Interleukin-6 negatively regulatesPorphyromonas gingivalisfimbria-stimulated fibronectin expression in human gingival fibroblasts

Abstract

Our previous study demonstrated that fibronectin (FN) is a negative regulator of Porphyromonas gingivalis fimbria-induced pathogenesis in the initiation and development of chronic periodontal diseases. We show herein the regulatory action of interleukin-6 (IL-6) on FN expression in fimbria-treated human gingival fibroblasts. Interestingly, the decrease in FN expression in the cells treated with fimbriae at a high dose (8 microg of protein ml(-1)) was negated by treatment with anti-IL-6 antibody. Also, the increase in FN expression in cells treated with fimbriae at a low dose (1 microg of protein ml(-1)) was inhibited by exogenous IL-6. These results suggest that P. gingivalis fimbria-stimulated FN expression in human gingival fibroblasts is negatively regulated by endogenous IL-6.

Porphyromonas gingivalis Fimbria-Induced Expression of Inflammatory Cytokines and Cyclooxygenase-2 in Mouse Macrophages and Its Inhibition by the Bioactive Compounds Fibronectin and Melatonin

“…Moreover, Kawata et al have observed that fimbria-stimulated bone resorption is inhibited by pretreatment with FN [35]. In addition, we have observed that FN is negatively regulated by fimbria-stimulated endogenous interleukin-6 and that the amount of FN in gingival crevicular fluid is decreased in patients with severe periodontal disease [36]. These observations suggest that FN might play a functional role as a negative regulator of fimbria-mediated pathogenesis in the initiation and development of chronic periodontal disease.…”