Interleukin 1α and 1β gene variations are associated with tuberculosis in silica exposed subjects

Salum, Kaio Cezar Rodrigues; Castro, Marcos César Santos de; Moreira, Valéria Barbosa; Nani, Ângela Santos Ferreira; Kohlrausch, Fabiana B.

doi:10.1002/ajim.23066

Cited by 11 publications

(5 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Reduced levels of GM-CSF were found in macrophages isolated from the peripheral blood of patients with active tuberculosis, and the killing capacity of the cells for Mtb was significantly reduced ( Mishra et al., 2022 ). IL-1α and IL-1β gene variants may somewhat increase TB susceptibility ( Salum et al., 2020 ). Producing the pro-inflammatory cytokines IL-1β and TNF-α helps inhibit Mtb infection in granulomas and prevent the spread of Mtb.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of human peripheral blood mononuclear cells stimulated by Mycobacterium tuberculosis antigen

Wei,

Guo,

Song

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

BackgroundMycobacterium tuberculosis antigen (Mtb-Ag) is a polypeptide component with a molecular weight of 10-14 kDa that is obtained from the supernatant of the H37Ra strain after heat treatment. It stimulates the activation and proliferation of γδT cells in the blood to produce an immune response against tuberculosis. Mtb-Ag is therefore crucial for classifying and detecting the central genes and key pathways involved in TB initiation and progression.MethodsIn this study, we performed high-throughput RNA sequencing of peripheral blood mononuclear cells (PBMC) from Mtb-Ag-stimulated and control samples to identify differentially expressed genes and used them for gene ontology (GO) and a Kyoto Encyclopedia of Genomes (KEGG) enrichment analysis. Meanwhile, we used PPI protein interaction network and Cytoscape analysis to identify key genes and qRT-PCR to verify differential gene expression. Single-gene enrichment analysis (GSEA) was used further to elucidate the potential biological functions of key genes. Analysis of immune cell infiltration and correlation of key genes with immune cells after Mtb-Ag-stimulated using R language.ResultsWe identified 597 differentially expressed genes in Mtb-Ag stimulated PBMCs. KEGG and GSEA enrichment analyzed the cellular pathways related to immune function, and DEGs were found to be primarily involved in the TNF signaling pathway, the IL-17 signaling pathway, the JAK-STAT signaling pathway, cytokine-cytokine receptor interactions, and the NF-κB signaling pathway. Wayne analysis using GSEA, KEGG, and the protein-protein interaction (PPI) network showed that 34 genes, including PTGS2, IL-1β, IL-6, TNF and IFN-γ et al., were co-expressed in the five pathways and all were up-regulated by Mtb-Ag stimulation. Twenty-four DEGs were identified using qRT-PCR, including fourteen up-regulated genes (SERPINB7, IL20, IFNG, CSF2, PTGS2, TNF-α, IL36G, IL6, IL10, IL1A, CXCL1, CXCL8, IL4, and CXCL3) and ten down-regulated genes (RTN1, CSF1R CD14, C5AR1, CXCL16, PLXNB2, OLIG1, EEPD1, ENG, and CCR1). These findings were consistent with the RNA-Seq results.ConclusionThe transcriptomic features associated with Mtb-Ag provide the scientific basis for exploring the intracellular immune mechanisms against Mtb. However, more studies on these DEGs in pathways associated with Mtb-Ag stimulation are needed to elucidate the underlying pathologic mechanisms of Mtb-Ag during Mtb infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of human peripheral blood mononuclear cells stimulated by Mycobacterium tuberculosis antigen

Wei,

Guo,

Song

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…A doença pode evoluir, nas fases avançadas, para quadro de insuficiência respiratória, perda de peso e fraqueza generalizada, tornando o paciente mais suscetível a infecções do trato respiratório inferior. Sempre é importante lembrar que a tuberculose apresenta elevada prevalência nesses pacientes (Fernandez Ana Carolina Machado Guimarães Gonçalves Marques, David Versalli Souza, Henrique Melo Xavier, Isabela Pinto de Medeiros, Angela Santos Ferreira Nani, Marcos César Santos de Castro ____________________________________________________________________________ Alvarez et al, 2015;Hoy and Chambers, 2020;Krefft et al, 2020;Salum et al, 2020b).…”

Section: Introductionunclassified

Existe Diferença Na Função Pulmonar Entre Pacientes Com Silicose Simples E Complicada?

Marques,

Souza,

Xavier

et al. 2024

Rev. Foco

Self Cite

View full text Add to dashboard Cite

Introdução: A silicose é uma doença pulmonar fibrosante e irreversível, causada pela inalação de cristais de sílica. A exposição à sílica é um fator independente para o enfisema pulmonar. Diversos autores descrevem que o maior comprometimento do parênquima pulmonar pela silicose ocasionaria um maior impacto funcional pulmonar. Entretanto, há estudos com resultados controversos sobre esta associação. Objetivos: Comparar os parâmetros funcionais (CVF%, VEF1/CVF e VEF1%) entre pacientes com silicose simples e complicada em acompanhamento regular no ambulatório de doenças ocupacionais do HUAP/UFF. Métodos: Trata-se de um estudo transversal onde foram avaliados os parâmetros funcionais em 78 pacientes com silicose. Os pacientes foram classificados em silicose simples ou complicada de acordo com a classificação das radiografias das pneumoconioses da Organização Internacional do Trabalho. Foram comparadas as médias dos parâmetros funcionais: CVF(%), VEF1/CVF e VEF1(%) entre pacientes com silicose simples e complicada. Foi utilizado para análise estatística o software SPSS 22.0, sendo considerado resultado com significância estatística p<0,05. O teste de Kolmogorov-Smirnov evidenciou amostra com distribuição normal (p>0,05). O tempo de exposição à sílica e as horas semanais trabalhadas também foram comparados entre os dois grupos. Resultados: Todos os 78 pacientes eram do sexo masculino, com médias de idade de 59,52±7,97 anos e IMC de 23,52±3,46kg/m². Os pacientes foram classificados em silicose simples e complicada, sendo 26 (33%) com silicose simples, 52 (67%) silicose complicada. A atividade profissional mais prevalente foi jateamento de areia, com 48 (62%) pacientes. O tempo de exposição total foi de 21,48±8,35 anos (p=0,313) e as horas semanais trabalhadas de 47,82±9,68 horas (p=0,743), não havendo diferença significativa entre os dois grupos. Do total da amostra, 41 (52%) tinham história prévia de tabagismo e carga tabágica de 38,09±41,04 maços/ano, sem diferença significativa entre os grupos (p=0,265). Os parâmetros funcionais encontrados na silicose simples foram de CVF(%) 88,94±22,35, VEF1/CVF(%) 70,29±9,97, VEF1(%) 78,28±24,51. Os parâmetros funcionais encontrados na silicose complicada foram de CVF (%) 73,47±16,47, VEF1/CVF(%): 63,57±15,42, VEF1(%): 57,43±20,92. Foi observada diferença com significância estatística entre silicose simples e complicada para os parâmetros CVF(%) (p=0,001), VEF1/CVF (p=0,047) e VEF1(%) (p=0,0001). Conclusão: Nesta amostra pacientes com silicose complicada apresentaram menores parâmetros funcionais quando comparados aos pacientes com silicose simples. Neste trabalho, a extensão da doença parenquimatosa interferiu na perda da função pulmonar.

show abstract

“…This occurs because tuberculosis and silicosis both present clinically with cough and dyspnea and has a radiological characteristic of upper lobe predominant centrilobular nodules. 5,10,15,16 In this article, we describe the case of a patient with silicotuberculosis, where the radiological aspect was fundamental for the diagnostic suspicion.…”

Section: Introductionmentioning

confidence: 99%

The importance of tomographic findings for the diagnosis of silicotuberculosis: case report

Castro¹,

Moreira²,

Nani³

et al. 2023

MOJCR

Self Cite

View full text Add to dashboard Cite

Silicosis is a pneumoconiosis characterized by fibrosis of the lung parenchyma caused by the inhalation of silica dust. Tuberculosis is an important and frequent infectious complication of silicosis, called silicotuberculosis. The risk of pulmonary tuberculosis in patients with silicosis is up to 40 times higher when compared to the general population. As the radiological findings are similar in both diseases, the diagnosis of silicotuberculosis is not always simple. We describe the case of a patient with silicosis associated with clinical and radiological findings compatible with tuberculosis.

show abstract

Interleukin 1α and 1β gene variations are associated with tuberculosis in silica exposed subjects

Cited by 11 publications

References 112 publications

Transcriptional analysis of human peripheral blood mononuclear cells stimulated by Mycobacterium tuberculosis antigen

Transcriptional analysis of human peripheral blood mononuclear cells stimulated by Mycobacterium tuberculosis antigen

Existe Diferença Na Função Pulmonar Entre Pacientes Com Silicose Simples E Complicada?

The importance of tomographic findings for the diagnosis of silicotuberculosis: case report

Contact Info

Product

Resources

About