Interleukin-3 enhances the migration of human mesenchymal stem cells by regulating expression of CXCR4

Barhanpurkar-Naik, Amruta; Mhaske, Suhas T.; Pote, Satish T.; Singh, Kanupriya; Wani, Mohan R.

doi:10.1186/s13287-017-0618-y

Cited by 41 publications

(39 citation statements)

References 60 publications

(71 reference statements)

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“…On the background of their previous report that, IL-3 prevents bone and cartilage damage, and increases the differentiation potential of MSCs into functional osteoblasts, Barhanpurkar-Naik et al [83] further investigate the role of IL-3 in the migration of MSCs. They report that MSCs conditioned with IL-3, overexpress CXCR4 which causes increased migration towards SDF-1α (i.e.…”

Section: Improving Migrationmentioning

confidence: 99%

Improved therapeutics of modified mesenchymal stem cells: an update

Pei²,

et al. 2020

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Background: Mesenchymal stromal cells (MSCs) have attracted intense interest due to their powerful intrinsic properties of self-regeneration, immunomodulation and multi-potency, as well as being readily available and easy to isolate and culture. Notwithstanding, MSC based therapy suffers reduced efficacy due to several challenges which include unfavorable microenvironmental factors in vitro and in vivo. Body: In the quest to circumvent these challenges, several modification techniques have been applied to the naïve MSC to improve its inherent therapeutic properties. These modification approaches can be broadly divided into two groups to include genetic modification and preconditioning modification (using drugs, growth factors and other molecules). This field has witnessed great progress and continues to gather interest and novelty. We review these innovative approaches in not only maintaining, but also enhancing the inherent biological activities and therapeutics of MSCs with respect to migration, homing to target site, adhesion, survival and reduced premature senescence. We discuss the application of the improved modified MSC in some selected human diseases. Possible ways of yet better enhancing the therapeutic outcome and overcoming challenges of MSC modification in the future are also elaborated. Conclusion: The importance of prosurvival and promigratory abilities of MSCs in their therapeutic applications can never be overemphasized. These abilities are maintained and even further enhanced via MSC modifications against the inhospitable microenvironment during culture and transplantation. This is a turning point in MSC-based therapy with promising preclinical studies and higher future prospect.

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Section: Improving Migrationmentioning

confidence: 99%

Improved therapeutics of modified mesenchymal stem cells: an update

Pei²,

et al. 2020

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“…Different molecules are involved in or necessary for the different steps in the homing process, and chemokines receptors (G‐protein coupled receptors) represent the main group . It has been extensively demonstrated that the CXCR4–stromal‐derived factor‐1 (CXCL12/SDF‐1) axis is critical for bone marrow homing, and a number of the other cytokines and growth factors such as CCL2/MCP1, TNFα, CXCL10/IP10 have been shown to increase migration in vitro …”

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confidence: 99%

Osteoarthritic Milieu Affects Adipose‐Derived Mesenchymal Stromal Cells

Manferdini

Paolella

Gabusi

et al. 2019

Journal Orthopaedic Research

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The objective of this study was to define the effects of osteoarthritic (OA) milieu on good manufactured practice-adipose-derived mesenchymal stromal cells (GMP-ASC) that are commonly utilized in cell therapies. Two different OA milieu: OA synovial fluid (SF) and OAconditioned medium (CM) from synoviocytes were used to treat GMP-ASC both in normoxia or hypoxia. GMP-ASC were tested for cell migration, proliferation, cytokine receptors expression (CXCR1, CXCR2, CXCR3, CXCR4, CXCR7, CCR1, CCR2, CCR3, CCR5, IL6R), and cytokines (CXCL8/IL8, CXCL10/IP10, CXCL12/SDF-1, CCL2/MCP1, CCL3/MIP1α, CCL4/MIP1β, CCL5/RANTES, IL6) release. Healthy SF was used as controls. We demonstrated that GMP-ASC show an increase in proliferation, migration, and modulation of CXCR1, CXCR3, CCR1, and CCR5 receptors in hypoxic condition. Moreover, GMP-ASC migration increased 15-fold when treated either with OA-SF or OA-CM compared with healthy SF both in normoxia and hypoxia. GMP-ASC treated in both OA milieu showed an increase in CXCR3, CCR3, and IL6R and a decrease in CCR1 and CCR2 receptors. In OA-SF, we detected higher amount of CXCL10/IP10 than in OA-CM, while CCL2/MCP1 and CCL4/MIP1β were higher in OA-CM compared with OA-SF. CXCL10/IP10 was the only chemokine of the OA milieu, which was downmodulated after treatment with GMP-ASC. In conclusion, we demonstrated specific effects of OA milieu on both GMP-ASC proliferation, migration, and cytokine receptor expression that were strictly dependent on the inflammatory and hypoxic environment. The use of characterized OA milieu is crucial to define the therapeutic effect of GMP-ASC and indicates that CXCL10/IP10-CXCR3 axis is partially involved in the GMP-ASC effect on synovial macrophages.

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“…It is well known that exposure of mesenchymal stem cells with IL-3 could increase cell migration and wound closure. On the other hand, Barhanpurkar-Naik showed that IL-3 stimulates cell migration in the mesenchymal stem and also fastens wound closure [33]. In this context, the level of the IL-3 was higher in the REO-NLCs-treated animals.…”

Section: Discussionmentioning

confidence: 89%

“…REO-NLCs increased SDF-1a in comparison to the control group. It is accepted that SDF-1a is significantly increased in the injured tissues and facilitates the migration and engraftment of circulation of CXCR4-positive cells [33]. With regards to increasing the level of IL-3 and SDF-1a in REO-NLCs-treated animals, it can be claimed that therapeutic gels helped to trigger the wound healing.…”

Section: Discussionmentioning

confidence: 99%

Accelerated infected wound healing by topical application of encapsulated Rosemary essential oil into nanostructured lipid carriers

Khezri

Farahpour

Rad

2019

Artificial Cells, Nanomedicine, and Biotechnology

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The pathogenic bacteria delay wound healing due to their interaction in the wound area. This study is aimed to evaluate the efficiency of topical rosemary essential oil (REO) loaded into the nanostructured lipid carriers (NLCs) on in vitro antibacterial activity and in vivo infected wound healing process in the animal model. REO-NLCs morphology, size and in vitro antibacterial activity were done. Two circular full-thickness wound (each 6 mm) were made on the back of each mouse and each wound was infected with a solution containing 10 7 CFU Staphylococcus aureus and Pseudomonas aeruginosa. Animals were divided into four groups including control, Mupirocin V R and two treated groups with a gel containing REO and REO-NLCs. For this purpose, tissue bacterial count, histological assessment, serum level of IL-3, IL-10, VEGF and SDF-1a were evaluated. REO-NLCs showed antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Pseudomonas aeruginosa. Moreover, REO-NLCs could reduce the rate of tissue bacterial colonization and wound size, while they increased the vascularization, fibroblast infiltration, re-epithelialization, collagen production, IL-3, IL-10, VEGF and SDF-1a serum levels. Our finding revealed the REO-NLCs have antibacterial properties and accelerated infected wound healing, and so that confirming their potential clinical uses for the treatment of infected wounds.

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Interleukin-3 enhances the migration of human mesenchymal stem cells by regulating expression of CXCR4

Cited by 41 publications

References 60 publications

Improved therapeutics of modified mesenchymal stem cells: an update

Improved therapeutics of modified mesenchymal stem cells: an update

Osteoarthritic Milieu Affects Adipose‐Derived Mesenchymal Stromal Cells

Accelerated infected wound healing by topical application of encapsulated Rosemary essential oil into nanostructured lipid carriers

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