Interleukin‑6 released by oral lichen planus myofibroblasts promotes angiogenesis

Xu, Xiaofei; Liu, Yang; Lu, Feng; Yang, Yueping; Liu, Shuguang; Guo, Wei; Hui-xi, Zhou; Li, Zhiqiang; Zhang, Lin; Meng, Wenxia

doi:10.3892/etm.2021.9722

Cited by 17 publications

(29 citation statements)

References 31 publications

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“…Interestingly, we found the expression of FAP-α in OLP AFs was signi cantly increased compared with NFs by real-time RT-PCR. However, there was no signi cant difference of FAP-α expression between the NEOLP and EOLP groups, which was consistent with previous reports [13,23] . This suggest that a subset of OLP-AFs acquire active phenotypes and may be involved in the pathogenesis of OLP.…”

Section: Discussionsupporting

confidence: 92%

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MiR-155-5p modulates inflammatory phenotype of activated oral lichen-planus-associated-fibroblasts by targeting SOCS1

Cheng¹,

Zhang²,

Liu³

et al. 2021

Preprint

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Micro (MiR)-155-5p plays a vital role in inflammation and immune responses, but the function in oral lichen planus (OLP) remains unclear. Recent research has revealed that OLP associated-fibroblasts (OLP AFs) contribute to the inflammatory process with activated phenotype. This study was carried out to assess the role of miR-155-5p in the pro-inflammatory process of activated OLP AFs. Normal mucosal fibroblasts (NFs) and OLP AFs were isolated from oral mucosa of healthy controls and OLP patients respectively. We detected the expression of miR-155-5p and FAP-α in NFs and OLP AFs by real-time RT-PCR and calculated their correlation. Enzyme-linked immunosorbent assay was used to detect cytokine production. Immunohistochemistry and western blotting assay were used to detect suppressor of cytokine signaling 1 (SOCS1) expression. Dual-luciferase reporter assay was conducted to explore the interaction between miR-155-5p and SOCS1. MiR-155-5p and FAP-α were significantly increased in OLP AFs, and positively correlated. The expression of SOCS1 is decreased in the epithelial layer of OLP tissues and OLP AFs. Overexpression of miR-155-5p augmented interleukin-6 (IL-6) and interleukin-8 (IL-8) release. The knockdown of miR-155-5p in OLP AFs decreased IL-6 and IL-8 release. Furthermore, miR-155-5p inhibits SOCS1 expression by directly targeting its 3′-UTR in NFs and OLP AFs. SOCS1 silencing augmented IL-6 and IL-8 production in NFs. Here, we suggest that miR-155-5p regulates the secretion of IL-6 and IL-8 by downregulating the expression of SOCS1 in activated OLP AFs. Our results provide novel insights into the pathogenesis of OLP and a new target for OLP future therapy.

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Section: Discussionsupporting

confidence: 92%

“…Previous studies demonstrate that OLP AFs can acquire activated phenotype and participate in local in ammatory and angiogenic processes. However, studies on OLP-AFs are still scarce [23] . Fibroblasts synthesize the extracellular matrix of connective tissue and play an essential role in wound healing and brosis [7,24] .…”

Section: Discussionmentioning

confidence: 99%

MiR-155-5p modulates inflammatory phenotype of activated oral lichen-planus-associated-fibroblasts by targeting SOCS1

Cheng¹,

Zhang²,

Liu³

et al. 2021

Preprint

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“…Silencing of MEG3 significantly reduced the myofibroblast activities, including migration and collagen gel contraction abilities and a-SMA and COL1A1 expression ( Figure 3 a–c, respectively). Myofibroblasts have been reported to promote tissue inflammation by stimulating interleukin (IL)-6 secretion, thus leading to fibrosis and cancer progression [ 42 , 43 , 44 , 45 ]. Silencing of MEG3 suppressed the secretion of TGF-β1 and IL-6 from both fBMFs ( Figure 3 d).…”

Section: Resultsmentioning

confidence: 99%

Fucoidan-Mediated Inhibition of Fibrotic Properties in Oral Submucous Fibrosis via the MEG3/miR-181a/Egr1 Axis

et al. 2022

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Oral submucous fibrosis (OSF) is a chronic fibrotic remodeling disease that can progress to oral cancer. However, efficient clinical diagnosis and treatment methods for OSF are still lacking. This study investigated the anti-fibrotic effect of fucoidan on oral fibrosis. To evaluate the fibrotic ability (myofibroblast activities), we performed wound-healing, Transwell migration, and collagen contraction assays by using patient-derived normal and fibrotic buccal submucous fibroblasts (BMFs and fBMFs, respectively). RNA-sequencing and dual-luciferase reporter and RNA immunoprecipitation chip assays were performed to identify the clinical significance and molecular mechanism of non-coding RNAs. Fucoidan suppressed the myofibroblast activities and inhibited the MEG3 in fBMFs. MEG3 was overexpressed in the OSF tissue and was positively associated with myofibroblast markers. Knockdown of MEG3 markedly inhibited myofibroblast activities, which were restored by inhibiting miR-181a and overexpressing Egr1. The results from luciferase reporter and RIP assays confirmed that MEG3 functioned as a competing endogenous RNA (ceRNA) and could directly target miR-181a, thereby preventing the miR-181a-mediated translational repression of Egr1. This study demonstrated that MEG3 exerts a profibrotic effect on OSF by targeting miR-181a/Egr1. Therefore, the administration of fucoidan may serve as a potential therapeutic strategy for OSF by targeting the overexpression of MEG3.

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“…In many studies and meta-analyses, they were highly expressed in OLP patient samples (mainly saliva and serum) compared to healthy controls [ 35 , 37 , 41 , 47 , 55 , 56 , 57 , 58 ]. The angiogenic process in OLP was increased by IL-6 [ 59 ], supporting its role in the pathogenesis of the disease [ 60 ] and, together with IL-8 and TNF-α, as a potential diagnostic and prognostic marker of malignant transformation [ 37 , 38 , 52 , 61 ]. While OLP saliva samples were more indicated for TNF-α and IL-6 monitoring [ 58 , 62 , 63 ], IL-8 levels were better detected in serum, being, in this case, a more sensitive marker than IL-6 to monitor the disease activity [ 64 ].…”

Section: Cytokines and Olpmentioning

confidence: 99%

Probiotics as Potential Biological Immunomodulators in the Management of Oral Lichen Planus: What’s New?

Zanetta

Ormelli

Amoruso

et al. 2022

IJMS

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Oral lichen planus (OLP) is a T cell-mediated chronic inflammatory disorder with multifactorial aetiology and malignant transformation potential. Despite the treatments so far identified, new tailored and safe specific measures are needed. Recently, human microbiota imbalance has been linked to several immune-mediated diseases, opening new therapeutic perspectives for probiotics; besides their ability to directly interact with the host microbiota, they also display a strain-specific immune-modulatory effect. Thus, this non-systematic review aims to elucidate the molecular pathways underlying probiotic activity, mainly those of Lactobacilli and Bifidobacteria and their metabolites in OLP pathogenesis and malignant transformation, focusing on the most recent in vitro and in vivo research evidence. Findings related to their activity in other immune-mediated diseases are here included, suggesting a probiotic translational use in OLP. Probiotics show immune-modulatory and microbiota-balancing activities; they protect the host from pathogens, hamper an excessive effector T cell response, reduce nuclear factor-kappa B (NF-kB) signalling and basal keratinocytes abnormal apoptosis, shifting the mucosal response towards the production of anti-inflammatory cytokines, thus preventing uncontrolled damage. Therefore, probiotics could be a highly encouraging prevention and immunotherapeutic approach for a safer and more sustainable OLP management.

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Interleukin‑6 released by oral lichen planus myofibroblasts promotes angiogenesis

Cited by 17 publications

References 31 publications

MiR-155-5p modulates inflammatory phenotype of activated oral lichen-planus-associated-fibroblasts by targeting SOCS1

MiR-155-5p modulates inflammatory phenotype of activated oral lichen-planus-associated-fibroblasts by targeting SOCS1

Fucoidan-Mediated Inhibition of Fibrotic Properties in Oral Submucous Fibrosis via the MEG3/miR-181a/Egr1 Axis

Probiotics as Potential Biological Immunomodulators in the Management of Oral Lichen Planus: What’s New?

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