Interleukin-6 Stimulates Cell Migration, Invasion and Integrin Expression in HTR-8/SVneo Cell Line

Jovanović, Milica; Vićovac, Ljiljana

doi:10.1016/j.placenta.2009.01.013

Cited by 118 publications

(90 citation statements)

References 46 publications

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“…Jovanovic and Vicovac (2009) reported that both endogenous IL-6 and exogenous IL-6 increased the migration and invasive potential of the trophoblast-like cell line HTR-8/SVneo due to the up-regulation of the trophoblast integrin receptors α1, α5 and β5, receptors which bind to components within the extracellular matrix. However, Champion et al (2012) reported no effect on EVT invasion from first trimester placental explants.…”

Section: Il-6mentioning

confidence: 99%

Decidual cytokines and pregnancy complications: focus on spontaneous miscarriage

Lash

Ernerudh

2015

Journal of Reproductive Immunology

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The establishment of pregnancy requires the co-ordinated implantation of the embryo into the receptive decidua, placentation, trophoblast invasion of the maternal decidua and myometrium in addition to remodelling of the uterine spiral arteries. Failure of any of these steps can lead to a range of pregnancy complications, including miscarriage, pre-eclampsia, fetal growth restriction, placenta accreta and pre-term birth. Cytokines are small multifunctional proteins often derived from leucocytes and have primarily been described through their immunomodulatory actions. The maternal-fetal interface is considered to be immunosuppressed to allow development of the semi-allogeneic placental fetal unit. However, cytokine profiles of the decidua and different decidual cell types suggest that the in vivo situation might be more complex. Data suggest that decidual-derived cytokines not only play roles in immunosuppression, but also in other aspects of the establishment of pregnancy, including the regulation of trophoblast invasion and spiral artery remodelling. This review focuses on the potential role of decidua-derived cytokines in the aetiology of unexplained spontaneous miscarriage. (C) 2015 Elsevier Ireland Ltd. All rights reserved

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Section: Il-6mentioning

confidence: 99%

Decidual cytokines and pregnancy complications: focus on spontaneous miscarriage

Lash

Ernerudh

2015

Journal of Reproductive Immunology

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“…Increases in a 5 and b 1 integrin proteins were found in HTR-8/SVneo cells treated with IL8. Other cytokines and growth factors influencing trophoblast invasion, such as epidermal growth factor or IL6, were also reported to affect integrin expression (Leach et al 2004, Jovanović & Vićovac 2009). …”

Section: Il8 Increases Trophoblast Mmps and Integrinsmentioning

confidence: 99%

“…Various autocrine and paracrine factors have been suggested to modulate trophoblast function (reviewed by Salamonsen et al (2007)). Many cytokines are produced by both trophoblast and diverse decidual cell types, and some, such as tumor necrosis factor-a (TNF; Bauer et al 2004), interleukin-1 (IL1; Librach et al 1994), interferon-g (Lash et al 2006), leukemia inhibitory factor (LIF; Poehlmann et al 2005), IL6 (Jovanović & Vićovac 2009), and transforming growth factor-b (TGFB; Caniggia et al 1999, Lash et al 2005, are implicated in the regulation of trophoblast invasion.…”

Section: Introductionmentioning

confidence: 99%

Interleukin-8 (CXCL8) stimulates trophoblast cell migration and invasion by increasing levels of matrix metalloproteinase (MMP)2 and MMP9 and integrins α5 and β1

Jovanović¹,

Stefanoska²,

Radojčić³

et al. 2010

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Interleukin-8 (IL8/CXCL8) is present in decidua and trophoblast, which also expresses the IL8 receptors, CXCR1 and CXCR2. IL8 was shown to stimulate trophoblast migration. Matrix metalloproteinase (MMP)2, MMP9, and integrins a 5 b 1 and a 1 b 1 were found to play important roles in trophoblast invasion. We hypothesized that IL8 would increase this cell migration and invasion by HTR-8/SVneo cells through the activity of MMPs and integrins. Isolated first trimester of pregnancy cytotrophoblast (CT) and HTR-8/SVneo cell line were used. Migration was studied by monolayer wounding test, and invasion by Matrigel invasion test. The effects of IL8 on MMPs and integrin subunit expression were determined in HTR-8/SVneo cells by gelatin zymography and western blot respectively. The results that were obtained showed that exogenous IL8 stimulated HTR-8/SVneo cell migration and invasion. MMP2 and MMP9 levels were stimulated to 182% (P!0.01) and 134% (P!0.01) respectively. Integrin a 5 expression was increased to 119% (P!0.05) and integrin b 1 expression to 173% (P!0.001) of the control values. The data that were obtained show for the first time the sensitivity of the HTR-8/SVneo cells, in addition to isolated first trimester CT, to IL8. Exogenous IL8/CXCL8 increased trophoblast cell migration and invasion, which may be partly attributable to stimulation of MMP2 and MMP9 levels and an increase in integrins. HTR-8/SVneo cell viability and proliferation were also increased.

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“…It has been proposed that cytokines may promote neovascularization and attachment of endometrial cells to the peritoneum in the process of development of endometriosis [1] [4] [7]- [11].…”

Section: Introductionmentioning

confidence: 99%

TNF<i>α</i> and IL1<i>β</i> Stimulate Differential Gene Expression in Endometrial Stromal Cells

Chalpe¹,

Law²,

Dumdie

et al. 2015

ABC

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The purpose of this study was to test the hypothesis that specific macrophage-secreted cytokines cause gene expression changes in endometrial stromal cells that reproduce the effects of macrophages in the development of endometriosis. Telomerase-immortalized human endometrial stromal cells (T-HESC) were treated with tumor necrosis factor α (TNFα, 5 ng/ml) and interleukin 1β (IL1β, 1 ng/ml). Differential expression of 249 genes was identified by DNA microarray. Ontologies such as peptidases, cell adhesion, cell death/cell cycle, growth factors, cytoskeletal organization, defense/immune system, signal transduction, and transcriptional regulation which are related to the development of endometriosis were represented by these genes. The up-regulation of interleukin 8 (IL8), interleukin 6 (IL6), IL1β and matrix metalloproteinase 3 (MMP3) in response to TNFα ± ILIβ in T-HESC cells was confirmed by real time RT-PCR. TNFα ± ILIβ did not affect the migration or invasion of T-HESC cells. This study reinforces our previous investigations on communication between cells of the immune system and endometrial stromal cells and their potential role in the development of endometriosis.

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Interleukin-6 Stimulates Cell Migration, Invasion and Integrin Expression in HTR-8/SVneo Cell Line

Cited by 118 publications

References 46 publications

Decidual cytokines and pregnancy complications: focus on spontaneous miscarriage

Decidual cytokines and pregnancy complications: focus on spontaneous miscarriage

Interleukin-8 (CXCL8) stimulates trophoblast cell migration and invasion by increasing levels of matrix metalloproteinase (MMP)2 and MMP9 and integrins α5 and β1

TNF<i>α</i> and IL1<i>β</i> Stimulate Differential Gene Expression in Endometrial Stromal Cells

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Interleukin-6 Stimulates Cell Migration, Invasion and Integrin Expression in HTR-8/SVneo Cell Line

Cited by 118 publications

References 46 publications

Decidual cytokines and pregnancy complications: focus on spontaneous miscarriage

Decidual cytokines and pregnancy complications: focus on spontaneous miscarriage

Interleukin-8 (CXCL8) stimulates trophoblast cell migration and invasion by increasing levels of matrix metalloproteinase (MMP)2 and MMP9 and integrins α5 and β1

TNF&lt;i&gt;α&lt;/i&gt; and IL1&lt;i&gt;β&lt;/i&gt; Stimulate Differential Gene Expression in Endometrial Stromal Cells

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TNF<i>α</i> and IL1<i>β</i> Stimulate Differential Gene Expression in Endometrial Stromal Cells