring that crossing elongated structures are separated from each other. We consider orientation scores as functions on the Euclidean motion group, and use the group structure to apply left-invariant diffusion equations on orientation scores. We describe how we can calculate regularized left-invariant derivatives, and use the Hessian to estimate three descriptive local features: curvature, deviation from horizontality, and orientation confidence. These local features are used to adapt a nonlinear coherence-enhancing, crossing-preserving, diffusion equation on the orientation score. We propose two explicit finite-difference schemes to apply the nonlinear diffusion in the orientation score and provide a stability analysis. Experiments on both artificial and medical images show that preservation of crossings is the main advantage compared to standard coherenceenhancing diffusion. The use of curvature leads to improved enhancement of curves with high curvature. Furthermore,

show abstract

“…The static control exhibited lower ECM production and no orientation in agreement with our previous comparative study on the influence of dynamic conditioning on the in vitro development of fibrin-based heart valves 33 and results presented by other groups as well. [55][56][57] With the preliminary results shown here, it is not possible to conclude if there is a clear difference between the two approaches. The optimization of both protocols with respect to tissue formation is subject of ongoing research.…”

Section: Weber Et Almentioning

confidence: 60%

Tissue-Engineered Fibrin-Based Heart Valve with a Tubular Leaflet Design

Weber

Heta

Moreira

et al. 2014

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

The general approach in heart valve tissue engineering is to mimic the shape of the native valve in the attempt to recreate the natural haemodynamics. In this article, we report the fabrication of the first tissue-engineered heart valve (TEHV) based on a tubular leaflet design, where the function of the leaflets of semilunar heart valves is performed by a simple tubular construct sutured along a circumferential line at the root and at three single points at the sinotubular junction. The tubular design is a recent development in pericardial (nonviable) bioprostheses, which has attracted interest because of the simplicity of the construction and the reliability of the implantation technique. Here we push the potential of the concept further from the fabrication and material point of view to realize the tube-in-tube valve: an autologous, living HV with remodelling and growing capability, physiological haemocompatibility, simple to construct and fast to implant. We developed two different fabrication/conditioning procedures and produced fibrin-based constructs embedding cells from the ovine umbilical cord artery according to the two different approaches. Tissue formation was confirmed by histology and immunohistology. The design of the tube-in-tube foresees the possibility of using a textile coscaffold (here demonstrated with a warp-knitted mesh) to achieve enhanced mechanical properties in vision of implantation in the aortic position. The tube-in-tube represents an attractive alternative to the conventional design of TEHVs aiming at reproducing the valvular geometry.

show abstract

Intermittent Straining Accelerates the Development of Tissue Properties in Engineered Heart Valve Tissue

Cited by 63 publications

References 33 publications

Strain-induced Collagen Organization at the Micro-level in Fibrin-based Engineered Tissue Constructs

Strain-induced Collagen Organization at the Micro-level in Fibrin-based Engineered Tissue Constructs

Crossing-Preserving Coherence-Enhancing Diffusion on Invertible Orientation Scores

Tissue-Engineered Fibrin-Based Heart Valve with a Tubular Leaflet Design

Contact Info

Product

Resources

About