Intergenic region typing (IRT): A rapid molecular approach to the characterization and evolution of Leishmania

Ecology of the Leishmania Species in the Guianan Ecoregion Complex

“…However, isolates from different polymorphic species show plesiomorphic similarities in their enzymatic or genetic profiles, suggesting that they may have originated from one primary sylvatic enzootic form. 80,81 Globally, although L. (V.) braziliensis [82][83][84][85][86][87][88][89][90] and in a lesser extent L. (V.) amazonensis 85,91 were shown to be highly polymorphic, L. (V.) naiffi, 45,82,83 L. (V.) guyanensis, 46,83,88 and L. (L.) deanei 92 were shown to have less diversity. Leishmania (V.) lainsoni was shown to be more invariant.…”

“…Leishmania (V.) lainsoni was shown to be more invariant. 83 Several studies have discussed the polymorphism observed in natural populations of different Leishmania species. [93][94][95][96][97] Until now, there has been little information available about the genetic variability of the parasites and its correlation with the ecoepidemiologic features of leishmaniasis 93,98 (Rotureau B, unpublished data).…”

Polymerase chain reaction with two molecular targets in mucosal leishmaniasis' diagnosis: a validation study

“…PCR -Each sample was analyzed using three different pairs of PCR primers: for ITS (internal transcript spacers) rDNA (Cupolillo et al 1995), a 1000 bp sequence was amplified with IR1 (5' -GCT GTA GGT GAA CCT GCA GCA GCT GGA TCA TT -3') and IR2 (5' -GCG GGT AGT CCT GCC AAA CAC TCA GGT CTG -3'); for nested ITS PCR, primers ITS 1F (5' -GCA GCT GGA TCA TTT TCC -3') and ITS 2R (5' -AAC ACT CAG GTC TGT AAA C -3') were used. In order to amplify a 120 bp sequence from the conserved region of kDNA (Rodgers et al 1990), a modified 13A (5' -TAG GGG CGT TCT GCG AA -3') and 13B (5' -ATT TTA CAC CAA CCC CCA GTT -3') primers were used.…”

Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA

“…Oligonulceotides were used to amplify the region corresponding to the 5.8S rDNA plus the two flanking ITS as described elsewhere (Cupolillo et al 1995, Fernandes et al 1999. The PCR products were further submitted to RFLP analysis with six endonucleases (Rsa I, Hae III, Eco RI, Taq I, Bst UI and Sau 3AI).…”