Interleukin-1β and surface marker expression changes induced by tetrachloroplatinate in human monocyte-derived dendritic cells

Arkusz, Joanna; Gradecka-Meesters, Dobrosława; Stępnik, Maciej

doi:10.1080/08923970903095306

Cited by 2 publications

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“…Cells exhibited the typical markers of mature DCs as determined by flow cytometry detection. The antibodies used to characterize mature DC included anti‐CD40‐PE (BioSource, Zoersel, Belgium), anti‐HLA‐DR‐PE (Becton Dickinson, Erembodegem, Belgium), anti‐CD83‐PE (Dako, Glostrup, Denmark) and anti‐CD86‐FITC (Pharmingen, San Diego, CA, USA) 19–22 . Nonreactive isotype‐matched antibodies (Becton Dickinson) were obtained as controls.…”

Section: Methodsmentioning

confidence: 99%

Human dendritic cells transfected with amplified MUC1 mRNA stimulate cytotoxic T lymphocyte responses against pancreatic cancer in vitro

Jiang¹,

Li²,

Wang³

et al. 2011

J of Gastro and Hepatol

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Section: Methodsmentioning

confidence: 99%

Human dendritic cells transfected with amplified MUC1 mRNA stimulate cytotoxic T lymphocyte responses against pancreatic cancer in vitro

Jiang¹,

Li²,

Wang³

et al. 2011

J of Gastro and Hepatol

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“…In human MDDC, CD80, CD86, CD54, human leukocyte antigen-DR isotype (HLA-DR), and MHC II have all been reported to become upregulated in response to exposure to sensitizing metals (131,1043). Many metal nanomaterials, including AuNP, GdNP, SiNP, and a PtNP-containing agent have all been reported to induce elevated expression of these molecules in MDDC following in vitro exposure (538,953,1044,1045).…”

Section: Metal Nanomaterials Effects On DC and Sensitization In Vitromentioning

confidence: 99%

Metal Nanomaterials: Immune Effects and Implications of Physicochemical Properties on Sensitization, Elicitation, and Augmentation of Allergic Disease

Roach¹

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The recent surge in incorporation of engineered metallic and metal oxide nanomaterials into consumer products and their corresponding use in occupational settings has raised concerns over their potential to induce material size-specific toxicological effects. Although metal nanomaterials have been shown to induce greater degrees of lung injury and inflammation than their larger metal counterparts, their size-related effects on the immune system and allergic disease remain largely unknown. This knowledge gap is particularly concerning since metals are common inducers of allergic contact dermatitis, occupational asthma, and allergic adjuvancy.Overall, more scientific knowledge exists regarding the potential for metal-containing nanomaterials to exacerbate allergic disease than to their potential to induce allergic disease. Furthermore, effects of metal nanomaterial exposure on respiratory allergy have been more thoroughly-characterized than their potential influence on dermal allergy. Despite the existence of such knowledge, specific correlations between metal nanomaterial physico-chemical properties and their allergic effects have yet to be consistently demonstrated. As the number of emerging nanomaterials continues to increase, the delineation of these relationships has the potential to advance risk assessment efforts by helping to identify specific agents that may present an elevated risk for allergic effects.Two sets of studies were designed to begin addressing some of the knowledge gaps associated with the immunotoxic potential of metal nanomaterials in the context of allergic disease. The hypothesis of these studies was that nanomaterials comprised of metal constituents with known immunomodulatory potential augment, induce, and elicit allergic disease more readily than larger forms of the materials. Moreover, the nature of the immune responses caused by exposure to these metal nanomaterials, as well as the magnitude of the effects, correlates best with the parameter of dose surface area.The first set of studies incorporated NiO particles with different physico-chemical properties into an in vivo time course study and OVA asthma model. Results from the time course study demonstrated that the smaller NiO particles caused more pronounced pulmonary injury and inflammation, a discrepancy that could be mitigated by normalizing the administered particle dose to the surface area of the larger material. In the OVA model, augmentation of the allergic response was largely conserved with respect to NiO surface area, wherein larger doses caused polarization of pulmonary immune reposes towards a Th1/17-dominant state and smaller doses induced Th2-skewed responses. Despite this association, several immune markers, including total IgE production, BAL eosinophil number, and Penh response correlated better with other metrics, such as particle size.The second set of studies employed Au particles and nanoparticles to assess size-specific differences in allergenic potential. Neither particle was associated with potential for skin...

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Interleukin-1β and surface marker expression changes induced by tetrachloroplatinate in human monocyte-derived dendritic cells

Cited by 2 publications

References 0 publications

Human dendritic cells transfected with amplified MUC1 mRNA stimulate cytotoxic T lymphocyte responses against pancreatic cancer in vitro

Human dendritic cells transfected with amplified MUC1 mRNA stimulate cytotoxic T lymphocyte responses against pancreatic cancer in vitro

Metal Nanomaterials: Immune Effects and Implications of Physicochemical Properties on Sensitization, Elicitation, and Augmentation of Allergic Disease

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