Combined effects of systemic parathyroid hormone (1–34) and locally delivered neutral self‐assembling peptide hydrogel in the treatment of periodontal defects: An experimental in vivo investigation

Yoshida, Wataru; Matsugami, Daisuke; Murakami, Tasuku; Bizenjima, Takahiro; Imamura, Kentaro; Seshima, Fumi; Saitô, Atsushi

doi:10.1111/jcpe.13170

Cited by 19 publications

(11 citation statements)

References 47 publications

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“…Furthermore, the CT in P 11 -4-treated defects appeared healthier, with a high condensation of newly formed blood capillaries which promote wound healing ( Guo and DiPietro, 2010 ). Our results are consistent with the findings of others using different SAPs, who concluded that SAP nanofiber scaffolds accelerate wound healing and re-epithelialization ( Schneider et al, 2008 ; Meng et al, 2009 ; Yoshida et al, 2019 ).…”

Section: Discussionsupporting

confidence: 93%

An in-vivo Intraoral Defect Model for Assessing the Use of P11-4 Self-Assembling Peptide in Periodontal Regeneration

El-Sayed

Davies

El-Zehery

et al. 2020

Front. Bioeng. Biotechnol.

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Periodontal disease is one of the most common diseases worldwide. It has a significant impact on oral health and subsequently the individual's quality of life. However, optimal regeneration of periodontal tissues, using current treatments, has yet to be achieved. Peptide self-assembly has provided a step-change in nanobiotechnology and regenerative medicine fields. Our aim was to investigate the effects of a selfassembling peptide (SAP; P 11-4) on periodontal regeneration in a preclinical model. Twenty-six bilateral maxillary critical-sized periodontal defects were created surgically in 13 rats. Defects on one side of the mouth were filled with P 11-4 hydrogel; the contralateral defect was untreated (control). Rats were sacrificed immediately post-surgery (time 0) and after 2 and 4 weeks. Retrieved maxillae were processed for histological, immunohistochemical, and histomorphometric assessments. The results of histological analysis showed greater organization of periodontal fibers in defects treated with P 11-4, at both time points, when compared to untreated defects. Histomorphometry showed that treated defects had both a significant increase in functional periodontal ligament length and a reduction in epithelial down growth after 4 weeks. At 2 weeks, treated defects showed a significant increase in expression of osteocalcin and osteoprotegerin as judged by immunohistochemistry. Also, a significantly higher osteoprotegerin/RANKL ratio was shown in treated defects. In conclusion, the results demonstrated enhanced regeneration of periodontal tissues when SAP P 11-4 was used to fill periodontal defects in rats. The findings of this study suggest that SAP P 11-4 is a promising novel candidate for periodontal regenerative therapy. Further investigations are required for optimization before clinical use.

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Section: Discussionsupporting

confidence: 93%

An in-vivo Intraoral Defect Model for Assessing the Use of P11-4 Self-Assembling Peptide in Periodontal Regeneration

El-Sayed

Davies

El-Zehery

et al. 2020

Front. Bioeng. Biotechnol.

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“…For the observation of PCNA-, VEGF-, and Osx-positive cells, magnification was set at ×200, and a field of connective tissue (350 µm × 470 µm area) was randomly sampled in each section and submitted to quantitative analysis using an image analyzer (Image-Pro Plus 6.2, Media Cybernetics, Rockville, MD, USA), essentially as described previously [31]. On each specimen, analysis sites were compartmentalized into three areas (Root side, Bone side, and Middle area) to count PCNA-, VEGF-, or Osx-positive cells [24,32].…”

Section: Immunohistochemistrymentioning

confidence: 99%

Healing of Experimental Periodontal Defects Following Treatment with Fibroblast Growth Factor-2 and Deproteinized Bovine Bone Mineral

et al. 2021

Self Cite

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The aim of this study was to investigate the effects of fibroblast growth factor (FGF)-2 used in combination with deproteinized bovine bone mineral (DBBM) on the healing of experimental periodontal defects. Periodontal defects created in rats were treated by FGF-2, DBBM, FGF-2 + DBBM, or left unfilled. Microcomputed tomography, histological, and immunohistochemical examinations were used to evaluate healing. In vitro cell viability/proliferation on DBBM with/without FGF-2 was assessed by WST-1. Cell behavior was analyzed using scanning electron and confocal laser scanning microscopy. Osteogenic differentiation was evaluated by staining with alkaline phosphatase and alizarin red. Bone volume fraction was significantly greater in FGF-2 and FGF-2 + DBBM groups than in other groups at 2 and 4 weeks postoperatively. In histological assessment, newly formed bone in FGF-2 and FGF-2 + DBBM groups appeared to be greater than other groups. Significantly greater levels of proliferating cell nuclear antigen-, vascular endothelial growth factor-, and osterix-positive cells were observed in FGF-2 and FGF-2 + DBBM groups compared to Unfilled group. In vitro, addition of FGF-2 to DBBM promoted cell viability/proliferation, attachment/spreading, and osteogenic differentiation. The combination therapy using FGF-2 and DBBM was similarly effective as FGF-2 alone in the healing of experimental periodontal defects. In certain bone defect configurations, the combined use of FGF-2 and DBBM may enhance healing via promotion of cell proliferation, angiogenesis, and osteogenic differentiation.

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“…To assess the effect of different concentrations of M101 on EC metabolic activity, an AlamarBlue assay (Life Technologies, Saint-Aubin, France) was performed as described previously 27 . Twenty-four hours before the experiment, 2 × 10 5 EC were seeded in each well of a 24-well plate.…”

Section: Methodsmentioning

confidence: 99%

A therapeutic oxygen carrier isolated from Arenicola marina decreased P. gingivalis induced inflammation and tissue destruction

Batool

Stutz

Petit

et al. 2020

Sci Rep

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The control of inflammation and infection is crucial for periodontal wound healing and regeneration. M101, an oxygen carrier derived from Arenicola marina, was tested for its anti-inflammatory and anti-infectious potential based on its anti-oxidative and tissue oxygenation properties. In vitro, no cytotoxicity was observed in oral epithelial cells (EC) treated with M101. M101 (1 g/L) reduced significantly the gene expression of pro-inflammatory markers such as TNF-α, NF-κΒ and RANKL in P. gingivalis-LPS stimulated and P. gingivalis-infected EC. The proteome array revealed significant down-regulation of pro-inflammatory cytokines (IL-1β and IL-8) and chemokine ligands (RANTES and IP-10), and upregulation of pro-healing mediators (PDGF-BB, TGF-β1, IL-10, IL-2, IL-4, IL-11 and IL-15) and, extracellular and immune modulators (TIMP-2, M-CSF and ICAM-1). M101 significantly increased the gene expression of Resolvin-E1 receptor. Furthermore, M101 treatment reduced P. gingivalis biofilm growth over glass surface, observed with live/dead analysis and by decreased P. gingivalis 16 s rRNA expression (51.7%) (p < 0.05). In mice, M101 reduced the clinical abscess size (50.2%) in P. gingivalis-induced calvarial lesion concomitant with a decreased inflammatory score evaluated through histomorphometric analysis, thus, improving soft tissue and bone healing response. Therefore, M101 may be a novel therapeutic agent that could be beneficial in the management of P. gingivalis associated diseases.

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Combined effects of systemic parathyroid hormone (1–34) and locally delivered neutral self‐assembling peptide hydrogel in the treatment of periodontal defects: An experimental in vivo investigation

Cited by 19 publications

References 47 publications

An in-vivo Intraoral Defect Model for Assessing the Use of P11-4 Self-Assembling Peptide in Periodontal Regeneration

An in-vivo Intraoral Defect Model for Assessing the Use of P11-4 Self-Assembling Peptide in Periodontal Regeneration

Healing of Experimental Periodontal Defects Following Treatment with Fibroblast Growth Factor-2 and Deproteinized Bovine Bone Mineral

A therapeutic oxygen carrier isolated from Arenicola marina decreased P. gingivalis induced inflammation and tissue destruction

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