Interleukin-8 Expression by Head and Neck Squamous Cell Carcinoma

Cohen, Richard F.; Contrino, Josephine; Spiro, Jeffrey D.; Mann, Eric A.; Chen, Lei L.; Kreutzer, Donald L.

doi:10.1001/archotol.1995.01890020064013

Cited by 85 publications

(41 citation statements)

References 21 publications

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“…GRO-a is an autocrine growth factor identi®ed in melanoma (Balentien et al, 1991), and is a member of the C-X-C family of chemokines which promote chemotaxis of granulocytes and endothelia (Strieter et al, 1996). We and others have found that human SCC also produce C-X-C chemokines with potential proin¯ammatory and proangiogenic activity, including IL-8 Cohen et al, 1995;Smith et al, 1994) and GRO-a (Loukinova, unpublished results). mGRO-a shares major sequence homology and leukocyte chemotactic activity (Oquendo et al, 1989;Bozic et al, 1995) with both human GRO-a and IL-8, for which independent murine counterparts have not been identi®ed.…”

Section: Introductionmentioning

confidence: 64%

Growth Regulated Oncogene-α expression by murine squamous cell carcinoma promotes tumor growth, metastasis, leukocyte infiltration and angiogenesis by a host CXC Receptor-2 dependent mechanism

et al. 2000

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Growth Regulated Oncogene-a (GRO-a) is an autocrine growth factor in melanoma and is a member of the C-X-C family of chemokines which promote chemotaxis of granulocytes and endothelia through binding to CXC Receptor 2. We found previously that variants of murine squamous cell carcinoma PAM 212 which grow and metastasize more rapidly in vivo constitutively express increased levels of murine GRO-a, designated mGRO-a, or KC. We have examined the possible role of mGRO-a expression in malignant progression of squamous cell carcinoma PAM 212 in homologous BALB/c and BALB CXC Receptor-2 de®cient mice. Transfection of the PAM 212 cell line which exhibits low expression of GRO-a and malignant potential with a pActin-KC vector encoding mGRO-a enabled isolation of PAM-KC expressing cell lines. These PAM-KC transfectants displayed an increased rate of growth and metastasis in BALB/c mice, similar to the highly malignant phenotype observed in spontaneously occurring metastatic variants. Furthermore, the PAM-KC tumors showed an increase in in®ltration of host leukocytes and CD31+ blood vessels, consistent with increased CXC chemokine activity. The increased growth of PAM-KC cells was attenuated in CXCR-2 de®cient mice, indicating that the increased growth was dependent in part upon host cells responsive to the CXC chemokine. Together, these results show that a CXC chemokine such as GRO-a can promote malignant growth of murine squamous cell carcinoma by a host CXCR-2 dependent pathway.

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Section: Introductionmentioning

confidence: 64%

Growth Regulated Oncogene-α expression by murine squamous cell carcinoma promotes tumor growth, metastasis, leukocyte infiltration and angiogenesis by a host CXC Receptor-2 dependent mechanism

et al. 2000

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“…In recent years, studies have shown that IL-8 is closely associated with the occurrence of tumors. The secretion and expression of IL-8 mRNA and protein have been detected in many tumor tissues and GA cell lines (Cohen et al, 1995;Brew et al, 1996;Yoshimura et al, 2002). Promoter regions of a number of cytokine genes contain polymorphisms that directly influence cytokine production (Bidwell et al, 2001).…”

Section: Lack Of Association Of a Common Polymorphism In The 3' -Utr mentioning

confidence: 99%

Lack of Association of a Common Polymorphism in the 3'-UTR of Interleukin 8 with Non Small Cell Lung Cancer in Kashmir

Bhat¹,

Pandith²,

Bhat³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…Among the repertoire of cytokines detected in HNSCC cells, IL-8 is a pro-in¯ammatory and proangiogenic cytokine consistently expressed at elevated concentrations in tumor specimens, cell lines, and serum from patients relative to controls [2,5,6], and the IL-8 promoter contains NF-kB, AP-1, and NF-IL6 binding sites that have been shown to promote inducible IL-8 gene expression in response to various stimuli [13,36]. To examine the functional importance of NF-kB, AP-1, and NF-IL6 sites present in the proximal region of the IL-8 promoter, we performed luciferase reporter gene assays in UM-SCC-9 cells by using plasmids that contain a wildtype 133-bp IL-8 promoter fragment or mutagenized NF-kB, AP-1, and NF-IL6 sites [36].…”

Section: Nf-kb and Ap-1 Sites In The Il-8 Promoter Involved In Cytokimentioning

confidence: 99%

Constitutive activation of transcription factors NF-?B, AP-1, and NF-IL6 in human head and neck squamous cell carcinoma cell lines that express pro-inflammatory and pro-angiogenic cytokines

et al. 1999

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We previously reported that human head and neck squamous cell carcinomas (HNSCCs) express the pro-inflammatory and pro-angiogenic cytokines interleukin (IL)-1alpha, IL-6, IL-8, and granulocyte-macrophage colony-stimulating factor in vitro and in vivo. The promoter region of the genes encoding these cytokines include binding sites for the transcription factors nuclear factor (NF) kappaB/Rel A, activator protein-1 (AP-1), and CCAAT enhancer-binding protein beta (C/EBPbeta, or NF-IL6), which have been reported to contribute to activation of these cytokine genes. In the study presented here, we examined the activation, composition, and function of these transcription factors in HNSCC cell lines that express pro-inflammatory cytokines, by using electrophoretic mobility shift and reporter-gene assays. Constitutive activation of NF-kappaB, AP-1, and NF-IL6 DNA-binding proteins was detected. Supershift analysis with antibodies specific for NF-kappaB, AP-1, and NF-IL6 binding proteins showed that the NF-kappaB-binding protein included p65/Rel A and p50; AP-1 activity included c-jun, junB, junD, and Fra-1; and NF-IL6 included C/EBPbeta. Mutational analysis of the NF-kappaB, AP-1, and NF-IL6 sites in the IL-8 promoter region showed that NF-kappaB and AP-1 sites contributed to constitutive IL-8 reporter activity in HNSCC. HNSCC lines that exhibited increased IL-8 secretion relative to simian virus 40-immortalized and primary keratinocyte cell lines also demonstrated a concordant increase in NF-kappaB reporter activity relative to nonmalignant keratinocytes. We concluded that the early transcription factors NF-kappaB, AP-1, and NF-IL6 are constitutively activated in human HNSCC cell lines and that NF-kappaB and AP-1 promote expression of the pro-inflammatory and pro-angiogenic cytokine IL-8 in HNSCC. The demonstration of the activation of these transcription factors will be helpful in defining the identity and role of these and other early gene products that contribute to pathogenesis of the malignant phenotype in HNSCC and in defining potential targets for pharmacologic and molecular therapy of HNSCC. Mol. Carcinog. 26:119-129, 1999. Published 1999 Wiley-Liss, Inc.

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Interleukin-8 Expression by Head and Neck Squamous Cell Carcinoma

Cited by 85 publications

References 21 publications

Growth Regulated Oncogene-α expression by murine squamous cell carcinoma promotes tumor growth, metastasis, leukocyte infiltration and angiogenesis by a host CXC Receptor-2 dependent mechanism

Growth Regulated Oncogene-α expression by murine squamous cell carcinoma promotes tumor growth, metastasis, leukocyte infiltration and angiogenesis by a host CXC Receptor-2 dependent mechanism

Lack of Association of a Common Polymorphism in the 3'-UTR of Interleukin 8 with Non Small Cell Lung Cancer in Kashmir

Constitutive activation of transcription factors NF-?B, AP-1, and NF-IL6 in human head and neck squamous cell carcinoma cell lines that express pro-inflammatory and pro-angiogenic cytokines

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