Interleukin-1β Activity and Collagen Synthesis in Human Dental Pulp Fibroblasts

Abstract

Immunopathologic reactions play a significant role in inflammatory diseases of dental pulp. Interleukin-1beta (IL-1beta) is recognized as a key player in mediating cellular immune response. In this study, we measured the content of IL-1beta and its effect on collagen synthesis in cultures of fibroblasts derived from healthy and diseased dental pulps. We found that diseased pulp fibroblasts contain 2.5-fold greater amounts of IL-1beta and synthesized 80% greater amounts of collagen compared with healthy pulp fibroblasts. However, exogenous IL-1beta failed to stimulate collagen synthesis by diseased fibroblasts, whereas collagen synthesis by healthy pulp fibroblasts was stimulated by more than 2-fold. These observations imply that pulp disease induces abnormalities associated with fibroblast response toward IL-1beta.

Neuropeptides stimulate production of interleukin-1�, interleukin-6, and tumor necrosis factor-a in human dental pulp cells

“…In addition, Barkhordar et al (2002) found that diseased pulp fibroblasts contained 2.5-fold greater amounts of IL-1b when compared with healthy pulp fibroblasts. Furthermore, high levels of IL-6 have been connected with the pathogenesis of various inflammatory diseases, such as rheumatoid arthritis (Houssiau et al, 1988) and periodontitis (Reinhardt et al, 1993).…”

Pulp capping materials exert an effect on the secretion of IL-1β and IL-8 by migrating human neutrophils

“…Thus, the fact that MTA induces neutrophils to produce IL-1β could result in a more organized dental pulp response after the injury, leading to a more favorable pulp repair. 19 All tested materials induced the production of IL-8. This is an important finding, since it is known that all materials can induce different levels of acute inflammation in the pulp.…”

“…18 The production of IL-1β was increased only in the presence of the MTA. In fact, other studies have already reported in vitro 1,[17][18][19][20] and in vivo 12 that MTA is able to improve the production of IL-1β by macrophages, neutrophils and mast cells. This could have implications on the satisfactory effect of MTA after dental pulp exposure.…”

MicroRNA-146a and microRNA-155 show tissue-dependent expression in dental pulp, gingival and periodontal ligament fibroblasts in vitro

“…Moreover, once activated, fibroblasts may take on new patterns of extracellular matrix remodeling (42,43) or even assume new functions, as in the case of cytokine production (3,44). Smith et al (1) have highlighted differences among fibroblasts from distinct tissues as a possible determinant of local susceptibility to disease.…”