Intermediate filaments in cell migration and invasion: the unusual suspects

Leduc, Cécile; Etienne-Manneville, Sandrine

doi:10.1016/j.ceb.2015.01.005

Cited by 121 publications

(107 citation statements)

References 84 publications

(61 reference statements)

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“…Because IFs associate with microtubules (Leduc and Etienne-Manneville, 2015), we investigated by RNAi whether the microtubule-associated RhoA exchange factor GEF-H1 (Ren et al, 1998; Krendel et al, 2002) could mediate the cross-talk between vimentin and RhoA signaling during stress fiber formation and contractility. With appropriate siRNA oligonucleotide, we succeeded in efficiently depleting GEF-H1 from U2OS cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA

Jiu

Peränen

Schaible

et al. 2017

Journal of Cell Science

155

161

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The actin and intermediate filament cytoskeletons contribute to numerous cellular processes, including morphogenesis, cytokinesis and migration. These two cytoskeletal systems associate with each other, but the underlying mechanisms of this interaction are incompletely understood. Here, we show that inactivation of vimentin leads to increased actin stress fiber assembly and contractility, and consequent elevation of myosin light chain phosphorylation and stabilization of tropomyosin-4.2 (see Geeves et al., 2015). The vimentin-knockout phenotypes can be rescued by re-expression of wild-type vimentin, but not by the non-filamentous ‘unit length form’ vimentin, demonstrating that intact vimentin intermediate filaments are required to facilitate the effects on the actin cytoskeleton. Finally, we provide evidence that the effects of vimentin on stress fibers are mediated by activation of RhoA through its guanine nucleotide exchange factor GEF-H1 (also known as ARHGEF2). Vimentin depletion induces phosphorylation of the microtubule-associated GEF-H1 on Ser886, and thereby promotes RhoA activity and actin stress fiber assembly. Taken together, these data reveal a new mechanism by which intermediate filaments regulate contractile actomyosin bundles, and may explain why elevated vimentin expression levels correlate with increased migration and invasion of cancer cells.

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Section: Resultsmentioning

confidence: 99%

Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA

Jiu

Peränen

Schaible

et al. 2017

Journal of Cell Science

155

161

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“…Since actin cytoskeleton rearrangement is required for cell migration [32,33], we examined actin morphology after rhodamine-phalloidin staining of Swan 71 cells incubated 30 min or 9 h in the presence or absence of 100 ng/ml of G-CSF. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation

Furmento

Marino

Blank

et al. 2016

Experimental Cell Research

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Multiple cytokines and growth factors expressed at the fetal-maternal interface are involved in the regulation of trophoblast functions and placental growth, but the role of G-CSF has not been completely established. Based on our previous study showing that G-CSF increases the activity of matrix metalloproteinase-2 and the release of vascular endothelial growth factor in Swan 71 human trophoblast cells, in this work we explore the possible contribution of G-CSF to cell migration and the G-CSF-triggered signaling pathway. We found that G-CSF induced morphological changes on actin cytoskeleton consistent with a migratory cell phenotype. G-CSF also up-regulated the expression levels of β1 integrin and promoted Swan 71 cell migration. By using selective pharmacological inhibitors and dominant negative mutants we showed that PI3K, Erk 1/2 and p38 pathways are required for promoting Swan 71 cell motility. It was also demonstrated that PI3K behaved as an upstream regulator of Erk 1/2 and p38 MAPK. In addition, the increase of β1 integrin expression was dependent on PI3K activation. In conclusion, our results indicate that G-CSF stimulates β1 integrin expression and Swan 71 cell migration by activating PI3K and MAPK signaling pathways, suggesting that G-CSF should be considered as an additional regulatory factor that contributes to a successful embryo implantation and to the placenta development.

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“…In contrast, higher vimentin expression promotes cell migration (e.g. during epithelial mesenchymal transition) [56]. Moreover, desmin has been implicated in smooth muscle contraction and cell migration [57, 58].…”

Section: Role Of Intermediate Filaments In Cell Migrationmentioning

confidence: 99%

“…Intermediate filaments have been shown to physically link to focal contacts in the protrusion of motile cells [56]. This raises the possibility that IFs may regulate focal adhesion dynamics directly, and thus cell migration.…”

Section: Role Of Intermediate Filaments In Cell Migrationmentioning

confidence: 99%

The roles and regulation of the actin cytoskeleton, intermediate filaments and microtubules in smooth muscle cell migration

2017

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Smooth muscle cell migration has been implicated in the development of respiratory and cardiovascular systems; and airway/vascular remodeling. Cell migration is a polarized cellular process involving a protrusive cell front and a retracting trailing rear. There are three cytoskeletal systems in mammalian cells: the actin cytoskeleton, the intermediate filament network, and microtubules; all of which regulate all or part of the migrated process. The dynamic actin cytoskeleton spatially and temporally regulates protrusion, adhesions, contraction, and retraction from the cell front to the rear. c-Abl tyrosine kinase plays a critical role in regulating actin dynamics and migration of airway smooth muscle cells and nonmuscle cells. Recent studies suggest that intermediate filaments undergo reorganization during migration, which coordinates focal adhesion dynamics, cell contraction, and nucleus rigidity. In particular, vimentin intermediate filaments undergo phosphorylation and reorientation in smooth muscle cells, which may regulate cell contraction and focal adhesion assembly/disassembly. Motile cells are characterized by a front-rear polarization of the microtubule framework, which regulates all essential processes leading to cell migration through its role in cell mechanics, intracellular trafficking, and signaling. This review recapitulates our current knowledge how the three cytoskeletal systems spatially and temporally modulate the migratory properties of cells. We also summarize the potential role of migration-associated biomolecules in lung and vascular diseases.

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Intermediate filaments in cell migration and invasion: the unusual suspects

Cited by 121 publications

References 84 publications

Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA

Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA

Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation

The roles and regulation of the actin cytoskeleton, intermediate filaments and microtubules in smooth muscle cell migration

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