Interlaboratory studies on in vitro test methods for estimating in vivo resorption of calcium phosphate ceramics

Ito, Atsuo; Sogo, Yu; Yamazaki, Atsushi; Aizawa, Mamoru; Osaka, Akiyoshi; Hayakawa, Satoshi; Kikuchi, Masanori; Yamashita, Kimihiro; Tanaka, Yumi; Tadokoro, Mika; Sena, Lídia Ágata de; Buchanan, Fraser; Ohgushi, Hajime; Bohner, Marc

doi:10.1016/j.actbio.2015.07.040

Cited by 27 publications

(13 citation statements)

References 41 publications

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“…For each samples, sections (35 μm thick) were cut and stained with hematoxylin and eosin (H&E). Newly formed bone area was measured by light microscopy and then quantified using a computer‐assisted Image‐Pro Plus System (Media Cybernetics, Silver Springs, MD) …”

Section: Methodssupporting

confidence: 72%

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Zirconium modified calcium‐silicate‐based nanoceramics: An in vivo evaluation in a rabbit tibial defect model

Doostmohammadi

Esfahani

Ardeshirylajimi

et al. 2018

Int J Applied Ceramic Tech

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Over the past two decades calcium silicate (Ca-Si) based ceramics have been introduced as bioactive materials for bone tissue engineering applications and repairing bone defects. Recently, it is reported that zirconium modified calciumsilicate-based (Ca 3 ZrSi 2 O 9 , Baghdadite) ceramics stimulate cell proliferation and osteogenic differentiation of osteoblasts. The aim of this work was preparation, characterization, and evaluation of biological properties of Baghdadite in the nanoparticles form. These nanoparticles were synthesized by sol-gel method and were annealed at 1150°C. Fabricated nanoparticles were investigated using XRD, FE-SEM, EDX, and MTT in vitro, then implanted to the rabbit tibia as a bone animal model. The characterization results showed that nanoparticles with expected composition were successfully synthesized using sol-gel method. According to MTT result, these nanoparticles were nontoxic. In addition, the proliferation of bone marrow derived mesenchymal stem cells was increased after 96 hours of culturing in the presence of nanoparticles compared to the control. Histopathological results also demonstrated that the defected bone was completely regenerated when implanted with nanoparticles after 6 weeks. Taking together, according to the results, Ca 3 ZrSi 2 O 9 nanoparticles as a bioceramic with excellent biological properties can be applied for improvement of bone lesions healing process in the orthopedic and dental application. K E Y W O R D S bioactivity, bioceramics, calcium silicate, nanoparticle How to cite this article: Doostmohammadi A, Karimzadeh Esfahani Z, Ardeshirylajimi A, Rahmati Dehkordi Z. Zirconium modified calcium-silicatebased nanoceramics: An in vivo evaluation in a rabbit tibial defect model. Int J Appl Ceram Technol. 2019;16:431-437. https://doi.

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Section: Methodssupporting

confidence: 72%

“…In the first 24 hours after surgery, with intervals of 8 hours, one milligram of Ceftiofur antibiotic was prescribed intramuscularly. After the surgery and each day, the surgical site in body's rabbit was controlled to insure whether there is any infection or not …”

Section: Methodsmentioning

confidence: 99%

Zirconium modified calcium‐silicate‐based nanoceramics: An in vivo evaluation in a rabbit tibial defect model

Doostmohammadi

Esfahani

Ardeshirylajimi

et al. 2018

Int J Applied Ceramic Tech

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“…The relative solubilities of the different compositions were assessed by incubating as-prepared powder samples (sieved to 53-315 mm size) in an acidic (pH 5.5) buffer solution for 30 minutes and measuring the Ca ion concentration, based on a method described by Mano et al, 37 For each composition studied, 30 mg of sample was incubated in 20 ml of 0.08 M sodium acetate-acetic acid buffer solution, prepared as described elsewhere, 38 for 30 minutes at 20 1C, under 150 rpm mixing. A 100 ml aliquot was collected for the measurement of Ca ion concentration in the buffer solution.…”

Section: Dissolution Evaluationmentioning

confidence: 99%

Potassium–carbonate co-substituted hydroxyapatite compositions: maximising the level of carbonate uptake for potential CO₂ utilisation options

2022

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“…The purpose of the present study was, therefore, to investigate the degradation/ dissolution behavior of PMCs in physiological fluids, ex vivo. The cell-mediated resorption rate, in vitro [64] and in vivo [65], is directly related to phase solubility. Since phosphoserine stabilizes metastable phases (αTCP) in liquid, by preventing or delaying dissolution/reprecipitation of αTCP, it is necessary to determine whether the in vivo dissolution and remodeling rate is also delayed.…”

Section: Introductionmentioning

confidence: 99%

Phosphoserine Functionalized Cements Preserve Metastable Phases, and Reprecipitate Octacalcium Phosphate, Hydroxyapatite, Dicalcium Phosphate, and Amorphous Calcium Phosphate, during Degradation, In Vitro

Byström

Pujari-Palmer

2019

JFB

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Phosphoserine modified cements (PMC) exhibit unique properties, including strong adhesion to tissues and biomaterials. While TTCP-PMCs remodel into bone in vivo, little is known regarding the bioactivity and physiochemical changes that occur during resorption. In the present study, changes in the mechanical strength and composition were evaluated for 28 days, for three formulations of αTCP based PMCs. PMCs were significantly stronger than unmodified cement (38–49 MPa vs. 10 MPa). Inclusion of wollastonite in PMCs appeared to accelerate the conversion to hydroxyapatite, coincident with slight decrease in strength. In non-wollastonite PMCs the initial compressive strength did not change after 28 days in PBS (p > 0.99). Dissolution/degradation of PMC was evaluated in acidic (pH 2.7, pH 4.0), and supersaturated fluids (simulated body fluid (SBF)). PMCs exhibited comparable mass loss (<15%) after 14 days, regardless of pH and ionic concentration. Electron microscopy, infrared spectroscopy, and X-ray analysis revealed that significant amounts of brushite, octacalcium phosphate, and hydroxyapatite reprecipitated, following dissolution in acidic conditions (pH 2.7), while amorphous calcium phosphate formed in SBF. In conclusion, PMC surfaces remodel into metastable precursors to hydroxyapatite, in both acidic and neutral environments. By tuning the composition of PMCs, durable strength in fluids, and rapid transformation can be obtained.

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Interlaboratory studies on in vitro test methods for estimating in vivo resorption of calcium phosphate ceramics

Cited by 27 publications

References 41 publications

Zirconium modified calcium‐silicate‐based nanoceramics: An in vivo evaluation in a rabbit tibial defect model

Zirconium modified calcium‐silicate‐based nanoceramics: An in vivo evaluation in a rabbit tibial defect model

Potassium–carbonate co-substituted hydroxyapatite compositions: maximising the level of carbonate uptake for potential CO₂ utilisation options

Phosphoserine Functionalized Cements Preserve Metastable Phases, and Reprecipitate Octacalcium Phosphate, Hydroxyapatite, Dicalcium Phosphate, and Amorphous Calcium Phosphate, during Degradation, In Vitro

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Interlaboratory studies on in vitro test methods for estimating in vivo resorption of calcium phosphate ceramics

Cited by 27 publications

References 41 publications

Zirconium modified calcium‐silicate‐based nanoceramics: An in vivo evaluation in a rabbit tibial defect model

Zirconium modified calcium‐silicate‐based nanoceramics: An in vivo evaluation in a rabbit tibial defect model

Potassium–carbonate co-substituted hydroxyapatite compositions: maximising the level of carbonate uptake for potential CO2 utilisation options

Phosphoserine Functionalized Cements Preserve Metastable Phases, and Reprecipitate Octacalcium Phosphate, Hydroxyapatite, Dicalcium Phosphate, and Amorphous Calcium Phosphate, during Degradation, In Vitro

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Potassium–carbonate co-substituted hydroxyapatite compositions: maximising the level of carbonate uptake for potential CO₂ utilisation options