TNF&amp;lt;i&amp;gt;α&amp;lt;/i&amp;gt; and IL1&amp;lt;i&amp;gt;β&amp;lt;/i&amp;gt; Stimulate Differential Gene Expression in Endometrial Stromal Cells

Chalpe, Abha J.; Law, C.; Dumdie, Jennifer N; Hansen, Keith A.; Eyster, Kathleen M.

doi:10.4236/abc.2015.52010

Cited by 5 publications

(5 citation statements)

References 29 publications

(34 reference statements)

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“…Similar finding was also reported by Chalpe et al. () that telomerase‐immortalized human endometrial stromal cells (T‐HESC) treated with TNF α did not enhance the gene expression of CXCL8/IL8, IL‐1 β , and IL6. TNF α did, however, significantly induce chemokines CCL2, CCL20, CCL5, CX3CL1, and CXCL5 expression in bovine endometrial stromal cells, similar to the results of a study by Liu et al.…”

Section: Discussionsupporting

confidence: 89%

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Regulation of inflammatory mediator expression in bovine endometrial cells: effects of lipopolysaccharide, interleukin 1 beta, and tumor necrosis factor alpha

et al. 2018

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An abnormal uterine environment can influence maternal–fetal communication, conception rate and disrupt normal embryo development, thereby affecting fertility and the reproductive performance of dairy cows. Animal variability means that development of endometrial cell lines with appropriate characteristic are required. We evaluated the effect of an infectious agent (i.e., bacterial lipopolysaccharide; LPS) and proinflammatory mediators (i.e., Interleukin 1 beta; IL‐1β, and tumor necrosis factor alpha; TNFα) on inflammatory mediator gene expression and production by bovine endometrial epithelial (bEEL) and stromal (bCSC) cell lines. Expression of CXCL8/IL8, IL1A, IL1B, and IL6 cytokine genes was significantly upregulated in both epithelial and stromal cells when treated with LPS and IL‐1β. LPS treatment of epithelial cells (compared with treatment by IL‐1β and TNFα) exhibited greater CXCL8/IL8, IL1A, IL1B, and IL6 cytokine gene expression. Whereas, in stromal cells, IL‐1β treatment (compared with LPS and TNFα) exhibited greater CXCL8/IL8, IL1A, IL1B, and IL6 cytokine gene expression. Interestingly, bEEL and bCSC cells treated with IL‐1β increased IL1B gene expression, suggesting that IL‐1β may act unusually in an autocrine‐positive feedback loop. Cytokine production was stimulated by these agents in both cell types. We suggest that the characteristics of these two cell lines make them excellent tools for the study of intrauterine environment.

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Section: Discussionsupporting

confidence: 89%

“…; IL1B , and IL6 – Chalpe et al. ), chemokines ( CCL20, CXCL3, CXCL5 – Rossi et al. , and GRO1 – Nasu et al.…”

Section: Discussionmentioning

confidence: 99%

Regulation of inflammatory mediator expression in bovine endometrial cells: effects of lipopolysaccharide, interleukin 1 beta, and tumor necrosis factor alpha

et al. 2018

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“…In the previous studies, it was demonstrated that some pro-inf lammatory genes including IL-1 and COX-2 can demonstrate a significantly dysregulated expression in some of tissues of endometriosis patients [10,12,[26][27][28]. For instance, Chalpe et al (2015) [26] demonstrated that pro-inf lammatory genes, including IL-1β induce differential expressions in endometrial stromal cells. Our results indicated that MenSCs-CM significantly inhibited the expression of IL-1β and COX-2 in endometriosis cells.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the effect of conditioned medium from endometrial stem cells on endometriosis-derived endometrial stem cells

et al. 2022

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Endometriosis is a common, benign gynecological disease which is determined as an overspreading of endometrial tissue in exterior region of the uterine cavity. Evidence suggests that retrograde menstrual blood which contains mesenchymal stem cells with differential gene expression compared to healthy women may play a role in endometriosis creation. We aimed to identify whether the conditioned medium (CM) from menstrual blood-derived mesenchymal stem cells (MenSCs) of healthy women can affect the expression level of inflammatory and stemness genes of MenSCs from endometriosis women. Endometriosis-derived MenSCs (E-MenSCs) were treated with CM derived from healthy women's MenSCs (non-endometriosis derived MenSCs [NE-MenSCs]). Some CD markers were analyzed by flow cytometer before and after treatment compared with NE-MenSCs, and the expression level of inflammatory and stemness genes was evaluated by real-time PCR. E-MenSCs show different morphology in vitro culture in comparison with NE-MenSCs, which were changed in the presence of CM, into a morphology more similar to normal cells and showed significant decrease expression of CD10 after CM treatment. In our results, the interleukin-1, cyclooxygenase-2, and hypoxia-inducible factor 1α as inflamaturay genes and octamer-binding transcription factor 4, NANOG, and sex determining region Y-box 2 as stemness genes showed significantly different expression level in E-MenSCs after treating with CM. Our study indicates that the expression level of some inflammatory-and stemness-related genes which have differential expression in E-MenSCs compared with NE-MenSCs, could be changed to normal status by using CM derived from NE-MenSCs.

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“…In the previous studies, it was demonstrated that some pro-in ammatory genes including IL-1 and COX-2 can demonstrate a signi cantly dysregulated expression in some of tissues of endometriosis patients [33,12,[34][35][36]. For instance, Chalpe et al (2015) demonstrated that pro-in ammatory genes, including IL-1β induce differential expressions in endometrial stromal cells [33].…”

Section: Discussionmentioning

confidence: 99%

Conditioned medium from healthy women's endometrial stem cells improve inflammatory and stemness- expression genes in endometriosis women

Sahraei

Kowsari

Asl

et al. 2021

Preprint

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Background. Endometriosis is a common, benign gynecological disease which is determined as an overspreading of endometrial tissue in exterior region of the uterine cavity. Evidence suggests that retrograde menstrual blood which contains mesenchymal stem cells with differential gene expression compared to healthy women may play a role in endometriosis creation. We aimed to identify whether the conditioned medium from Menstrual blood-derived stem cells (MenSCs) of healthy women can affect the expression level of inflammatory and stemness genes of MenSCs from endometriosis women. Methods and Results. Endometriosis derived MenSCs (E-MenSCs) were treated with conditioned medium (CM) derived from healthy women’s MenSCs (NE-MenSCs). Some CD markers were analyzed by flow cytometer before and after treatment compared with NE-MenSCs, and the expression level of inflammatory and stemness genes was evaluated by real-time PCR. Results. E-MenSCs show different morphology in vitro culture in comparison with NE-MenSCs, which were changed in the presence of CM, into a morphology more similar to normal cells and showed significant decrease expression of CD10 after CM treatment. In our results, the IL-1, COX-2, and HIF-1\(\alpha\) as an inflamaturay genes and OCT-4, NANOG, and SOX2 as a stemness genes showed significantly different expression level in E-MenSCs after treating with CM. Conclusions. Our study indicates that the expression level of some inflammatory- and stemness-related genes which have differential expression in E-MenSCs compared with NE-MenSCs, could be changed to normal status by using CM derived from NE-MenSCs.

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TNF<i>α</i> and IL1<i>β</i> Stimulate Differential Gene Expression in Endometrial Stromal Cells

Cited by 5 publications

References 29 publications

Regulation of inflammatory mediator expression in bovine endometrial cells: effects of lipopolysaccharide, interleukin 1 beta, and tumor necrosis factor alpha

Regulation of inflammatory mediator expression in bovine endometrial cells: effects of lipopolysaccharide, interleukin 1 beta, and tumor necrosis factor alpha

Evaluating the effect of conditioned medium from endometrial stem cells on endometriosis-derived endometrial stem cells

Conditioned medium from healthy women's endometrial stem cells improve inflammatory and stemness- expression genes in endometriosis women

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