Lack of Association of a Common Polymorphism in the 3'-UTR of Interleukin 8 with Non Small Cell Lung Cancer in Kashmir

Bhat, Imtiyaz Ahmad; Pandith, Arshid A.; Bhat, Bashir Ahmad; Naykoo, Niyaz A.; Qasim, Iqbal; Rasool, Roohi; Aziz, Sheik Aejaz; Shah, Zafar A.

doi:10.7314/apjcp.2013.14.7.4403

Cited by 12 publications

(8 citation statements)

References 37 publications

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“…When we investigated the IL-8-845 T/C polymorphism, our results revealed an association of IL-8-845 C with susceptibility to gastric cancer and the influence of the C allele on the IL-8 mRNA expression level. The scarcity of studies on this polymorphism may be explained by the low frequency of the C allele, which is absent in European and Asian populations [19,57]. In contrast, this allele is common in individuals of African descent (19 %) [58], with a frequency similar to that of gastric cancer patients in our study (15 %).…”

Section: Discussioncontrasting

confidence: 60%

Influence of functional polymorphisms in TNF-α, IL-8, and IL-10 cytokine genes on mRNA expression levels and risk of gastric cancer

et al. 2015

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Functional polymorphisms in promoter regions can produce changes in the affinity of transcription factors, thus altering the messenger ribonucleic acid (mRNA) expression levels of inflammatory cytokines associated with the risk of cancer development. The goal of this study was to evaluate the influence that polymorphisms in the cytokine genes known as TNF-α-308 G/A (rs1800629), TNF-α-857 C/T (rs1799724), IL-8-251 T/A (rs4073), IL-8-845 T/C (rs2227532), and IL-10-592 C/A (rs1800872) have on changes to mRNA expression levels and on the risks of chronic gastritis (CG) and gastric cancer (GC). A sample of 723 individuals was genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Relative mRNA expression levels were measured using quantitative real-time PCR (qPCR). Polymorphisms TNF-α-308 G/A and IL-8-251 A/T were not associated with risks of these gastric lesions. However, TNF-α-857 C/T, IL-8-845 T/C, and IL-10-592 C/A were found to be associated with a higher risk of GC, and IL-10-592 C/A was found to be associated with a higher risk of CG. The relative mRNA expression levels (RQ) of TNF-α, IL-8, and IL-10 were markedly downregulated in the CG group (median RQs = 0.128, 0.247, and 0.614, respectively), while the RQ levels of TNF-α in the GC group were upregulated (RQ = 2.749), but were basal for IL-8 (RQ = 1.053) and downregulated for IL-10 (RQ = 0.179). When the groups were stratified according to wild-type and polymorphic alleles, only for IL-8-845 T/C the polymorphic allele was found to influence the expression levels of this cytokine. IL-8-845 C allele carriers were significantly upregulated in both groups (GC and CG; RQ = 3.138 and 2.181, respectively) when compared to TT homozygotes (RQ = -0.407 and 0.165, respectively). In silico analysis in the IL-8 promoter region revealed that the presence of the variant C allele in position -845 is responsible for the presence of the binding sites for two transcription factors (REL and CREB1), which are involved in increased gene expression. Polymorphic alleles were not shown to have any effect on the expression levels of TNF-α and IL-10. Taken together, our findings provide evidence for an association of TNF-α-857 C/T, IL-8-845 T/C, and IL-10-592 C/A with a higher risk of gastric cancer and also demonstrate the influence that the polymorphic C allele of IL-8-845 has on changes to the gene expression levels of this cytokine.

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Section: Discussioncontrasting

confidence: 60%

Influence of functional polymorphisms in TNF-α, IL-8, and IL-10 cytokine genes on mRNA expression levels and risk of gastric cancer

et al. 2015

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“…In the Indian context, polymorphisms in XRCC1, p53, N-acetylation 2, EGFR, and UGT genes were reported to augment lung cancer susceptibility [29]. However, there are no reports that correlate the association of cytokine gene polymorphisms and NSCLC risk in the Indian population, except for one report that suggests a dearth of association between the IL-8 gene polymorphism and NSCLC in the population of Kashmir valley [30]. The Indian subcontinent is diverse in terms of climate, food habits, lifestyle, environmental factors, and, more importantly, the ethnic background of the population.…”

Section: Introductionmentioning

confidence: 97%

Association of TNFα−308, IFNγ+874, and IL10−1082 gene polymorphisms and the risk of non-small cell lung cancer in the population of the South Indian state of Telangana

Peddireddy

Badabagni

Sulthana³

et al. 2016

Int J Clin Oncol

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“…Lung carcinogenesis is a complex process requiring the acquisition of genetic mutations that confer the malignant phenotype as well as epigenetic alterations (Bhat et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Prognostic Value of Methylation Status and Expression Levels of FHIT, GSTP1 and p16 in Non-Small Cell Lung Cancer in Egyptian Patients

Haroun

Zakhary²,

Mohamed³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Lack of Association of a Common Polymorphism in the 3'-UTR of Interleukin 8 with Non Small Cell Lung Cancer in Kashmir

Cited by 12 publications

References 37 publications

Influence of functional polymorphisms in TNF-α, IL-8, and IL-10 cytokine genes on mRNA expression levels and risk of gastric cancer

Influence of functional polymorphisms in TNF-α, IL-8, and IL-10 cytokine genes on mRNA expression levels and risk of gastric cancer

Association of TNFα−308, IFNγ+874, and IL10−1082 gene polymorphisms and the risk of non-small cell lung cancer in the population of the South Indian state of Telangana

Assessment of the Prognostic Value of Methylation Status and Expression Levels of FHIT, GSTP1 and p16 in Non-Small Cell Lung Cancer in Egyptian Patients

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