Interleukin‐10 production by a B‐cell line derived from human post‐transplant lymphoproliferative disease

Abstract

Interleukin-10 (IL-10) is a cytokine known to regulate growth and differentiation in activated human B cells. We studied IL-10 production in a B-cell line derived from Epstein-Barr virus associated post-transplant lymphoproliferative disease (PTLD). Reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated IL-10 mRNA within the cells. Transcripts of the virally encoded homologue BCRF-1 were not detected. ELISA assays demonstrated translation of IL-10 message into the corresponding cytokine, and its subsequent secretion into the culture medium. The rate of 3H-thymidine incorporation by the PTLD cells was not affected by immunologic neutralization of the secreted cytokine, or, by addition of exogenous recombinant IL-10 to the culture medium. Thus, IL-10 does not have an autocrine growth regulatory role in this PTLD line.

In vitro culture of B-lymphocytes derived from epstein-barr-virus-associated posttransplant lymphoproliferative disease: Cytokine production and effect of interferon-alpha

“…The data presented here show that posttransplant lymphoproliferative diseasederived cells resemble LCLs and BL cell lines in that they are refractory to exogenously added interleukin-10. The lack of effect of anti-interleukin-10 on cell proliferation confirms our preliminary observation that interleukin-10 may not have an autocrine growth regulatory role in posttransplant lymphoproliferative disease (Randhawa et al, 1995). The immunosuppressive properties of interleukin-10, evidenced by its ability to suppress IL-1, IL-6, and TNF-a production by monocytes (Malefyt et al, 1991a;Malefyt et al, 1991b), could, however, interfere with host defense mechanisms directed against Epstein-Barr-virus-infected posttransplant lymphoproliferative disease cells in vivo.…”

Mycophenolate mofetil-based immunosuppression and cytokine genotypes: effects on monokine secretion and antigen presentation in long-term renal transplant recipients

“…Thus, MMF treatment with or without the addition of steroids may be expected to have beneficial effects not only on acute but also on chronic rejection as a result of suppression of IL-10 secretion. Furthermore, MMF might reduce the incidence of PTLD because IL-10 seems to play a role in Epstein Barr virus-associated PTLD (41)(42)(43).…”

T Cells Overexpressing Interferon‐γ and Interleukin‐10 Are Found in Both the Thymus and Secondary Lymphoid Tissues of Feline Immunodeficiency Virus–Infected Cats

“…Studies have shown that the earliest T cell precursor in the thymus has the capacity to generate into B cells [40,41], and IL-10 also regulates human B cell growth and differentiation [42]. It has been speculated that chronic overexpression of IL-10 may play a central role in the pathogenesis at University of North Dakota on June 4, 2015 http://jid.oxfordjournals.org/ Downloaded from of B cell lymphomas [43], which are characteristic of late-stage infection with both HIV and FIV.…”