Interleukin Production by Human Colostral Cells After In Vitro Mitogen Stimulation

Abstract

Human colostral cells were pulsed with PHA, Con A, or LPS and cultivated in serum-free medium. The culture supernatants were tested for IL-1 activity in C3H/HeJ thymocyte assay and for IL-2 activity on human lymphoblasts. The IL-1 activity was the highest at the 24th h of cultivation and IL-2 activity at the 48th h of cultivation.

The Role of Human Milk Immunomodulators in Protecting Against Viral Bronchiolitis and Development of Chronic Wheezing Illness

“…The lymphocyte population of human milk consisting largely of T cells is made up of significantly more activated (HLA-DR + and CD25 + ), and memory (CD45RO + ) T cells than peripheral blood [ 70 , 71 , 72 , 73 ]. These lymphocytes also produce a number of cytokines ex vivo [ 74 , 75 ]. The cell surface molecules CD30 and CD40 ligand (CD40-L), both members of the TNF receptor family, are upregulated on colostral T cells compared to peripheral blood T cells [ 76 , 77 ].…”

Human Colostrum Stimulates Cytokine Production

“…The in vivo functions of the stimulatory activ ities for cytokine production observed in hu man milk are unknown. These can act on the mononuclear cells present in the milk [26][27][28] which have been shown to have the capacity to respond to immunogenic stimulation [29][30] and to produce cytokines such as IL-1 [31][32][33], IL-2 [31] and interferon-y [33], Alter natively, the stimulatory activities for cyto kine production may act on the oropharyngeal and intestinal-associated lymphoid tissues. From these sites activated lymphocytes and monocytes can influence other distant lym phoid and nonlymphoid tissues thus inducing immunostimulation.…”