An ortho dimer of butylated hydroxyanisole inhibits nuclear factor kappa B activation and gene expression of inflammatory cytokines in macrophages stimulated by Porphyromonas gingivalis fimbriae

Murakami, Yukio; Shoji, Masao; Hirata, Atsushi; Tanaka, Shoji; Hanazawa, Shigemasa; Yokoe, Ichiro; Fujisawa, Seiichiro

doi:10.1016/j.abb.2006.02.005

Cited by 6 publications

(5 citation statements)

References 26 publications

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“…However, the capacity of FimA to subsequently elicit an inflammatory response is controversial. Some research groups have shown that FimA induces the production of pro-inflammatory cytokines from mononuclear innate cells [34], [45] and have hypothesized that this contributes to the development of pathological inflammation [21], [44], [46]. However, other studies suggest that the pro-inflammatory response to FimA in mononuclear innate cells may be significantly lower than that expected from comparable doses of classic TLR agonists [22] and is unlikely to play a major role in P. gingivalis -induced inflammation [47].…”

Section: Discussionmentioning

confidence: 99%

Tobacco Upregulates P. gingivalis Fimbrial Proteins Which Induce TLR2 Hyposensitivity

et al. 2010

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BackgroundTobacco smokers are more susceptible to periodontitis than non-smokers but exhibit reduced signs of clinical inflammation. The underlying mechanisms are unknown. We have previously shown that cigarette smoke extract (CSE) represents an environmental stress to which P. gingivalis adapts by altering the expression of several virulence factors – including major and minor fimbrial antigens (FimA and Mfa1, respectively) and capsule – concomitant with a reduced pro-inflammatory potential of intact P. gingivalis.Methodology/Principal FindingsWe hypothesized that CSE-regulation of capsule and fimbrial genes is reflected at the ultrastructural and functional levels, alters the nature of host-pathogen interactions, and contributes to the reduced pro- inflammatory potential of smoke exposed P. gingivalis. CSE induced ultrastructural alterations were determined by electron microscopy, confirmed by Western blot and physiological consequences studied in open-flow biofilms. Inflammatory profiling of specific CSE-dysregulated proteins, rFimA and rMfa1, was determined by quantifying cytokine induction in primary human innate and OBA-9 cells. CSE up-regulates P. gingivalis FimA at the protein level, suppresses the production of capsular polysaccharides at the ultrastructural level, and creates conditions that promote biofilm formation. We further show that while FimA is recognized by TLR2/6, it has only minimal inflammatory activity in several cell types. Furthermore, FimA stimulation chronically abrogates the pro-inflammatory response to subsequent TLR2 stimulation by other TLR-2-specific agonists (Pam3CSK4, FSL, Mfa1) in an IκBα- and IRAK-1-dependent manner.Conclusions/SignificanceThese studies provide some of the first information to explain, mechanistically, how tobacco smoke changes the P. gingivalis phenotype in a manner likely to promote P. gingivalis colonization and infection while simultaneously reducing the host response to this major mucosal pathogen.

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Section: Discussionmentioning

confidence: 99%

Tobacco Upregulates P. gingivalis Fimbrial Proteins Which Induce TLR2 Hyposensitivity

et al. 2010

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“…We previously investigated the antioxidative and anti-inflammatory activity of some natural and synthetic phenols, which are NSAID-like compounds possessing high antioxidative activity. We found that, in particular, ortho -dimer phenols, bis -BHA (3,3′-di-t-butyl-5,5′-dimethoxy-1,1′-biphenyl-2,2′diol), showed good radical-scavenging activity and also inhibited Pg fimbria-stimulated inflammatory cytokine expression and NF- κ B activation in RAW264.7 cells [68]. In addition, we have confirmed that magnolol and/or honokiol, which are hydroxylated biphenyl compounds isolated from Magnolia officinalis , inhibit the fimbria-stimulated COX-2 expression resulting from NF- κ B activation in the same cells (data not shown).…”

Section: Melatoninmentioning

confidence: 99%

Porphyromonas gingivalis Fimbria-Induced Expression of Inflammatory Cytokines and Cyclooxygenase-2 in Mouse Macrophages and Its Inhibition by the Bioactive Compounds Fibronectin and Melatonin

2012

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Porphyromonas gingivalis(Pg) fimbriae, in addition to lipopolysaccharide, are involved in the pathogenesis of periodontal disease. At the same time, bioactive compounds such as fibronectin (FN) and melatonin in saliva and gingival crevicular fluid have been reported to exert a preventive effect against periodontitis. Here, we review current knowledge regarding the potent inhibitory effects of FN and melatonin against Pg fimbria-induced induction of proinflammatory cytokines, cyclooxygenase-2 (COX-2) expression, and NF-kappa B activation in mouse macrophages and discuss their possible clinical application for prevention of periodontal diseases induced by oral bacteria.

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“…Dimers were synthesized, and their radical-scavenging and biological activities were compared with those of the original or other phenols. Computational chemistry is one of the most rapidly expanding and exciting areas of scientific endeavour in the medical, dental, and material sciences [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. The information available from computational methods may help interpret the molecular mechanism of interactions of 2-methoxy phenol antioxidants.…”

Section: Introductionmentioning

confidence: 99%

Excess Properties and Theoretical Evaluation on Ternary Systems of 1-Pentanol in n-Hexane Solution with some Organic Compounds at 313 K Using Ultrasonic Technique

Ibrahim

Jeyakumar

Vinayagam

2021

J. Appl. Sol. Chem. Model.

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The parameters like density(ρ), ultrasonic velocity (U), and viscosity (η) were experimentally measured. The excess parameters like adiabatic compressibility, acoustic impedance, free volume, free length, internal pressure and ultrasonic velocity in addition to K value were computed from the experimental data. The variation of these parameters at a constant temperature for the three ternary systems, namely OMP + 1-pentanol+n-hexane, N, N-DMF + 1-pentanol+n-hexane, and MMP + 1-pentanol+n-hexane, have been discussed in the light of molecular interaction. Further, certain theoretical studies are validated with respect to experimental velocities, and the Chi-square test for the goodness of fit is also applied to check the validity of such theoretical models.

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An ortho dimer of butylated hydroxyanisole inhibits nuclear factor kappa B activation and gene expression of inflammatory cytokines in macrophages stimulated by Porphyromonas gingivalis fimbriae

Cited by 6 publications

References 26 publications

Tobacco Upregulates P. gingivalis Fimbrial Proteins Which Induce TLR2 Hyposensitivity

Tobacco Upregulates P. gingivalis Fimbrial Proteins Which Induce TLR2 Hyposensitivity

Porphyromonas gingivalis Fimbria-Induced Expression of Inflammatory Cytokines and Cyclooxygenase-2 in Mouse Macrophages and Its Inhibition by the Bioactive Compounds Fibronectin and Melatonin

Excess Properties and Theoretical Evaluation on Ternary Systems of 1-Pentanol in n-Hexane Solution with some Organic Compounds at 313 K Using Ultrasonic Technique

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