Interleukin 8 (IL-8) - a universal biomarker?

Shahzad, Aamir; Knapp, Martin; Lang, Irene M.; Köhler, Gabriele

doi:10.1186/1755-7682-3-11

Cited by 101 publications

(74 citation statements)

References 18 publications

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“…37 IL-8 mRNA is stable in many cell lines and cancer cells as reported here and by others. If in some cell types a sustained stable IL-8 mRNA is considered an aberration; the PMA/THP-1 differentiation system could be used as a simple and easy tool for in the depth molecular investigation of this novel form of sustained stabilization of a chemokine mRNA.…”

Section: Discussionsupporting

confidence: 49%

Sustained stabilization of Interleukin-8 mRNA in human macrophages

et al. 2014

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Section: Discussionsupporting

confidence: 49%

Sustained stabilization of Interleukin-8 mRNA in human macrophages

et al. 2014

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“…Cytokines are important mediators of inflammation and are usually associated with pathogenesis of many inflammatory diseases. Therefore, IL-8 is currently being applied as noninvasive diagnostic marker in various fields of medicine either for the purpose of early diagnosis or as a prognosis predictor [22]. Furthermore, recently, Ray et al [23] has identified 18 signaling proteins in blood plasma that can be used to classify and predict clinical Alzheimer's diagnosis, among which IL-8 has been also identified.…”

Section: ) Nacl Solution Measurementsmentioning

confidence: 99%

Wireless Point-of-Care Platform With Screen-Printed Sensors for Biomarkers Detection

Tonello

Abate

Borghetti

et al. 2017

IEEE Trans. Instrum. Meas.

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Abstract-Measurement systems for early and reliable detection of degenerative diseases, such as Alzheimer's disease (AD), are extremely important in clinical diagnosis. Among these, biochemical assays represent a commonly used method to distinguish patients from healthy population thanks to the sensitive recognition of specific biomarkers in biological fluids. In order to overcome actual limitations of these techniques in term of cost, standardization, and sensitivity, this study aimed to realize a low-cost highly sensitive portable point-of-care (PoC) testing system based on screen-printed electrochemical sensors. The development of the platform specifically included both the design of the sensing probe and the electronic circuit devoted to condition and acquires the transduced electric signal. The designed circuit was implemented in a printed circuit board and interfaced to a wireless system based on bluetooth data transmission in order to improve the portability of the proposed solution. Preliminary results were obtained by using controlled concentrations of electrolytic solutions and calibrating the sensors for antibodies and for a well-known protein (i.e., interleukin 8) quantified by anodic stripping voltammetry (ASV). Findings from ASV measurements showed a sensitivity of 38 µA/(ng/ml) with a tested range from 1.25 to 20 ng/ml, with a limit of detection of 2 ng/ml. Further investigation will include the validation of this PoC device by testing the concentration of a specific p53 protein isoform, which was recently identified to early correlate to AD development.

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“…Secreted proteins play an important role in determination, control, and coordination of biological processes such as cell growth, differentiation, immune defense, and pathological processes such as cancer progression [1,2]. Comparing the secretome of cells in a normal as well as in a stressed situation, for example induced by inflammation or other disease states, may thus allow determining alterations which are characteristic for any given state [3].…”

Section: Introductionmentioning

confidence: 99%

Quantification of cytokines secreted by primary human cells using multiple reaction monitoring: evaluation of analytical parameters

et al. 2015

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Determination of secreted proteins provides highly valuable information about cell functions. While the typical methods for the determination of biologically relevant but low abundant molecular species still rely on the use of specific antibodies, mass spectrometry-based methods are now gaining sufficient sensitivity to cope with such challenges as well. In the current study, we have identified several cytokines and chemokines which were induced in primary human umbilical vein endothelial cells upon inflammatory activation. Based on the high-resolution mass spectrometry data obtained with a Q Exactive orbitrap, we built an MRM method to quantify the most relevant molecules selected from the screening experiment. All experimental data are available via ProteomeXchange, PXD002211/12, and Panorama ( www.panoramaweb.org ). Using nano-flow Chip-HPLC coupled to a 6490 triple-quadrupole MS for MRM analyses, we achieved calibration curves covering a linear range of four orders of magnitude and detection limits in the low attomol per microliter concentration range. Carryover was consistently less than 0.005 %, the accuracy was between 80 and 120 %, and the median coefficient of variation for LC/MS was only 2.2 %. When including the variance of quantification introduced by cell culture and digestion, the coefficient of variation was less than 20 % for most peptides. With appropriate marker molecules, we monitored typical variations introduced by cell culture caused by differences in cell numbers, proliferative states, and cell death. As a result, here, we present a robust and efficient MRM-based assay for the accurate and sensitive determination of cytokines and chemokines representative for functional cell states and including comprehensive quality controls. Graphical Abstract Work flow diagram: Data processing steps beginning with orbitrap-based shotgun data acquisition and MaxQuant data analysis, followed by peptide and transition selection for MRM analysis using Skyline and experimental validation using triple quadrupole MS.

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Interleukin 8 (IL-8) - a universal biomarker?

Cited by 101 publications

References 18 publications

Sustained stabilization of Interleukin-8 mRNA in human macrophages

Sustained stabilization of Interleukin-8 mRNA in human macrophages

Wireless Point-of-Care Platform With Screen-Printed Sensors for Biomarkers Detection

Quantification of cytokines secreted by primary human cells using multiple reaction monitoring: evaluation of analytical parameters

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