Intermittent Parathyroid Hormone Administration Improves Periodontal Healing in Rats

Vasconcelos, Daniel Fernando Pereira; Marques, Marcelo Rocha; Benatti, Bruno Braga; Barros, Silvana P.; Nociti, Francisco Humberto; Novaes, Pedro Duarte

doi:10.1902/jop.2013.130155

Cited by 30 publications

(41 citation statements)

References 51 publications

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“…The cementogenesis- and differentiation-related biomarkers BSP, OCN, COL1 and Osx were also stimulated by intermittent PTH. Previous in vivo studies showed that PTH administration increased cementum width in an osteoporotic rabbit model or improved the formation of newly formed cementum-like tissue in a rat model [18, 19]. Therefore, these findings together suggest that intermittent PTH can be a potential therapeutic agent for root resorption.…”

Section: Discussionmentioning

confidence: 76%

Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts

Zhou

et al. 2017

BMC Cell Biol

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BackgroundExternal root resorption, commonly starting from cementum, is a severe side effect of orthodontic treatment. In this pathological process and repairing course followed, cementoblasts play a significant role. Previous studies implicated that parathyroid hormone (PTH) could act on committed osteoblast precursors to promote differentiation, and inhibit apoptosis. But little was known about the role of PTH in cementoblasts. The purpose of this study was to investigate the effects of intermittent PTH on cementoblasts and its influence after mechanical strain treatment.ResultsHigher levels of cementogenesis- and differentiation-related biomarkers (bone sialoprotein (BSP), osteocalcin (OCN), Collagen type I (COL1) and Osterix (Osx)) were shown in 1–3 cycles of intermittent PTH treated groups than the control group. Additionally, intermittent PTH increased alkaline phosphatase (ALP) activity and mineralized nodules formation, as measured by ALP staining, quantitative ALP assay, Alizarin red S staining and quantitative calcium assay. The morphology of OCCM-30 cells changed after mechanical strain exertion. Expression of BSP, ALP, OCN, osteopontin (OPN) and Osx was restrained after 18 h mechanical strain. Furthermore, intermittent PTH significantly increased the expression of cementogenesis- and differentiation-related biomarkers in mechanical strain treated OCCM-30 cells.ConclusionsTaken together, these data suggested that intermittent PTH promoted cementum formation through activating cementogenesis- and differentiation-related biomarkers, and attenuated the catabolic effects of mechanical strain in immortalized cementoblasts OCCM-30.Electronic supplementary materialThe online version of this article (doi:10.1186/s12860-017-0133-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 76%

Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts

Zhou

et al. 2017

BMC Cell Biol

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“…Micro‐CT and histological observations demonstrated that the systemic intermittent PTH improved bone formation in periodontal defects at 2 weeks and 4 weeks postoperative. Previously, it was shown that intermittent PTH treatment improved alveolar bone levels affected by periodontitis in rats and humans (Barros et al, ; Bashutski et al, ; Marques et al, ; Vasconcelos et al, ). The cyclic adenosine monophosphate (cAMP)–protein kinase signalling pathway response to PTH 1 receptor (PTH1R) activation is considered to be the main mechanism that mediates the anabolic action of PTH in bone (Swarthout, D’Alonzo, Selvamurugan, & Partridge, ; Yang, Guo, Divieti, & Bringhurst, ).…”

Section: Discussionmentioning

confidence: 98%

“…The administration of PTH was performed essentially as described by Vasconcelos et al (). Animals in the PTH group received intermittent subcutaneous injections of recombinant human PTH (1–34) (Sigma‐Aldrich) at 40 µg/kg every 2 days for 2 weeks or 4 weeks.…”

Section: Methodsmentioning

confidence: 99%

“…It has been reported for rat femurs that intermittent PTH therapy is better at increasing bone mass than continuous PTH therapy (Hock & Gera, ). Furthermore, data from animal studies (Barros, Silva, Somerman, & Nociti, ; Tokunaga et al, ; Vasconcelos et al, ) and human studies in patients with periodontitis (Bashutski et al, ) showed that intermittent PTH treatment results in improved bone healing.…”

Section: Introductionmentioning

confidence: 99%

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Combined effects of systemic parathyroid hormone (1–34) and locally delivered neutral self‐assembling peptide hydrogel in the treatment of periodontal defects: An experimental in vivo investigation

Yoshida

Matsugami

Murakami

et al. 2019

J Clinic Periodontology

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Aim To evaluate in vivo combination therapy of systemic parathyroid hormone (PTH) and locally delivered neutral self‐assembling peptide (SAP) hydrogel for periodontal treatment. Materials and Methods Viability/proliferation of rat periodontal ligament cells in a neutral SAP nanofibre hydrogel (SPG‐178) was evaluated using WST‐1 assay. Periodontal defects were created mesially to the maxillary first molars in 40 Wistar rats. Defects were filled with 1.5% SPG‐178 or left unfilled. Animals received PTH (1‒34) or saline injections every 2 days. Microcomputed tomography, histological, and immunohistochemical examinations were used to evaluate healing at 2 or 4 weeks postoperative. Results At 72 hr, cells in 1.5% SPG‐178 showed increased viability/proliferation compared to cells in 0.8% SPG‐178 or untreated controls. In vivo, systemic PTH resulted in significantly greater bone volume in the Unfilled group at 2 weeks (p = .01) and 4 weeks (p < .0001) than in the saline control. At 4 weeks, a significantly greater bone volume was observed in the PTH/SPG‐178 (p = .0003) and PTH/Unfilled (p = .004) groups than in Saline/SPG‐178 group. Histologically, greater bone formation was observed in PTH/SPG‐178 at 4 weeks than in other groups. In the PTH/SPG‐178 group, increased proportions of PCNA‐, VEGF‐, and Osterix‐positive cells were observed in the treated sites. Conclusions These findings suggest that intermittent systemic PTH and locally delivered neutral SAP hydrogel enhance periodontal healing.

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“…, Vasconcelos et al. ) and human (Bashutski et al. ) experiments have shown that intermittent administration of PTH or its recombinant form – teriparatide – results in less alveolar bone loss, a reduced number of inflammatory cells in the inflamed gingival area, and improved bone healing.…”

mentioning

confidence: 99%

Serum parathyroid hormone and active vitamin D in chronic periodontitis

Antonoglou

Knuuttila

Niemelä³

et al. 2015

J Clinic Periodontology

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Intermittent Parathyroid Hormone Administration Improves Periodontal Healing in Rats

Abstract: Within the limits of the present study, intermittent administration of hPTH 1-34 led to an enhanced periodontal healing process compared with non-treated animals.

Cited by 30 publications

References 51 publications

Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts

Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts

Combined effects of systemic parathyroid hormone (1–34) and locally delivered neutral self‐assembling peptide hydrogel in the treatment of periodontal defects: An experimental in vivo investigation

Serum parathyroid hormone and active vitamin D in chronic periodontitis

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